不同吸氧时间对心脏骤停大鼠复苏后神经功能的影响

沈一鸣; 王秋红; 王建杰; 李永勤; 张丹

doi:10.16016/j.1000-5404.201912232

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不同吸氧时间对心脏骤停大鼠复苏后神经功能的影响

基础医学 | 更新时间：2026-01-09

- 不同吸氧时间对心脏骤停大鼠复苏后神经功能的影响
- Effect of different durations of post-resuscitation oxygen ventilation on neurological function in rats after cardiac arrest
- 第三军医大学学报 2020年42卷第11期页码：1078-1085
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市卫计委重点项目(2016ZDXM001);国家自然科学基金面上项目(81372102)
- DOI：10.16016/j.1000-5404.201912232
  中图分类号：
- 收稿：2019-12-24，
  
  纸质出版：2020-06-15
- 稿件说明：
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沈一鸣, 王秋红, 王建杰, 等. 不同吸氧时间对心脏骤停大鼠复苏后神经功能的影响[J]. 第三军医大学学报, 2020,42(11):1078-1085.

Yiming1 SHEN, Qiuhong2 WANG, Jianjie WANG, et al. Effect of different durations of post-resuscitation oxygen ventilation on neurological function in rats after cardiac arrest[J]. Journal of Third Military Medical University, 2020, 42(11): 1078-1085.
沈一鸣, 王秋红, 王建杰, 等. 不同吸氧时间对心脏骤停大鼠复苏后神经功能的影响[J]. 第三军医大学学报, 2020,42(11):1078-1085. DOI： 10.16016/j.1000-5404.201912232.

Yiming1 SHEN, Qiuhong2 WANG, Jianjie WANG, et al. Effect of different durations of post-resuscitation oxygen ventilation on neurological function in rats after cardiac arrest[J]. Journal of Third Military Medical University, 2020, 42(11): 1078-1085. DOI： 10.16016/j.1000-5404.201912232.

摘要

Abstract

Objective

To investigate the effect of pure oxygen ventilation under normothermia for different durations on the neurological outcome in

rat model of asphyxia cardiac arrest.

Methods

total of 32 male SD rats were treated with cardiopulmonary resuscitation 6 min after cardiac arrest induced by asphyxia. After return of spontaneous circulation (ROSC)

the rats were randomly divided into 4 groups according to the duration of ventilation of pure oxygen (8 in each group)

that is

0 min group (O

-0 min)

20 min group (O

-20 min)

60 min group (O

-60 min) and 180 min group (O

-180 min). Physiological parameters

continuous electroencephalography (EEG) waveforms

and EEG power of each band during post-resuscitation were recorded and compared among the groups. The serum contents of cardiac troponin

(cTnT) and S100 β protein were measured by enzyme-linked immunosorbent assay (ELISA) at the basic state and 6

after resuscitation. The neurological deficit score (NDS)

96-hour survival rate and histological changes of hippocampus were compared in the groups.

Results

After resuscitation

the mean arterial pressure (MAP) was decreased significantly

and the left ventricular ejection fraction (LVEF) was decreased first and then increased in the 4 groups. The 2 indicators were significantly higher in the O

-180 min group than the O

-0 min group (

0.05). With the increase of oxygen ventilation

shorter EEG recovery time and gradually stronger EEG power spectrum energy were observed in all the groups. The O

-180 min group had significantly shorter time and stronger energy than the O

-0 min group (

0.05). At 6

after resuscitation

cerebral and myocardial injuries were obviously milder in the O

-180 min group than the O

-0 min group (S100β

0.01; cTnT

0.05). NDS

survival rate and hippocampal injury were significantly improved in the O

-180 min group when compared with the O

-0 min group.

Conclusions

In this rat model of asphyxia cardiac arrest

prolonged pure oxygen inhalation under normothermia can alleviate cerebral injury

improve neurological outcome and increase the survival rate.

关键词

Keywords

references

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