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黑色素瘤细胞外泌体对成纤维细胞侵袭和MMP2、MMP9表达的影响
Effects of melanoma cell exosomes on fibroblast invasion and expression of MMP2 and MMP9
in vitro - 第三军医大学学报 2020年42卷第8期 页码：822-829
- 作者机构：
- 作者简介：
- 基金信息：
DOI：10.16016/j.1000-5404.201912001
中图分类号：收稿：2019-12-01，
纸质出版：2020-04-30
- 稿件说明：
移动端阅览
李豪, 马雪辉, 段乳侠, 等. 黑色素瘤细胞外泌体对成纤维细胞侵袭和MMP2、MMP9表达的影响[J]. 第三军医大学学报, 2020,42(8):822-829.
Hao LI, Xuehui MA, Ruxia DUAN, et al. Effects of melanoma cell exosomes on fibroblast invasion and expression of MMP2 and MMP9
in vitro [J]. Journal of Third Military Medical University, 2020, 42(8): 822-829.李豪, 马雪辉, 段乳侠, 等. 黑色素瘤细胞外泌体对成纤维细胞侵袭和MMP2、MMP9表达的影响[J]. 第三军医大学学报, 2020,42(8):822-829. DOI： 10.16016/j.1000-5404.201912001.
Hao LI, Xuehui MA, Ruxia DUAN, et al. Effects of melanoma cell exosomes on fibroblast invasion and expression of MMP2 and MMP9
in vitro [J]. Journal of Third Military Medical University, 2020, 42(8): 822-829. DOI： 10.16016/j.1000-5404.201912001.
摘要
Abstract
Objective
2
To investigate the effects of melanoma exosomes on fibroblast invasion and MMP2 and MMP9 expression.
Methods
2
The extracellular bodies of melanoma were extracted by ultracentrifugation. The surface markers and morphology of exosomes were identified by Western blot assay
transmission electron microscopy and particle size detection. Exosome-treated fitroblasts were observed by flow cytometry and laser confocal microscopy. After the process of ingestion
Transwell assay was used to detect the effect of melanoma exosomes on the invasive ability of fibroblasts. Western blot assay and immunocytochemistry were used to detect the effect of exosomes on the expression of MMP2 and MMP9 in fibroblasts. The effect of MMP2 and MMP9 gene levels was examined using qRT-PCR.
Results
2
The morphology of melanoma exosomes was observed as double-layered membrane
hollow
round or elliptical
and positively expressed exosome marker TSG101 and melanoma marker protein TYRP2. Compared with normal fibroblasts
the invasive ability of melanoma exosomes treated fibroblasts was significantly increased
and MMP2 and MMP9 expression were significantly up-regulated.
Conclusion
2
Melanoma exosomes may promote fibroblast invasion by inducing the expression of MMP2 and MMP9 in fibroblasts.
关键词
Keywords
references
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