Rasmussen脑炎患者脑组织的转录组学特征分析

罗乔荔; 王一松; 关宇光; 陈思畅; 范东瀛; 王培刚; 栾国明; 安静

doi:10.16016/j.1000-5404.202002076

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Rasmussen脑炎患者脑组织的转录组学特征分析

神经科学 | 更新时间：2026-01-09

- Rasmussen脑炎患者脑组织的转录组学特征分析
- Analysis of transcriptomic characteristics of brain tissue in patients with Rasmussen encephalitis
- 第三军医大学学报 2020年42卷第14期页码：1407-1413
- 作者机构：
  
  1.100069 北京，首都医科大学基础医学院病原生物学系微生物学教研室
  2.100093 北京，首都医科大学三博脑科医院功能神经外科
- 作者简介：
  
  [栾国明, E-mail:luangm3@163.comAN Jing, E-mail: anjing@ccmu.edu.cn
  [安静, E-mail:anjing@ccmu.edu.cnLUAN Guoming, E-mail: luangm3@163.com
- 基金信息：
  
  国家自然科学基金青年基金项目(81701992)
- DOI：10.16016/j.1000-5404.202002076
  中图分类号： R322.81;R394.3;R725.123
- 收稿：2020-02-13，
  
  修回：2020-03-13，
  
  纸质出版：2020-07-30
- 稿件说明：
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罗乔荔, 王一松, 关宇光, 等. Rasmussen脑炎患者脑组织的转录组学特征分析[J]. 第三军医大学学报, 2020,42(14):1407-1413.

Qiaoli LUO, Yisong WANG, Yuguang GUAN, et al. Analysis of transcriptomic characteristics of brain tissue in patients with Rasmussen encephalitis[J]. Journal of Third Military Medical University, 2020, 42(14): 1407-1413.
罗乔荔, 王一松, 关宇光, 等. Rasmussen脑炎患者脑组织的转录组学特征分析[J]. 第三军医大学学报, 2020,42(14):1407-1413. DOI： 10.16016/j.1000-5404.202002076.

Qiaoli LUO, Yisong WANG, Yuguang GUAN, et al. Analysis of transcriptomic characteristics of brain tissue in patients with Rasmussen encephalitis[J]. Journal of Third Military Medical University, 2020, 42(14): 1407-1413. DOI： 10.16016/j.1000-5404.202002076.

摘要

目的

探究Rasmussen脑炎(Rasmussen encephalitis，RE)患者脑组织的转录组学特征。

方法

采用RNA-seq的方法，对2008年4月至2013年12月首都医科大学附属北京三博脑科医院收治的RE及颞叶癫痫(temporal lobe epilepsy，TLE)患者(对照)中各选取4例脑组织的基因表达谱进行检测。

结果

聚类分析显示RE患者及对照脑组织差异表达基因(differentially expressed genes，DEGs)谱存在明显差异。共筛选出1 285个DEGs，其中782个(60.9%)下调，503个(39.1%)上调。上调表达的DEGs主要富集于神经元信号转导相关的通路，可能与RE患者频繁的癫痫发作相关；下调表达的DEGs主要富集于细胞骨架、细胞周期及趋化作用相关的通路，可能与RE患者脑组织中神经元的丢失及再生以及部分病原体感染后的免疫应答相关。上调表达的关键基因为TAS2R40、HTR4、NAP1L2，下调表达的关键基因为CXXC1、SEPTIN8、CCR7。

结论

RE患者脑组织的转录组学具有明显特征，DEGs主要富集于神经元信号转导、细胞周期以及趋化作用相关的通路中。

Abstract

Objective

To investigate the transcriptomic characteristics of the brain tissue in patients with Rasmussen encephalitis (RE).

Methods

RNA-seq technique was used to detect the gene expression profiles in the brain tissues from 4 patients with RE and 4 with temporal lobe epilepsy (TLE) admitted to Sanbo Brain Hospital

Capital Medical University between April

2008 and December

2013.

Results

Cluster analysis showed significant differences in the differentially expressed genes (DEGs) in the brain tissue between the patients with RE and those with TLE. We identified a total of 1 285 DEGs

of which 782 (60.9%) were down-regulated and 503 (39.1%) were up-regulated in RE. The up-regulated DEGs were mainly enriched in the pathways associated with neuronal signal transduction

possibly in relation with frequent seizures in patients with RE; the down-regulated DEGs were mainly enriched in the pathways associated with cytoskeleton

cell cycle and chemotaxis

which may be related to neuronal loss and regeneration and immune responses to infection by some pathogens in the brain tissue of patients with RE. TAS2R40

HTR4 and NAP1L2 were identified as the key up-regulated DEGs

and CXXC1

SEPTIN8 and CCR7 as the key down-regulated DEGs.

Conclusion

The brain tissue of patients with RE shows characteristic transcriptomic changes

and the DEGs are mainly enriched in neuronal signal transduction

cell cycle and chemotaxis-related pathways.

关键词

Keywords

references

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