复方缓溃乐混悬剂对大鼠溃疡性结肠炎的治疗作用及机制

阿依加玛力·沙依提; 卡思木江·阿西木江; 许晨波; 希林古丽·吾守尔; 吴桂霞; 纳菲沙·卡德尔; 库热西·玉努斯

doi:10.16016/j.1000-5404.201910144

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复方缓溃乐混悬剂对大鼠溃疡性结肠炎的治疗作用及机制

基础医学 | 更新时间：2026-01-09

- 复方缓溃乐混悬剂对大鼠溃疡性结肠炎的治疗作用及机制
- Effect of Compound Huankuile Suspension on ulcerative colitis in rats and the therapeutic mechanism
- 第三军医大学学报 2020年42卷第6期页码：616-623
- 作者机构：
- 作者简介：
- 基金信息：
  
  新疆维吾尔自治区“十三五”重点学科建设项目(1001)
- DOI：10.16016/j.1000-5404.201910144
  中图分类号：
- 收稿：2019-10-20，
  
  纸质出版：2020-03-30
- 稿件说明：
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阿依加玛力·沙依提, 卡思木江·阿西木江, 许晨波, 等. 复方缓溃乐混悬剂对大鼠溃疡性结肠炎的治疗作用及机制[J]. 第三军医大学学报, 2020,42(6):616-623.

Ayijiamali SHAYITI, Kasimujiang AXIMUJIANG, Chenbo XU, et al. Effect of Compound Huankuile Suspension on ulcerative colitis in rats and the therapeutic mechanism[J]. Journal of Third Military Medical University, 2020, 42(6): 616-623.
阿依加玛力·沙依提, 卡思木江·阿西木江, 许晨波, 等. 复方缓溃乐混悬剂对大鼠溃疡性结肠炎的治疗作用及机制[J]. 第三军医大学学报, 2020,42(6):616-623. DOI： 10.16016/j.1000-5404.201910144.

Ayijiamali SHAYITI, Kasimujiang AXIMUJIANG, Chenbo XU, et al. Effect of Compound Huankuile Suspension on ulcerative colitis in rats and the therapeutic mechanism[J]. Journal of Third Military Medical University, 2020, 42(6): 616-623. DOI： 10.16016/j.1000-5404.201910144.

摘要

Abstract

Objective

To investigate the effect of Huankuile (HKL) suspension

a compound traditional Chinese herbal medicinal preparation

for treating ulcerative colitis (UC) in rats and explore its therapeutic mechanism.

Methods

Fifty male Wistar rats were randomized into normal control group

UC model group

HKL suspension intervention group

and 5-amino acid (5-ASA) intervention group. Except for those in the normal control group

all the rats were subjected to colonic enema with 2

6-trinitrobenzene sulfonic acid (TNBS) and ethanol to induce UC

followed by drug treatment for 14 d. The colonic injury index (CMDI) and pathological scores of the colon tissues were assessed

and the histopathological changes in the colon was observed with HE staining. The mRNA and protein expression levels of interleukin-6 (IL-6)

IL-17

IL-10

and transforming growth factor-β1 (TGF-β1) in the colon tissues were detected using real-time PCR and ELISA.

Results

Compared with those in the normal control group

the rats in the UC model group had markedly higher CMDI and pathological scores in the colon tissues (

0.05)

which were significantly reduced by intervention with HKL and 5-ASA (

0.05). The rats with US showed significantly increased expression of IL-6

IL-17

and IL-10 at both mRNA and protein levels in the colon tissue (

0.05) without significant changes in TGF-β1 mRNA expression but with significantly de

creased TGF-β1 protein expression (

0.05). Treatment with HKL and 5-ASA obviously lowered the mRNA and protein level of IL-6 and IL-17 and the protein expression of IL-10 (

0.05) and increased the expression level of TGF-β1 mRNA (

0.05) in the colon tissue of the rats with UC; but only treatment with HKL resulted in significant changes in the expression levels of IL-10 mRNA and TGF-β1 protein in the rats with UC (

0.05). HKL and 5-ASA showed no significant differences in their therapeutic effects on UC or in their effects on the expressions of the inflammatory factors (

0.05).

Conclusion

HKL can effectively improve the symptoms and colonic injury in rats with TNBS-induced UC possibly by down-regulating the pro-inflammatory factors IL-6 and IL-17 and up-regulating the anti-inflammatory factor TGF-β1.

关键词

Keywords

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