NF-κB信号通路在人羊膜间充质干细胞治疗宫腔粘连中的作用

勾亚婷; 张文文; 李长江; 于结; 毛艳华; 吴本媛; 张应凤; 陈芯培; 黄佳月; 孙聪聪; 王佳

doi:10.16016/j.1000-5404.202003065

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NF-κB信号通路在人羊膜间充质干细胞治疗宫腔粘连中的作用

基础医学 | 更新时间：2026-01-09

- NF-κB信号通路在人羊膜间充质干细胞治疗宫腔粘连中的作用
- Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells
- 第三军医大学学报 2020年42卷第11期页码：1101-1108
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市渝中区基础研究与前沿探索项目(20190122);重庆市研究生科研创新项目(CYS19195)
- DOI：10.16016/j.1000-5404.202003065
  中图分类号：
- 收稿：2020-03-05，
  
  纸质出版：2020-06-15
- 稿件说明：
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勾亚婷, 张文文, 李长江, 等. NF-κB信号通路在人羊膜间充质干细胞治疗宫腔粘连中的作用[J]. 第三军医大学学报, 2020,42(11):1101-1108.

Yating GOU, Wenwen ZHANG, Changjiang LI, et al. Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells[J]. Journal of Third Military Medical University, 2020, 42(11): 1101-1108.
勾亚婷, 张文文, 李长江, 等. NF-κB信号通路在人羊膜间充质干细胞治疗宫腔粘连中的作用[J]. 第三军医大学学报, 2020,42(11):1101-1108. DOI： 10.16016/j.1000-5404.202003065.

Yating GOU, Wenwen ZHANG, Changjiang LI, et al. Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells[J]. Journal of Third Military Medical University, 2020, 42(11): 1101-1108. DOI： 10.16016/j.1000-5404.202003065.

摘要

Abstract

Objective

To investigate the role of IκB-α/NF-κB signaling pathway in the treatment of intrauterine adhesions (IUA) by human amniotic mesenchymal stem cells (hAMSCs).

Methods

In vivo

experiment: 18 SD female rats were randomly divided into Sham group

IUA model group and hAMSCs group. The number of endometrial glands and the percentage of fibrotic area were calculated by HE staining and Masson staining

respectively. Immunohistochemistry was used to detect the expression levels of IκB-α (NF-κB inhibitor)

nuclear transfer factor P65

E-cadherin and vimentin in endometrial tissue.

In vitro

experiments: hAMSCs were induced by exogenous growth factors (10 ng/mL TGF-β1+EGF+PDGF-BB) and 10 nmol/L BAY11-7082 combined with above growth factors for 5 d

respectively

and the cells without treatment served as blank control. Fluorescence quantitative PCR was used to detect the expression levels of IκB-α

and cytokeratins CK-7 and CK-19.

Results

In vivo

experiment: The expression levels of IκB-α

P65 and vimentin was significant higher

while that of E-

cadherin was obviously lower in the model group than the Sham group (all

0.05); the expression levels of IκB-α

P65 and vimentin in the hAMSCs group were notably lower

while that of E-cadherin was higher than the model group (all

0.05).

In vitro

experiment: Compared with the control group

the expression of IκB-α was decreased but that of CK-7 was increased in the induction group; The expression levels of CK-7 and CK-19 were increased significantly in the inhibitor group than the induction group (both

0.05).

Conclusion

hAMSCs transplantation have therapeutic effect on uterine adhesions

which may be due to its promoting the transformation of hAMSCs into endometrial epithelial cells by inhibition of IκB-α/NF-κB pathway activation.

关键词

Keywords

references

ASHERMAN J G Amenorrhoea Traumatica (Atretica) J Obstet Gynaecol Br Emp 1948 55 1 23 30 10.1111/j.1471-0528.1948.tb07045.x ASHERMAN J G. Amenorrhoea Traumatica (Atretica)[J]. J Obstet Gynaecol Br Emp, 1948, 55(1):23-30.

阳媛 . 羊膜间充质干细胞移植联合雌激素治疗宫腔粘连的动物实验研究 [D ] . 重庆 : 重庆医科大学 , 2018 . http://cdmd.cnki.com.cn/Article/CDMD-10631-1018865411.htm 阳媛.羊膜间充质干细胞移植联合雌激素治疗宫腔粘连的动物实验研究[D].重庆: 重庆医科大学, 2018. http://cdmd.cnki.com.cn/Article/CDMD-10631-1018865411.htm

YANG Y . Human amniotic mesenchymal stem cells transplantation combined with estrogen in the treatment of intrauterine adhesions: a rat experimental study [D ] . Chongqing : Chongqing Medical University , 2018 . YANG Y. Human amniotic mesenchymal stem cells transplantation combined with estrogen in the treatment of intrauterine adhesions: a rat experimental study[D]. Chongqing: Chongqing Medical University, 2018.

毛艳华 . 羊膜间充质干细胞联合雌激素治疗宫腔粘连的体外实验研究 [D ] . 重庆 : 重庆医科大学 , 2018 . http://cdmd.cnki.com.cn/Article/CDMD-10631-1018865410.htm 毛艳华.羊膜间充质干细胞联合雌激素治疗宫腔粘连的体外实验研究[D].重庆: 重庆医科大学, 2018. http://cdmd.cnki.com.cn/Article/CDMD-10631-1018865410.htm

MAO Y H . In vitro experimental study of treatment effect of human amniotic mesenchymal stem cells combined with estrogen on intrauterine adhesions [D ] . Chongqing : Chongqing Medical University , 2018 . MAO Y H. In vitro experimental study of treatment effect of human amniotic mesenchymal stem cells combined with estrogen on intrauterine adhesions[D]. Chongqing: Chongqing Medical University, 2018.

丛姗宋瑾张惠娟人羊膜间充质干细胞(hAMSCs)的分离、体外培养及诱导分化农业生物技术学报 2015 23 1 20 31 10.3969/j.issn.1674-7968.2015.01.003.CONGS, 丛姗, 宋瑾, 张惠娟, 等.人羊膜间充质干细胞(hAMSCs)的分离、体外培养及诱导分化[J].农业生物技术学报, 2015, 23(1): 20-31. DOI:10.3969/j.issn.1674-7968.2015. 01.003.CONGS,

CONG S SONG J ZHANG H J Isolation and culture of human amniotic mesenchymal stem cells(hAMSCs) and its differentiation potential in vitro J Agric Biotechnol 2015 23 1 20 31 10.3969/j.issn.1674-7968.2015.01.003 CONG S, SONG J, ZHANG H J, et al . Isolation and culture of human amniotic mesenchymal stem cells(hAMSCs) and its differentiation potential in vitro [J ] . J Agric Biotechnol, 2015, 23(1): 20-31. DOI: 10.3969/j.issn.1674-7968.2015. 01.003.

刘晓丽王晶陈春林 Smad2、Erk2及NF-kB在宫腔粘连组织中表达的临床研究妇产与遗传(电子版) 2017 7 4 16 20 10.3868/j.issn.2095-1558.LIUXL, 刘晓丽, 王晶, 陈春林. Smad2、Erk2及NF-kB在宫腔粘连组织中表达的临床研究[J].妇产与遗传(电子版), 2017, 7(4): 16-20. DOI: 10.3868/j.issn.2095-1558.LIUX L,

LIU X L WANG J CHEN C L The expression of Smad2, Erk2, and NF-κB in endometrium of patient with intrauterine adhesion Obstet-Gynecol Genet Electron Ed 2017 7 4 16 20 10.3868/j.issn.2095-1558 LIU X L, WANG J, CHEN C L. The expression of Smad2, Erk2, and NF-κB in endometrium of patient with intrauterine adhesion[J]. Obstet-Gynecol Genet Electron Ed, 2017, 7(4): 16-20. DOI: 10.3868/j.issn.2095-1558.

马娜娜王祥珍核转录因子κb在宫腔粘连大鼠模型中的表达中国计划生育和妇产科 2014 6 2 61 64, 68 10.3969/j.issn.1674-4020.MANN, 马娜娜, 王祥珍.核转录因子κb在宫腔粘连大鼠模型中的表达[J].中国计划生育和妇产科, 2014, 6(2): 61-64, 68. DOI: 10.3969/j.issn.1674-4020.MAN N,

MA N N WANG X Z The expression of nuclear transcription factor κb in rat model of intrauterine adhesions Chin J Fam Plan Gynecotokol 2014 6 2 61 64, 68 10.3969/j.issn.1674-4020 MA N N, WANG X Z. The expression of nuclear transcription factor κb in rat model of intrauterine adhesions[J]. Chin J Fam Plan Gynecotokol, 2014, 6(2): 61-64, 68. DOI: 10.3969/j.issn.1674-4020.

韩华薛改李洁宫腔粘连模型大鼠子宫内膜胞饮突发育和整合素β3表达现代妇产科进展 2017 26 5 345 348 10.13283/j.cnki.xdfckjz.2017.05.007 韩华, 薛改, 李洁, 等.宫腔粘连模型大鼠子宫内膜胞饮突发育和整合素β3表达[J].现代妇产科进展, 2017, 26(5): 345-348. DOI:10.13283/j.cnki.xdfckjz.2017.05.007.

HAN H XUE G LI J The development of pinopodes and expression of integrin beta 3 in the endometrium of rat model of intrauterine adhesion Prog Obstet Gynecol 2017 26 5 345 348 10.13283/j.cnki.xdfckjz.2017.05.007 HAN H, XUE G, LI J, et al . The development of pinopodes and expression of integrin beta 3 in the endometrium of rat model of intrauterine adhesion[J ] . Prog Obstet Gynecol, 2017, 26(5): 345-348. DOI:10.13283/j.cnki.xdfckjz. 2017.05.007.

SUN L ZHANG S W CHANG Q Y Establishment and comparison of different intrauterine adhesion modelling procedures in rats Reprod Fertil Dev 2019 10.1071/RD18397 SUN L, ZHANG S W, CHANG Q Y, et al . Establishment and comparison of different intrauterine adhesion modelling procedures in rats[J ] . Reprod Fertil Dev, 2019. DOI: 10.1071/RD18397.

LIU X DUAN H ZHANG H H Integrated data set of micrornas and mRNAs involved in severe intrauterine adhesion Reprod Sci 2016 23 10 1340 1347 10.1177/1933719116638177 LIU X, DUAN H, ZHANG H H, et al . Integrated data set of micrornas and mRNAs involved in severe intrauterine adhesion [J ] . Reprod Sci, 2016, 23(10):1340-1347.DOI: 10.1177/1933719116638177.

SANTAMARIA X ISAACSON K SIMÓN C Asherman's syndrome: it may not be all our fault Hum Reprod 2018 33 8 1374 1380 10.1093/humrep/dey232 SANTAMARIA X, ISAACSON K, SIMÓN C. Asherman's syndrome: it may not be all our fault[J]. Hum Reprod, 2018, 33(8): 1374-1380. DOI:10.1093/humrep/dey232.

XUE X CHEN Q ZHAO G The overexpression of TGF-β and CCN2 in intrauterine adhesions involves the NF-κB signaling pathway PLoS ONE 2015 10 12 e0146159 10.1371/journal.pone.0146159 XUE X, CHEN Q, ZHAO G, et al . The overexpression of TGF-β and CCN2 in intrauterine adhesions involves the NF-κB signaling pathway[J ] . PLoS ONE, 2015, 10(12): e0146159. DOI:10.1371/journal.pone.0146159.

LEGLER D F MICHEAU O DOUCEY M A Recruitment of TNF receptor 1 to lipid rafts is essential for TNFα-mediated NF-κB activation Immunity 2003 18 5 655 664 10.1016/s1074-7613(03)00092-x LEGLER D F, MICHEAU O, DOUCEY M A, et al . Recruitment of TNF receptor 1 to lipid rafts is essential for TNFα-mediated NF-κB activation[J ] . Immunity, 2003, 18(5): 655-664. DOI:10.1016/s1074-7613(03)00092-x.

ASGAROVA A ASGAROV K GODET Y PD-L1 expression is regulated by both DNA methylation and NF-kB during EMT signaling in non-small cell lung carcinoma Oncoimmunology 2018 7 5 e1423170 10.1080/2162402X.2017.1423170 ASGAROVA A, ASGAROV K, GODET Y, et al . PD-L1 expression is regulated by both DNA methylation and NF-kB during EMT signaling in non-small cell lung carcinoma[J ] . Oncoimmunology, 2018, 7(5): e1423170. DOI:10.1080/2162402X.2017.1423170.

LI Y WANG L H ZHANG H T Disulfiram combined with copper inhibits metastasis and epithelial-mesenchymal transition in hepatocellular carcinoma through the NF-κB and TGF-β pathways J Cell Mol Med 2018 22 1 439 451 10.1111/jcmm.13334 LI Y, WANG L H, ZHANG H T, et al . Disulfiram combined with copper inhibits metastasis and epithelial-mesenchymal transition in hepatocellular carcinoma through the NF-κB and TGF-β pathways[J ] . J Cell Mol Med, 2018, 22(1): 439-451. DOI:10.1111/jcmm.13334.

KUPHAL S POSER I JOBIN C Loss of E-cadherin leads to upregulation of NF kappaB activity in malignant melanoma Oncogene 2004 23 52 8509 8519 10.1038/sj.onc.1207831 KUPHAL S, POSER I , JOBIN C, et al . Loss of E-cadherin leads to upregulation of NF kappaB activity in malignant melanoma[J ] . Oncogene, 2004, 23(52): 8509-8519. DOI:10.1038/sj.onc.1207831.

张雯碧程明军徐丛剑小鼠骨髓间充质干细胞向子宫内膜上皮细胞方向分化的体外实验现代妇产科进展 2010 19 4 257 260 10.13283/j.cnki.xdfckjz.2010.04.009 张雯碧, 程明军, 徐丛剑.小鼠骨髓间充质干细胞向子宫内膜上皮细胞方向分化的体外实验[J].现代妇产科进展, 2010, 19(4): 257-260. DOI:10.13283/j.cnki.xdfckjz. 2010.04.009.

ZHANG W B CHENG M J XU C J Differentiation of mice's marrow mesenchymal stem cells into endometrial epithelial cells in vitro Prog Obstet Gynecol 2010 19 4 257 260 10.13283/j.cnki.xdfckjz.2010.04.009 ZHANG W B, CHENG M J, XU C J. Differentiation of mice's marrow mesenchymal stem cells into endometrial epithelial cells in vitro [J ] . Prog Obstet Gynecol, 2010, 19(4): 257-260. DOI:10.13283/j.cnki.xdfckjz.2010.04.009.

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