血小板微囊泡对人微血管内皮细胞功能的影响

潘丽娜; 汪泽扬; 陈丽媛; 张润军; 肇炜博; 胡厚源

doi:10.16016/j.1000-5404.201911253

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血小板微囊泡对人微血管内皮细胞功能的影响

基础医学 | 更新时间：2026-01-09

- 血小板微囊泡对人微血管内皮细胞功能的影响
- Effect of platelet microvesicles on the function of human microvascular endothelial cells
- 第三军医大学学报 2020年42卷第8期页码：758-764
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81470561);国家自然科学基金面上项目(81770433)
- DOI：10.16016/j.1000-5404.201911253
  中图分类号：
- 收稿：2019-11-29，
  
  纸质出版：2020-04-30
- 稿件说明：
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潘丽娜, 汪泽扬, 陈丽媛, 等. 血小板微囊泡对人微血管内皮细胞功能的影响[J]. 第三军医大学学报, 2020,42(8):758-764.

Lina PAN, Zeyang WANG, Liyuan CHEN, et al. Effect of platelet microvesicles on the function of human microvascular endothelial cells[J]. Journal of Third Military Medical University, 2020, 42(8): 758-764.
潘丽娜, 汪泽扬, 陈丽媛, 等. 血小板微囊泡对人微血管内皮细胞功能的影响[J]. 第三军医大学学报, 2020,42(8):758-764. DOI： 10.16016/j.1000-5404.201911253.

Lina PAN, Zeyang WANG, Liyuan CHEN, et al. Effect of platelet microvesicles on the function of human microvascular endothelial cells[J]. Journal of Third Military Medical University, 2020, 42(8): 758-764. DOI： 10.16016/j.1000-5404.201911253.

摘要

Abstract

Objective

To investigate the effect of platelet microvesicles (PMVs) on the function of human microvascular endothelial cells (HMECs).

Methods

Blood samples from healthy volunteers were collected and anticoagulated with one-tenth volume of citrate solution to isolate platelet-rich plasma (PRP). PMVs released from adenosine diphosphate (ADP)-activated human platelets was collected by centrifugation. HMECs were cultured and grouped as following: Tyrode's buffer as control (Con) group

final concentration of PMVs at 10

50 μg/mL respectively

and supernatant (Sup) of PMVs (almost PMVs free) for 24 h. ELISA was used to detect the contents of thromboxane B2 (TXB2) and 6-keto-PGF1α

the stable metabolites of TXA2 and prostaglandin I2 (PGI2) respectively

intercellular adhesion molecule-1 (ICAM-1)

vascular cell adhesion molecule-1 (VCAM-1) and E-selectin in the culture supernatant of HMECs. The expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) phosphorylation in HMECs were determined by Western blotting. Nitric oxide (NO) production in HMECs was measured by fluorescent probe. Endothelial microvesicles (EMVs) secreted by HMECs in the culture supernatant were determined by flow cytometry.

Results

Compared with Con group and Sup group

PMVs significantly upregulated the level of TXB2 and decreased that of 6-keto-PGF1α in the supernatant of HMECs in a time- and dose-dependent manner (

0.05). After incubation with PMVs

the phosphorylated eNOS and the production of NO in HMECs were reduced

while the expression levels of ET-1

ICAM-1

VCAM-1 and E-selectin in the supernatant of HMECs were increased significantly (

0.05). The concentration of EMVs induced by 50 μg/mL PMVs for 24 h was were increased obviously when compared with con group and Sup group (

0.05).

Conclusion

PMVs may induce the to dysfunction and damage of microvascular endothelial cells. This might be one of the important mechanisms by which platelets are involved in microcirculation dysfunction.

关键词

Keywords

references

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