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类风湿关节炎患者舌苔菌群微生态研究
Microecology of tongue coating flora in patients with rheumatoid arthritis
- 第三军医大学学报 2020年42卷第7期 页码：708-713
- 作者机构：
400038 重庆，陆军军医大学(第三军医大学)第一附属医院中医与风湿免疫科
- 作者简介：
[ 方勇飞. E-mail:fangyongfei@qq.com FANG Yongfei, E-mail:fangyongfei@qq.com
- 基金信息：国家自然科学基金面上项目(81072921)
DOI：10.16016/j.1000-5404.201912166
中图分类号： R241.25;R446.5;R593.22收稿：2019-12-08，
修回：2020-02-07，
纸质出版：2020-04-15
- 稿件说明：
移动端阅览
毛超飞, 孙辉, 邹庆华, 等. 类风湿关节炎患者舌苔菌群微生态研究[J]. 第三军医大学学报, 2020,42(7):708-713.
Chaofei MAO, Hui SUN, Qinghua ZOU, et al. Microecology of tongue coating flora in patients with rheumatoid arthritis[J]. Journal of Third Military Medical University, 2020, 42(7): 708-713.
毛超飞, 孙辉, 邹庆华, 等. 类风湿关节炎患者舌苔菌群微生态研究[J]. 第三军医大学学报, 2020,42(7):708-713. DOI： 10.16016/j.1000-5404.201912166.
Chaofei MAO, Hui SUN, Qinghua ZOU, et al. Microecology of tongue coating flora in patients with rheumatoid arthritis[J]. Journal of Third Military Medical University, 2020, 42(7): 708-713. DOI： 10.16016/j.1000-5404.201912166.
摘要
目的
2
从舌苔菌群微生态上研究类风湿关节炎患者疾病进展与菌群的关系。
方法
2
使用口腔咽拭子刮取活动期(OP组)、缓解期(OR组)及健康对照组(OHC)各10例的舌苔，使用口腔拭子基因组DNA快速提取试剂盒提取总DNA，进行16S rRNA扩增并进行Illumina MiSeq高通量测序，对测序结果进行微生态分析。
结果
2
3组样本在菌群的多样性上差异无统计学意义(
P
>
0.05)；3组样本有着各自独有的菌属，其中OP组7个菌
属，OR组4个菌属，OHC组10个菌属为各组独有；3组样本的优势菌群组成相同，但是在菌群构成中差异具有统计学意义，主要在厚壁菌门、放线菌门、Episilonbacteraeota菌门、变形菌门、奈瑟菌属、放线菌属、弯曲杆菌属和卟啉单胞菌属上(
P
均
<
0.05)。进行Beta多样性分析，通过PCA分析以及层级聚类分析，OP组和OHC组能够很好地聚集到一起，差异具有统计学意义。
结论
2
类风湿关节炎患者舌苔菌群与健康人群存在差异，其中主要表现在放线菌属和弯曲杆菌属丰度增加，奈瑟菌属和卟啉单胞菌属丰度减少。
Abstract
Objective
2
To investigate the relationship between disease progression and microecology of tongue coating flora in patients with rheumatoid arthritis (RA).
Methods
2
A total of 30 tongue coating flora swabs were collected from 20 RA patients
including 10 in the active stage (OP group) and 10 in the remission stage (OR group)
and 10 healthy individuals (OHC group).The total DNA extracted by rapid oral swab genomic DNA extraction kit was amplified with 16S rRNA and sequenced with high throughput Illumina MiSeq. The results were analyzed by microecology.
Results
2
There was no statistical difference in the diversity of 3 groups of samples (
P
>
0.05). All the 3 groups of samples had their own unique genera
including 7 genera in OP group
4 genera in OR group
and 10 genera in OHC group; The samples from 3 groups had the same composition of dominant flora
but obvious difference was seen in the composition of flora
mainly in
p
_
Firmicutes
p
_
Actinobacteria
p
_
Episilonbacteraeota
p
_
Proteobacteria
g
_
Neisseria
g
_
Actinomycetes
g
_
Campylobacter
and
g
_
Porphyromonas
(all
P
<
0.05). Through PCA analysis and hierarchical cluster analysis
OP group and OHC group can gather together well with significant differences.
Conclusion
2
There are differences between the flora of tongue coating in RA patients and healthy individuals. The abundances of
Actinomycetes
and
Campylobacter
are increased
while those of
Neisseria
and
Porphyromonas
are decreased.
关键词
Keywords
references
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