右美托咪定调节巨噬细胞极化抑制肾缺血再灌注系统炎性反应

何心海; 陈星同; 杨子恒; 古丽; 周莉; 全俊先; 鲁开智; 顾健腾

doi:10.16016/j.1000-5404.201911107

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右美托咪定调节巨噬细胞极化抑制肾缺血再灌注系统炎性反应

基础医学 | 更新时间：2026-01-09

- 右美托咪定调节巨噬细胞极化抑制肾缺血再灌注系统炎性反应
- Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization
- 第三军医大学学报 2020年42卷第6期页码：611-615
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81772050);重庆市基础和前沿研究基金(cstc2018jcyjAX0577)
- DOI：10.16016/j.1000-5404.201911107
  中图分类号：
- 收稿：2019-11-13，
  
  纸质出版：2020-03-30
- 稿件说明：
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何心海, 陈星同, 杨子恒, 等. 右美托咪定调节巨噬细胞极化抑制肾缺血再灌注系统炎性反应[J]. 第三军医大学学报, 2020,42(6):611-615.

Xinhai HE, Xintong CHEN, Ziheng YANG, et al. Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization[J]. Journal of Third Military Medical University, 2020, 42(6): 611-615.
何心海, 陈星同, 杨子恒, 等. 右美托咪定调节巨噬细胞极化抑制肾缺血再灌注系统炎性反应[J]. 第三军医大学学报, 2020,42(6):611-615. DOI： 10.16016/j.1000-5404.201911107.

Xinhai HE, Xintong CHEN, Ziheng YANG, et al. Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization[J]. Journal of Third Military Medical University, 2020, 42(6): 611-615. DOI： 10.16016/j.1000-5404.201911107.

摘要

Abstract

Objective

To explore the effects of dexmedetomidine (Dex) on systemic inflammatory response induced by renal ischemia reperfusion (rIRI) and the possible mechanism.

Methods

Fifteen C57BL/6J mice were randomly divided into 3 groups

that is

sham operation group (laparotomy without renal pedicle occlusion)

rIRI group (bilateral renal pedicles was clamped for 60 min with a microvascular clamp)

and rIRI+Dex group (intraperitoneal injection of 25 μg/ml Dex in 15 min prior to bilateral renal ischemia). In 24 h after surgery or reperfusion

the blood samples were harvested and analyzed for counts of neutrophils

monocytes and lymphocytes by blood analyzer and for the serum levels of inflammatory cytokines (TNF-α

IL-1β and IL-10) by ELISA. Western blotting was used to detect iNOS and Arg 1 in the harvested peritoneal macrophages for the polarization.

Results

Compared with the mice in the sham group

the counts of neutrophils

monocytes and lymphocytes were significantly elevated (

0.01)

the serum levels of TNF-α and IL-1β were increased (

0.01)

and the expression of M1 marker iNOS was enhanced while that of M2 marker Arg 1 was decreased (

0.01). Dex pretreatment resulted in no significant changes in the counts of neutrophils

monocytes and lymphocytes (

0.05)

reduced serum levels of TNF-α and IL-1β (

0.01) and increased level of IL-10 (

0.01)

and decreased expression of iNOS (

0.01) as well as increase of Arg 1 (

0.05) when compared with the indicators in the rIRI group.

Conclusion

Dex inhibits the systemic inflammatory response induced by rIRI

which may be associated with modulation of macrophage polarization.

关键词

Keywords

references

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