Heregulin-β1诱导硬脂酰辅酶A去饱和酶1促进乳腺癌细胞的增殖、迁移和侵袭

陈璟若; 马雪娇; 吕思楠; 安琪尔; 赵玉华

doi:10.16016/j.1000-5404.202001179

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Heregulin-β1诱导硬脂酰辅酶A去饱和酶1促进乳腺癌细胞的增殖、迁移和侵袭

基础医学 | 更新时间：2026-01-09

- Heregulin-β1诱导硬脂酰辅酶A去饱和酶1促进乳腺癌细胞的增殖、迁移和侵袭
- Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1
- 第三军医大学学报 2020年42卷第11期页码：1091-1100
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202001179
  中图分类号：
- 收稿：2020-01-20，
  
  纸质出版：2020-06-15
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陈璟若, 马雪娇, 吕思楠, 等. Heregulin-β1诱导硬脂酰辅酶A去饱和酶1促进乳腺癌细胞的增殖、迁移和侵袭[J]. 第三军医大学学报, 2020,42(11):1091-1100.

Jingruo CHEN, Xuejiao MA, Sinan LYU, et al. Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1[J]. Journal of Third Military Medical University, 2020, 42(11): 1091-1100.
陈璟若, 马雪娇, 吕思楠, 等. Heregulin-β1诱导硬脂酰辅酶A去饱和酶1促进乳腺癌细胞的增殖、迁移和侵袭[J]. 第三军医大学学报, 2020,42(11):1091-1100. DOI： 10.16016/j.1000-5404.202001179.

Jingruo CHEN, Xuejiao MA, Sinan LYU, et al. Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1[J]. Journal of Third Military Medical University, 2020, 42(11): 1091-1100. DOI： 10.16016/j.1000-5404.202001179.

摘要

Abstract

Objective

To investigate the heregulin-β1 (HRG-β1)-induced expression of stearoyl-CoA desaturase-1 (SCD1) and the effect of its activity of SCD1 on the proliferation

migration and invasion of breast cancer cells MCF7 and SK-BR-3.

Methods

MCF7 and SK-BR-3 cells were treated with control (PBS) or HRG-β1 at different concentrations and for different times. Then RT-qPCR and Western blotting were used to detect the mRNA and protein expression levels of SCD1. The wound healing assay and Transwell assay were employed to measure the migration and invasion of MCF7 and SK-BR-3 cells treated with PBS

HRG-β1

A939572 and oleic acid (OA).

Results

HRG-β1 increased both SCD1 mRNA and protein levels in MCF7 and SK-BR-3 cells at a time-dose dependent manner. Compared with the control group

HRG-β1 promoted cell proliferation in MCF7 and SK-BR-3 cells (

0.01)

while proliferation rate was decreased after A939572 treatment (

0.01). Compared with the control group

HRG-β1 significantly increased the wound healing rates and invaded cell numbers of MCF7 and SK-BR-3 cells (

0.01). The wound healing rates and invaded cell numbers of both cell lines were significantly decreased after HRG-β1+A939572 treatment (

0.01). The addition of OA rescued the migration and invasion of both cell lines which were weakened by A939572 (

0.01).

Conclusions

HRG-β1 can up-regulate the expression of SCD1

and the protein partially mediates the effect of HRG-β1 in promoting the proliferation

migration and invasion in MCF7 and SK-BR-3 cells.

关键词

Keywords

references

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