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脂蛋白SA2275抗体治疗金黄色葡萄球菌脑脓肿的实验初探
Therapeutic effect of polyclonal lipoprotein SA2275 antibodies on
Staphylococcus aureus -induced brain abscess in mice- 第三军医大学学报 2020年42卷第16期 页码：1655-1662
- 作者机构：
1.650032 昆明，昆明医科大学第一附属医院神经内科
2.400038 重庆，陆军军医大学(第三军医大学)基础医学院微生物学教研室，重庆市微生物工程实验室
3.400715 重庆，西南大学生命科学学院, 现代生物医药研究所, 三峡库区生态环境与生物资源省部共建国家重点实验室培育基地
4.400037 重庆，陆军军医大学(第三军医大学)第二附属医院急诊部
- 作者简介：
[ 尚伟龙，E-mail: shangwl0414@163.com SHANG Weilong, E-mail: shangwl0414@163.com
[ 周人杰，E-mail: zhou_rj@aliyun.com ZHOU Renjie, E-mail: zhou_rj@aliyun.com
- 基金信息：国家自然科学基金青年科学基金(81701967)
DOI：10.16016/j.1000-5404.202002210
中图分类号： R378.11;R742.7;R977.6收稿：2020-02-27，
修回：2020-05-27，
纸质出版：2020-08-30
- 稿件说明：
移动端阅览
郑颖, 杨裔, 彭华刚, 等. 脂蛋白SA2275抗体治疗金黄色葡萄球菌脑脓肿的实验初探[J]. 第三军医大学学报, 2020,42(16):1655-1662.
Ying ZHENG, Yi YANG, Huagang PENG, et al. Therapeutic effect of polyclonal lipoprotein SA2275 antibodies on
Staphylococcus aureus -induced brain abscess in mice[J]. Journal of Third Military Medical University, 2020, 42(16): 1655-1662.郑颖, 杨裔, 彭华刚, 等. 脂蛋白SA2275抗体治疗金黄色葡萄球菌脑脓肿的实验初探[J]. 第三军医大学学报, 2020,42(16):1655-1662. DOI： 10.16016/j.1000-5404.202002210.
Ying ZHENG, Yi YANG, Huagang PENG, et al. Therapeutic effect of polyclonal lipoprotein SA2275 antibodies on
Staphylococcus aureus -induced brain abscess in mice[J]. Journal of Third Military Medical University, 2020, 42(16): 1655-1662. DOI： 10.16016/j.1000-5404.202002210.
摘要
目的
2
探讨脂蛋白特异性抗体通过血脑屏障及其在抑制金黄色葡萄球菌脑脓肿形成中的作用。
方法
2
利用大肠埃希菌表达并纯化重组金葡菌脂蛋白SA2275，制备特异性小鼠多克隆抗体(anti-SA2275)。制作金葡菌感染脑脓肿模型，利用ELISA法检测经腹腔注射的anti-SA2275在小鼠脑组织中的滴度，评估抗体通过血脑屏障的能力；再利用梯度稀释细菌计数法检测脑脓肿中的细菌载量，对脑脓肿进行连续病理切片检测脑脓肿的大小，评价抗体anti-SA2275治疗金葡菌脑脓肿的效果。
结果
2
成功获得高纯度SA2275重组蛋白；免疫小鼠获得anti-SA2275，效价为1:51 200；anti-SA2275注射金葡菌脑脓肿模型小鼠后，在动物脑组织中检测到抗体的存在；治疗组小鼠脑脓肿大小和菌载量显著低于阴性对照组。
结论
2
在金葡菌感染小鼠所致的脑脓肿形成过程中，脂蛋白SA2275特异性抗体可以通过血脑屏障分布至脑组织，显著抑制脑脓肿的形成及细菌的定植，具有治疗金葡菌脑脓肿的应用前景。
Abstract
Objective
2
To investigate the ability of specific polyclonal antibodies against
Staphylococcus aureus
(
S. aureus
) lipoprotein SA2275 to penetrate the blood-brain barrier for inhibiting the formation of
S. aureus
-induced brain abscess in mice.
Methods
2
We immunized BALB/c mouse mice with purified recombinant
S. aureus
lipoprotein SA2275 expressed in
Escherichia coli
to obtain specific mouse polyclonal antibodies against SA2275 (anti-SA2275). In a C57BL/6 mouse model of brain abscess induced by injecting
S. aureus
strain Newman into the brain
we tested the titer of anti-SA2275 in the brain tissue using ELISA following intraperitoneal injection of the antibodies to evaluate the ability of the antibodies to pass through the blood-brain barrier. The bacterial load in the mouse brain was determined by gradient dilution bacterial counting
and the size of the brain abscess was evaluated on serial sections.
Results
2
The recombinant SA2275 protein with a high purity was successfully obtained. The results of ELISA showed that immunization of the mice with the recombinant SA2275 protein resulted in a high titer of anti-SA2275 reaching 1:51 200. In the mouse models of
S. aureus
-induced brain abscess
the presence of the antibodies was detected in the brain tissue after intraperitoneal injection of anti-SA2275. Treatment with anti-SA2275
as compared with blank control mouse serum
significantly reduced the size of the brain abscess and lowered the bacterial load in the brain tissues of the mice.
Conclusion
2
In mouse models of
S. aureus
-induced brain abscess
intraperitoneally injected lipoprotein SA2275-specific antibodies can penetrate the blood-brain barrier to reach the brain tissue and inhibit the formation of brain abscess and bacterial colonization
suggesting the potential of the antibodies for treatment of
S. aureus
-induced brain abscess.
关键词
Keywords
references
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