骨与软组织肉瘤患者检测循环肿瘤细胞及HOXB7基因表达的临床意义

杨松巍; 王亮; 李虎进; 单东力; 王全; 陈飚

doi:10.16016/j.1000-5404.201912123

您当前的位置：

首页 >

文章列表页 >

骨与软组织肉瘤患者检测循环肿瘤细胞及HOXB7基因表达的临床意义

临床医学 | 更新时间：2026-01-09

- 骨与软组织肉瘤患者检测循环肿瘤细胞及HOXB7基因表达的临床意义
- Clinical significance of circulating tumor cells and their homeobox gene B7 expression in patients with bone and soft tissue sarcomas
- 第三军医大学学报 2020年42卷第10期页码：1047-1052
- 作者机构：
  
  400030 重庆，重庆大学附属肿瘤医院骨与软组织肿瘤科
- 作者简介：
  
  [陈飚，E-mail: chenbiao1179@sina.comCHEN Biao, E-mail: chenbiao1179@sina.com
- 基金信息：
  
  重庆市科卫联合医学项目(2018QNXM019)
- DOI：10.16016/j.1000-5404.201912123
  中图分类号： R730.23;R730.43;R738.1
- 收稿：2019-12-12，
  
  修回：2020-03-12，
  
  纸质出版：2020-05-30
- 稿件说明：
移动端阅览
杨松巍, 王亮, 李虎进, 等. 骨与软组织肉瘤患者检测循环肿瘤细胞及HOXB7基因表达的临床意义[J]. 第三军医大学学报, 2020,42(10):1047-1052.

Songwei YANG, Liang WANG, Hujin LI, et al. Clinical significance of circulating tumor cells and their homeobox gene B7 expression in patients with bone and soft tissue sarcomas[J]. Journal of Third Military Medical University, 2020, 42(10): 1047-1052.
杨松巍, 王亮, 李虎进, 等. 骨与软组织肉瘤患者检测循环肿瘤细胞及HOXB7基因表达的临床意义[J]. 第三军医大学学报, 2020,42(10):1047-1052. DOI： 10.16016/j.1000-5404.201912123.

Songwei YANG, Liang WANG, Hujin LI, et al. Clinical significance of circulating tumor cells and their homeobox gene B7 expression in patients with bone and soft tissue sarcomas[J]. Journal of Third Military Medical University, 2020, 42(10): 1047-1052. DOI： 10.16016/j.1000-5404.201912123.

摘要

目的

探讨骨与软组织肉瘤患者外周血中循环肿瘤细胞(circulating tumor cell，CTC)的数量、类型以及循环肿瘤细胞上同源盒基因B7(homeobox genes B7，HOXB7)表达的临床意义。

方法

回顾性分析本院2017年5月至2019年7月经病理确诊的骨与软组织肉瘤患者40例，患者确诊后均采用CanPatrol

富集技术检测循环肿瘤细胞，采用多重mRNA原位分析对循环肿瘤细胞进行分型鉴定，并使用RNA探针检测循环肿瘤细胞上HOXB7基因的表达情况。结合临床资料，分析CTC计数、类型及HOXB7基因表达与骨与软组织肿瘤的分期及转移之间的关系。

结果

40例患者中有37例在治疗前检测出CTC细胞，检测出的CTC分为上皮型、混合型和间质型。CTC计数与骨与软组织肉瘤的分期密切相关(

=0.002)，Ennecking Ⅲ期患者外周血CTC计数明显多于Ennecking Ⅱ期(

=0.040)；Ennecking Ⅲ期患者间质型CTC的计数也显著高于Ennecking Ⅱ期(

=0.001)。HOXB7在Ennecking Ⅲ期患者CTC中的阳性表达率为56.9%，显著高于其他分期(

=0.003)。对骨肉瘤进行亚组分析，Enneckin

g Ⅲ期患者CTC中HOXB7表达的阳性率显著高于Ennecking Ⅱ期(

=0.025)。

结论

骨与软组织肉瘤患者外周血循环肿瘤细胞细胞计数、分型及HOXB7基因的表达与肿瘤分期、转移密切相关。

Abstract

Objective

To investigate the clinical significance of the count and type of circulating tumor cell (CTC) and the expression of homeobox gene B7 (HOXB7) gene in the CTC in patients with bone and soft tissue sarcomas.

Methods

We retrospectively analyzed the data of 40 patients with newly diagnosed bone and soft tissue sarcomas from May

2017 to July

2019 at Chongqing University Cancer Hospital. After the diagnosis

all the patients were examined for peripheral blood CTC using the CanPatrol

System

and the isolated CTC was characterized using a multiple messenger RNA

in situ

analysis (MRIA). The expression of HOXB7 gene in the CTC was detected using a RNA probe. The correlations of CTC counts

cell type and the expression level of HOXB7 gene with clinical stages and metastasis of bone and soft tissue sarcomas were analyzed.

Results

of the 40 patients with sarcoma

CTC including epithelial CTC

mesenchymal CTC (M-CTC)

and biophenotypic epithelial/mesenchymal CTC were detected in 37 patients before treatment. The CTC counts correlated significantly with Enneking stages (

=0.002). The number of CTC and the ratio of mesenchymal CTC were significantly greater in stage Ⅲ patients than in stage Ⅱ patients (

=0.040 and 0.001

respectively). Positive expression of HOXB7 in the CTC was detected in 56.9% of the stage Ⅲ patients

a rate significantly higher than that in patients in other stages (

=0.003). Subgroup analysis of osteosarcoma showed that the positivity rate of HOXB7 in the CDC was significantly higher in stage Ⅲ patients than in stage Ⅱ patients (

=0.025).

Conclusion

In patients with bone and soft tissue sarcomas

the counts and types of peripheral blood CTC and HOXB7 expression in the CDC are closely correlated with metastasis and clinical Enneking stages

and can serve as indicators for assessing tumor metastasis

therapeutic effect and prognosis of the patients.

关键词

Keywords

references

CHANG L, ASATRIAN G, DRY S M, et al . Circulating tumor cells in sarcomas: a brief review[J ] . Med Oncol, 2015, 32(1): 430. DOI: 10.1007/s12032-014-0430-9.

MEYERS P A, SCHWARTZ C L, KRAILO M D, et al . Osteosarcoma: the addition of muramyl tripeptide to chemotherapy improves overall survival—a report from the Children's Oncology Group[J ] . J Clin Oncol, 2008, 26(4): 633-638. DOI: 10.1200/JCO.2008.14.0095.

KIM M Y, OSKARSSON T, ACHARYYA S, et al . Tumor self-seeding by circulating cancer cells[J ] . Cell, 2009, 139(7): 1315-1326. DOI: 10.1016/j.cell.2009.11.025.

ZHONG X, ZHANG H, ZHU Y, et al . Circulating tumor cells in cancer patients: developments and clinical applications for immunotherapy[J ] . Mol Cancer, 2020, 19(1): 15. DOI: 10.1186/s12943-020-1141-9.

PAOLETTI C, HAYES D F. Circulating tumor cells[J]. Adv Exp Med Biol, 2016, 882: 235-258. DOI: 10.1007/978-3-319-22909-6_10.

CABEL L, PROUDHON C, GORTAIS H, et al . Circulating tumor cells: clinical validity and utility[J ] . Int J Clin Oncol, 2017, 22(3): 421-430. DOI: 10.1007/s10147-017-1105-2.

WELLIK D M. Hox genes and vertebrate axial pattern[J]. Curr Top Dev Biol, 2009, 88: 257-278. DOI: 10.1016/S0070-2153(09)88009-5.

LIAO W T, JIANG D, YUAN J, et al . HOXB7 as a prognostic factor and mediator of colorectal cancer progression[J ] . Clin Cancer Res, 2011, 17(11): 3569-3578. DOI: 10.1158/1078-0432.CCR-10-2533.

原薇薇, 谭艳, 杜敏, 等. HOXB7基因在肺腺癌中的表达及其临床意义[J].临床肿瘤学杂志, 2014, 19(11): 987-991.

YUAN W W, TAN Y, DU M, et al . Expression of HOXB7 in human lung adenocarcinoma and its clinical significance[J ] . Chin Clin Oncol, 2014, 19(11): 987-991.

BITU C C, CARRERA M, LOPES M A, et al . HOXB7 expression is a prognostic factor for oral squamous cell carcinoma[J ] . Histopathology, 2012, 60(4): 662-665. DOI: 10.1111/j.1365-2559.2011.04102.x.

RUBIN E, WU X, ZHU T, et al . A role for the HOXB7 homeodomain protein in DNA repair[J ] . Cancer Res, 2007, 67(4): 1527-1535. DOI: 10.1158/0008-5472.CAN-06-4283.

WU S, LIU S, LIU Z, et al . Classification of circulating tumor cells by epithelial-mesenchymal transition markers[J ] . PLoS ONE, 2015, 10(4): e0123976. DOI: 10.1371/journal.pone.0123976.

LI M, LU Y, LONG Z, et al . Prognostic and clinicopathological significance of circulating tumor cells in osteosarcoma[J ] . J Bone Oncol, 2019, 16: 100236. DOI: 10.1016/j.jbo.2019.100236.

WU Z J, TAN J C, QIN X, et al . Significance of circulating tumor cells in osteosarcoma patients treated by neoadjuvant chemotherapy and surgery[J ] . Cancer Manag Res, 2018, 10: 3333-3339. DOI: 10.2147/CMAR.S176515.

GUPTA G P, MASSAGUE J. Cancer metastasis: building a framework[J]. Cell, 2006, 127(4): 679-695. DOI: 10.1016/j.cell.2006.11.001.

CHEN X X, BAI F. Single-cell analyses of circulating tumor cells[J]. Cancer Biol Med, 2015, 12(3): 184-192. DOI: 10.7497/j.issn.2095-3941.2015.0056.

YU M, BARDIA A, WITTNER B S, et al . Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition[J ] . Science, 2013, 339(6119): 580-584. DOI: 10.1126/science.1228522.

李明辉, 鲁亚杰, 张浩强, 等.骨肉瘤外周血循环肿瘤细胞检测的临床意义[J].中华骨科杂志, 2018, 38(6): 330-337. DOI: 10.3760/cma.j.issn.0253-2352.2018.06.002.

LI M H, LU Y J, ZHANG H Q, et al . Clinical significance of peripheral blood circulating tumor cells detection in osteosarcoma patients[J ] . Chin J Orthop, 2018, 38(6): 330-337. DOI: 10.3760/cma.j.issn.0253-2352.2018.06.002.

THIERY J P, ACLOQUE H, HUANG R Y, et al . Epithelial-mesenchymal transitions in development and disease[J ] . Cell, 2009, 139(5): 871-890. DOI: 10.1016/j.cell.2009.11.007.

KANG Y, PANTEL K. Tumor cell dissemination: emerging biological insights from animal models and cancer patients[J]. Cancer Cell, 2013, 23(5): 573-581. DOI: 10.1016/j.ccr.2013.04.017.

WU X, CHEN H, PARKER B, et al . HOXB7, a homeodomain protein, is overexpressed in breast cancer and confers epithelial-mesenchymal transition[J ] . Cancer Res, 2006, 66(19): 9527-9534. DOI: 10.1158/0008-5472.CAN-05-4470.

YANG Y, CHEN J, CHEN Q. Upregulation of HOXB7 promotes proliferation and metastasis of osteosarcoma cells[J]. Mol Med Rep, 2017, 16(3): 2773-2778. DOI: 10.3892/mmr.2017.6906.

YANG L, XIE F, LI S. Downregulation of Homeobox B7 inhibits the tumorigenesis and progression of osteosarcoma[J]. Oncol Res, 2017, 25(7): 1089-1095. DOI: 10.3727/096504016X14784668796788.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

乳腺癌循环肿瘤细胞与原发肿瘤临床病理特征关系的初步研究