基于酞菁铁的靶向乳腺癌纳米粒多模态成像和光热效应的实验研究

何雨蓓; 郝兰; 李倩茹; 黄美盈; 熊亚岚; 郑晨曦; 王志刚; 袁耿彪

doi:10.16016/j.1000-5404.201911260

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基于酞菁铁的靶向乳腺癌纳米粒多模态成像和光热效应的实验研究

基础医学 | 更新时间：2026-01-09

- 基于酞菁铁的靶向乳腺癌纳米粒多模态成像和光热效应的实验研究
- Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer
- 第三军医大学学报 2020年42卷第8期页码：772-782
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市科技局基础研究与前沿探索(CSTC2018jcyjAX0747);国家自然科学基金重点项目(31630026)
- DOI：10.16016/j.1000-5404.201911260
  中图分类号：
- 收稿：2019-11-30，
  
  纸质出版：2020-04-30
- 稿件说明：
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何雨蓓, 郝兰, 李倩茹, 等. 基于酞菁铁的靶向乳腺癌纳米粒多模态成像和光热效应的实验研究[J]. 第三军医大学学报, 2020,42(8):772-782.

Yubei HE, Lan HAO, Qianru LI, et al. Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer[J]. Journal of Third Military Medical University, 2020, 42(8): 772-782.
何雨蓓, 郝兰, 李倩茹, 等. 基于酞菁铁的靶向乳腺癌纳米粒多模态成像和光热效应的实验研究[J]. 第三军医大学学报, 2020,42(8):772-782. DOI： 10.16016/j.1000-5404.201911260.

Yubei HE, Lan HAO, Qianru LI, et al. Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer[J]. Journal of Third Military Medical University, 2020, 42(8): 772-782. DOI： 10.16016/j.1000-5404.201911260.

摘要

Abstract

Objective

To prepare a novel nanoparticle carrying iron(Ⅱ) phthalocyanine (FePc) coupled with aptamer AS1411 for targeting human breast cancer MCF-7 cells and evaluate its physicochemical properties

targeting ability to MCF-7 cells

photoacoustic/ultrasonic multimodal imaging capabilities

photothermal performance

photoinduced phase transitions

in vitro

and multimodal imaging performance

in vivo

Methods

FePc-encapsulated non-targeted nanoparticles (PLGA@FePc@PFP) were prepared by a two-step phacoemulsification method

and the targeted nanoparticles (AS1411-PLGA@FePc@PFP NPs) were prepared by combining the aptamer AS1411 modified with NH

and the PLGA@FePc@PFP nanoparticles using the carbodiimide method. The physicochemical properties of AS1411-PLGA@FePc@PFP NPs were detected

and laser confocal microscopy and flow cytometry were used to evaluate the binding between AS1411 and the nanoparticles. CCK-8 assay was used to detect the cytotoxicity of AS1411-PLGA@FePc@PFP; laser confocal microscopy was used to observe the

in vitro

targeting ability of the nanoparticles; photoacoustic and ultrasonic imagers were used to test their imaging performance and targeting ability both

in vitro

and

in vivo

. Near-i

nfrared laser (660 nm) and thermal imagers were used to test the

in vitro

photothermal conversion abilities of the nanoparticles

and the changes of the nanoparticles after laser irradiation were observed under an optical microscope. The temperature change of AS1411-PLGA@FePc@ PFP in tumor-bearing nude mice was detected in response to 660 nm laser irradiation.

Results

AS1411-PLGA@FePc@PFP was successfully prepared

which had an average diameter of 208.03±8.96 nm with a narrow particle size distribution

a mean zeta potential of -10.8±1.14 mV

and a FePc encapsulation rate of (83.96±1.61)%. The results of CCK-8 assay showed that the nanoparticles at varying concentrations had no obvious cytotoxicity. Flow cytometry showed an efficient binding of AS1411 with the nanoparticles had a binding rate of (93.31±2.58)%. Laser confocal analysis showed that there were significantly more nanoparticles around MCF-7 cells in the targeted group than in the non-targeted group. Laser irradiation caused the temperature of the nanoparticles carrying FePc to increase rapidly with time

and the phase change of the nanoparticles was observed under an optical microscope

suggesting good photothermal conversion ability of the nanoparticles. Photoacoustic imaging results showed that compared with nanoparticles without FePc

AS1411-PLGA@FePc@PFP produced strong photoacoustic signals

and the signal intensity showed a positive linear correlation with the nanoparticle concentration. Ultrasound and imaging results showed FePc in AS1411-PLGA@FePc@PFP achieved good photothermal conversion in a concentration-dependent manner for enhancing the effect of ultrasound imaging. In the tumor-bearing nude mice

the photoacoustic signal values in the xenograft were 1.26±0.06 and 0.30±0.01 at 6 h after tail vein injection of AS1411-PLGA@FePc@PFP and PLGA@FePc@PFP

respectively

showing a significant difference in their signal intensity (

0.05);

in vivo

ultrasound/contr

ast imaging also demonstrated a significant difference in the signal value after laser irradiation between the targeted group and the non-targeted group (

0.05). After laser irradiation

the tumor temperature in the targeted nanoparticle group was 50.53±0.56 ℃

as compared with 40.63±0.78 ℃ in the non-targeted group.

Conclusion

A multimodal molecular probe AS1411-PLGA@FePc@PFP for breast cancer has been successfully prepared

which has a good photothermal effect and can be potentially used for photoacoustic/ultrasound multimodal imaging of breast cancer.

关键词

Keywords

references

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