多肽bCAT联合卡泊芬净对白色念珠菌菌丝及成熟生物被膜的作用

刘贤; 许珊; 沈一鸣; 亢胜男; 刘景仑; 陈晓迎; 张丹

doi:10.16016/j.1000-5404.201911065

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多肽bCAT联合卡泊芬净对白色念珠菌菌丝及成熟生物被膜的作用

基础医学 | 更新时间：2026-01-09

- 多肽bCAT联合卡泊芬净对白色念珠菌菌丝及成熟生物被膜的作用
- Effect of polypeptide bCAT combined with caspofungin on hypha and mature biofilm in Candida albicans
- 第三军医大学学报 2020年42卷第7期页码：671-677
- 作者机构：
  
  400016，重庆，重庆医科大学附属第一医院急诊医学科
- 作者简介：
  
  [张丹，E-mail: doctor_zhangdan@126.com ZHANG Dan, E-mail: doctor_zhangdan@126.com
- 基金信息：
  
  重庆市卫计委重点项目(2016ZDXM001);国家自然科学基金面上项目(81071531);国家自然科学基金面上项目(81372102)
- DOI：10.16016/j.1000-5404.201911065
  中图分类号： R379.4;R977.6;R978.5
- 收稿：2019-11-08，
  
  修回：2020-01-03，
  
  纸质出版：2020-04-15
- 稿件说明：
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刘贤, 许珊, 沈一鸣, 等. 多肽bCAT联合卡泊芬净对白色念珠菌菌丝及成熟生物被膜的作用[J]. 第三军医大学学报, 2020,42(7):671-677.

Xian LIU, Shan XU, Yiming SHEN, et al. Effect of polypeptide bCAT combined with caspofungin on hypha and mature biofilm in Candida albicans[J]. Journal of Third Military Medical University, 2020, 42(7): 671-677.
刘贤, 许珊, 沈一鸣, 等. 多肽bCAT联合卡泊芬净对白色念珠菌菌丝及成熟生物被膜的作用[J]. 第三军医大学学报, 2020,42(7):671-677. DOI： 10.16016/j.1000-5404.201911065.

Xian LIU, Shan XU, Yiming SHEN, et al. Effect of polypeptide bCAT combined with caspofungin on hypha and mature biofilm in Candida albicans[J]. Journal of Third Military Medical University, 2020, 42(7): 671-677. DOI： 10.16016/j.1000-5404.201911065.

摘要

目的

探讨嗜铬粒蛋白A(chromogranin

CGA)衍生多肽bCAT联合卡泊芬净(caspofungin

CAS)对白色念珠菌菌丝及成熟生物被膜的作用及机制。

方法

以标准菌株ATCC10231为研究对象，体外建立生物被膜模型，分别用bCAT、CAS、bCAT联合CAS作用于被膜，以RPMI1640干预作为空白对照组(control组)。XTT法检测生物被膜代谢活性的变化，并计算抗成熟生物被膜50%的MIC(MBIC

)，RT-PCR检测黏附因子ALS3的mRNA表达，DCFH-DA观察细

胞内ROS含量的变化，SYTO9/PI染色观察生物被膜通透性的变化。

结果

bCAT及CAS抗成熟生物被膜的MBIC

分别为3 105、128 μg/mL，两者联合使用时抗成熟被膜的MBIC

分别降至776、4 μg/mL，RT-PCR检测结果显示bCAT+CAS组处理被膜后ALS3表达量为(1.71±0.65)%，与control、bCAT(776 μg/mL)、CAS(4 μg/mL)组比较差异具有统计学意义(

0.05)；ROS含量为(428.96±47.67)%，与control、bCAT(776 μg/mL)、CAS(4 μg/mL)组比较差异具有统计学意义(

0.05)；bCAT+CAS组中死亡菌明显增加，菌丝相真菌明显减少。

结论

CGA衍生多肽bCAT联合CAS具有协同抗成熟被膜作用，其机制可能与降低黏附因子ALS3的表达而抑制菌丝的形成、增加ROS含量造成氧化损伤相关。

Abstract

Objective

To investigate the effect and mechanism of chromogranin (CGA)-derived polypeptide bCAT combined with caspofungin (CAS) on hypha and mature biofilm in

Candida albicans (C. albicans)

Methods

Standard strain ATCC 10231 of

C. albicans

was employed as the study objective. The changes of biofilm metabolic activity after bCAT and CAS treatment alone or combined together (RPMI1640 as control group) were detected by XTT assay

and the results were calculated for the minimal biofilm inhibitory concentration (MBIC

). The expression of agglutinin-like sequence 3 (ALS3) was detected by RT-PCR

and the production of reactive oxygen content (ROS) was observed by DCFH-DA. SYTO9/PI staining was used to detect the fungal membrane permeability.

Results

The MBIC

after bCAT and CAS treatment was 3 105 and 128 μg/mL

respectively. And the MBIC

of bCAT combined with CAS was decreased to 776 μg/mL and 4 μg/mL

respectively. The expression of ALS3 in the bCAT+CAS group was (1.71±0.65)%

which had statistical differences from that of the control

bCAT (776 μg/mL) and CAS (4 μg/mL) groups (

0.05). The ROS content was (428.96±47.67)%

with significant difference compared with the control

bCAT (776 μg/mL) and CAS (4 μg/mL) groups (

0.05). There were more dead

C. albicans

in mature biofilm in the bCAT+CAS group

and the fungal hypha were decreased significantly.

Conclusion

CGA-derived polypeptide bCAT combined with CAS has the synergistic anti-mature biofilm effect

and the mechanism may be associated with decreased expression of ALS3 and inhibition of hypha formation

and oxidative damage caused by increased ROS content.

关键词

Keywords

references

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