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携载AMD070的靶向超声纳米泡的构建及其抑制乳腺癌细胞生长的效能评估
Preparation of targeted nanobubbles carrying AMD070 and its inhibitory effect on growth of breast cancer cells
in vitro - 第三军医大学学报 2020年42卷第5期 页码：473-479
- 作者机构：
400038 重庆，陆军军医大学(第三军医大学)第一附属医院超声科
- 作者简介：
[郭燕丽，E-mail:guoyanli71@aliyun.comGUO Yanli, E-mail: guoyanli71@aliyun.com
- 基金信息：国家自然科学基金面上项目(81771856);陆军军医大学第一附属医院重大领域技术创新项目(SWH2016ZDCX4101)
DOI：10.16016/j.1000-5404.201911023
中图分类号： R445.1;R737.9;R944.9收稿：2019-11-04，
修回：2019-12-20，
纸质出版：2020-03-15
- 稿件说明：
移动端阅览
申戴佳, 朱连华, 刘玉, 等. 携载AMD070的靶向超声纳米泡的构建及其抑制乳腺癌细胞生长的效能评估[J]. 第三军医大学学报, 2020,42(5):473-479.
Daijia SHEN, Lianhua ZHU, Yu LIU, et al. Preparation of targeted nanobubbles carrying AMD070 and its inhibitory effect on growth of breast cancer cells
in vitro [J]. Journal of Third Military Medical University, 2020, 42(5): 473-479.申戴佳, 朱连华, 刘玉, 等. 携载AMD070的靶向超声纳米泡的构建及其抑制乳腺癌细胞生长的效能评估[J]. 第三军医大学学报, 2020,42(5):473-479. DOI： 10.16016/j.1000-5404.201911023.
Daijia SHEN, Lianhua ZHU, Yu LIU, et al. Preparation of targeted nanobubbles carrying AMD070 and its inhibitory effect on growth of breast cancer cells
in vitro [J]. Journal of Third Military Medical University, 2020, 42(5): 473-479. DOI： 10.16016/j.1000-5404.201911023.
摘要
目的
2
构建携载CXCR4小分子拮抗剂AMD070的诊疗一体化超声靶向纳米泡，观察其在体外与乳腺癌细胞的靶向结合能力，初步探讨靶向纳米泡联合超声靶向纳米微泡破坏(ultrasound targeted nano-microbubble destruction，UTMD)对乳腺癌细胞生长的抑制作用。
方法
2
通过碳二亚胺法和机械振荡法制备携载AMD070的靶向纳米泡，检测其物理特性，观察靶向纳米泡与乳腺癌细胞的特异性结合能力，CCK-8法筛选UTMD参数并评估在体外靶向纳米泡联合UTMD对乳腺癌细胞的生长抑制作用。
结果
2
携载AMD070的超声靶向纳米泡粒径(481.72±26.17)nm，电位(-7.14±0.31)mV，其中AMD070与脂质纳米泡有效连接，体外结合实验显示靶向纳米泡相较于空白纳米泡对CXCR4阳性乳腺癌细胞有更强的亲和力，靶向纳米泡联合优化的UTMD能明显抑制CXCR4阳性的乳腺癌细胞生长。
结论
2
靶向纳米泡能与CXCR4阳性表达的乳腺癌细胞特异性结合，并在优化后UTMD的配合下显著抑制CXCR4阳性乳腺癌细胞的生长。
Abstract
Objective
2
To prepare targeted ultrasonic nanobubbles carrying small-molecule CXCR4 antagonist AMD070
which can be used in integrated diagnosis and treatment
and investigate the binding ability to breast cancer cells
in vitro
in order to investigate the inhibitory effect of ultrasound targeted nanobubble destruction (UTMD) on the growth of breast cancer cells.
Methods
2
Targeted nanobubbles carrying AMD070 were prepared using the carbodiimide method and mechanical oscillation. Their particle size and zeta potential were assessed. The specific binding ability of targeted nanobubbles to breast cancer cells
in vitro
was evaluated. CCK-8 assay was used to optimize the parameters of UTMD and evaluate the inhibitory effect of targeted nanobubbles combined with UTMD on growth of breast cancer cells.
Result
2
The average particle size and zeta potential of ultrasonic targeted nanobubbles carrying AMD070 was 481.72±26.17 nm and -7.14±0.31 mV
respectively. AMD070 was efficiently bound to the nanobubbles.
In vitro
experiment showed that the targeted nanobubbles displayed stronger affinity to CXCR4 positive breast cancer cells when compared with the blank nanobubbles. When combined with UTMD
targeted nanobubbles exerted a remarkable inhibitory effect on the proliferation in CXCR4 positive breast cancer cells.
Conclusion
2
Targeted nanobubbles can specifically bind to CXCR4 positive breast cancer cells
and when combined with optimized UTMD
and the targeted nanobubbles can significantly inhibit the growth of CXCR4 positive breast cancer cells
in vitro
.
关键词
Keywords
references
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