胶原蛋白对去势SD大鼠萎缩阴道上皮作用探讨

游爽; 刘帅斌; 欧阳潇孏; 胡丽娜

doi:10.16016/j.1000-5404.201910187

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胶原蛋白对去势SD大鼠萎缩阴道上皮作用探讨

基础医学 | 更新时间：2026-01-09

- 胶原蛋白对去势SD大鼠萎缩阴道上皮作用探讨
- Therapeutic effect of collagen on vaginal atrophy in ovariectomized rats
- 第三军医大学学报 2020年42卷第6期页码：632-638，封三
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201910187
  中图分类号：
- 收稿：2019-10-28，
  
  纸质出版：2020-03-30
- 稿件说明：
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游爽, 刘帅斌, 欧阳潇孏, 等. 胶原蛋白对去势SD大鼠萎缩阴道上皮作用探讨[J]. 第三军医大学学报, 2020,42(6):632-638，封三.

Shuang YOU, Shuaibin LIU, Xiaolan OUYANG, et al. Therapeutic effect of collagen on vaginal atrophy in ovariectomized rats[J]. Journal of Third Military Medical University, 2020, 42(6): 632-638，封三.
游爽, 刘帅斌, 欧阳潇孏, 等. 胶原蛋白对去势SD大鼠萎缩阴道上皮作用探讨[J]. 第三军医大学学报, 2020,42(6):632-638，封三. DOI： 10.16016/j.1000-5404.201910187.

Shuang YOU, Shuaibin LIU, Xiaolan OUYANG, et al. Therapeutic effect of collagen on vaginal atrophy in ovariectomized rats[J]. Journal of Third Military Medical University, 2020, 42(6): 632-638，封三. DOI： 10.16016/j.1000-5404.201910187.

摘要

Abstract

Objective

To evaluate the therapeutic effect of collagen on vaginal atrophy (VA) in ovariectomized rats.

Methods

Forty female SD rats were randomized into sham-operated group

VA model group

collagen group and estrogen group (

=10). In the latter 3 groups

rat models of VA were established by ovariectomy and treated with collagen or estrogen for 14 consecutive days starting from the 4th week after the operation. Body weight and vaginal pH ualue of the rats were measured daily

and the vaginal tissues were sampled after the treatments to examine the expression levels of PCNA

MMP-1

TIMP-1

COL-Ⅰ

COL-Ⅲ and TGF-β1 using immunohistochemistry; the mRNA expression levels of MMP-1

TIMP-1

COL-Ⅰ

COL-Ⅲ

and TGF-β1 were detected using real-time PCR. Western blotting was used to detect the protein levels of TGF-β1

Smad2/3 and p-Smad2/3 in the vaginal tissues.

Results

The vaginal pH ualue of the ovariectomized rats decreased obviously after collagen treatment

which significantly increased the thickness and proliferative activity of the vaginal epithelium

enhanced the synthesis and lowered the degradation of extracellular matrix components

and obviously increased the expression of phosphorylated Smad2/3 protein in the vaginal tissues. Compared with estrogen treatment

collagen showed less adverse effects on the uterus.

Conclusion

Collagen can effectively alleviate vaginal atrophy

increase extracellular matrix components in the vaginal tissue and promote the repair of vaginal epithelium in ovariectomized rats.

关键词

Keywords

references

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