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组织非特异性碱性磷酸酶与心肌梗死后心肌纤维化的相关性研究
Correlation between serum TNAP and myocardial fibrosis in patients after myocardial infarction
- 第三军医大学学报 2020年42卷第11期 页码：1072-1077
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(31800976);国家自然科学基金面上项目(81970203);中国心血管健康联盟心血管健康研究院“进阶”研究基金项目(2018-CCA-AF-034);中国心血管健康联盟心血管健康研究院“进阶”研究基金项目(ky-2017-07-08)
DOI：10.16016/j.1000-5404.201912297
中图分类号：收稿：2019-12-31，
纸质出版：2020-06-15
- 稿件说明：
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王立友, 雷韵祎, 高磊, 等. 组织非特异性碱性磷酸酶与心肌梗死后心肌纤维化的相关性研究[J]. 第三军医大学学报, 2020,42(11):1072-1077.
Liyou WANG, Yunyi LEI, Lei GAO, et al. Correlation between serum TNAP and myocardial fibrosis in patients after myocardial infarction[J]. Journal of Third Military Medical University, 2020, 42(11): 1072-1077.
王立友, 雷韵祎, 高磊, 等. 组织非特异性碱性磷酸酶与心肌梗死后心肌纤维化的相关性研究[J]. 第三军医大学学报, 2020,42(11):1072-1077. DOI： 10.16016/j.1000-5404.201912297.
Liyou WANG, Yunyi LEI, Lei GAO, et al. Correlation between serum TNAP and myocardial fibrosis in patients after myocardial infarction[J]. Journal of Third Military Medical University, 2020, 42(11): 1072-1077. DOI： 10.16016/j.1000-5404.201912297.
摘要
Abstract
Objective
2
To investigate the relationship of tissue non-specific alkaline phosphatase (TNAP) with myocardial fibrosis in patients after myocardial infarction (MI).
Methods
2
Forty-six acute MI patients admitted to our hospital from March to November 2019 were enrolled in this study and assigned as the MI group
and another 41 patients with negative findings in coronary angiograms during the same period were collected as the control group. The serum TNAP activity was detected by alkaline phosphatase kit. The serum contents of procollagen type ⅠC-peptide (PⅠCP) and procollagen Ⅲ N-terminal peptide (PⅢNP) were detected by enzyme-linked immunosorbent assay (ELISA). Myocardial tissues from 5 patients who died of MI were collected. Collagen fiber area of heart was detected by Sirius red staining and the expression of TNAP was detected by immunohistochemistry. Ten 8-week-old male C57BL/6J mice were randomly divided into sham group and MI group (
n
=5). In 2 weeks after MI
all mice were sacrificed
and collagen fiber area of heart was detected by Masson staining and the expression of TNAP was detected by immunohistochemistry.
Results
2
Compared with the control group
the MI group had significantly increased serum TNAP activity (8.49±3.30 King U/100 mL
P
<
0.01)
higher serum contents of P Ⅰ CP and PⅢNP (both
P
<
0.01)
and the serum TNAP activity was statistically correlated with the serum contents of P Ⅰ CP and PⅢNP (
r
=0.38
P
<
0.01;
r
=0.40
P
<
0.01). There were more collagen deposition and higher TNAP expression level in the infarct border area of remote MI lesion than in the distal infarct (both
P
<
0.01). Compared with the mice of the sham group
the expression of TNAP and collagen fiber area in MI group were increased significantly (both
P
<
0.01)
and the expression was consistent with the distribution of fibers in the MI group.
Conclusions
2
TNAP is involved in the pathophysiological process of myocardial fibrosis after MI.
关键词
Keywords
references
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