胎盘间充质干细胞移植对大鼠重症急性胰腺炎的保护作用

黄启林; 杨屹; 罗晨; 李帅; 汤礼军; 孙红玉

doi:10.16016/j.1000-5404.201910113

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胎盘间充质干细胞移植对大鼠重症急性胰腺炎的保护作用

基础医学 | 更新时间：2026-01-09

- 胎盘间充质干细胞移植对大鼠重症急性胰腺炎的保护作用
- Protective effect of placenta-derived mesenchymal stem cell transplantation against severe acute pancreatitis in rats
- 第三军医大学学报 2020年42卷第7期页码：646-655
- 作者机构：
  
  1.610031 成都，西南交通大学医学院
  2.610083 成都，西部战区总医院胰腺损伤与修复四川省重点实验室
- 作者简介：
  
  [孙红玉，E-mail: shongyu2008@163.com SUN Hongyu, E-mail: shongyu2008@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81772001);国家临床重点专科建设项目(41792113)
- DOI：10.16016/j.1000-5404.201910113
  中图分类号： R329.1;R576;R617
- 收稿：2019-10-18，
  
  修回：2019-12-13，
  
  纸质出版：2020-04-15
- 稿件说明：
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黄启林, 杨屹, 罗晨, 等. 胎盘间充质干细胞移植对大鼠重症急性胰腺炎的保护作用[J]. 第三军医大学学报, 2020,42(7):646-655.

Qilin HUANG, Yi YANG, Chen LUO, et al. Protective effect of placenta-derived mesenchymal stem cell transplantation against severe acute pancreatitis in rats[J]. Journal of Third Military Medical University, 2020, 42(7): 646-655.
黄启林, 杨屹, 罗晨, 等. 胎盘间充质干细胞移植对大鼠重症急性胰腺炎的保护作用[J]. 第三军医大学学报, 2020,42(7):646-655. DOI： 10.16016/j.1000-5404.201910113.

Qilin HUANG, Yi YANG, Chen LUO, et al. Protective effect of placenta-derived mesenchymal stem cell transplantation against severe acute pancreatitis in rats[J]. Journal of Third Military Medical University, 2020, 42(7): 646-655. DOI： 10.16016/j.1000-5404.201910113.

摘要

目的

研究胎盘间充质干细胞(placenta-derived mesenchymal stem cells

P-MSCs)对大鼠重症急性胰腺炎(severe acute pancreatitis

SAP)的保护作用。

方法

健康成年雄性SD大鼠32只，随机分为对照组、P-MSCs组、SAP组和SAP+P-MSCs组，每组8只。采用4%牛磺胆酸钠胆胰管逆行注射法建立SAP大鼠模型，造模结束后12 h与3

6 h经尾静脉注射P-MSCs(1×10

个/100 g)。大鼠在P-MSCs第一次输注后72 h处死，HE染色评估胰腺损伤程度，ELISA法测定血清中炎症因子、淀粉酶与脂肪酶浓度，qRT-PCR检测胰腺组织中炎症因子基因表达水平，免疫组化法检测胰腺组织中巨噬细胞浸润情况。

结果

体外输注的P-MSCs有向损伤部位定植的特性。与SAP组相比，SAP+P-MSCs组胰腺病理评分明显降低(

0.05)；血清中淀粉酶、脂肪酶、IL-1β、IL-6与TNF-α浓度明显降低(

0.05)，而IL-10、IL-4和TGF-β浓度明显升高(

0.05)；胰腺组织中IL-1β与TNF-α mRNA表达水平明显降低(

0.05)，IL-10与IL-4 mRNA表达水平明显升高(

0.05)。此外，P-MSCs可以有效减轻胰腺组织中巨噬细胞浸润。

结论

P-MSCs能有效减轻SAP大鼠胰腺局部炎症反应与全身炎症反应，其减轻胰腺局部炎症反应可能与其减少胰腺局部巨噬细胞与中性粒细胞浸润有关。

Abstract

Objective

To investigate the protective effects of human placenta-derived mesenchymal stem cell (P-MSC) transplantation against severe acute pancreatitis (SAP) in rats.

Methods

A total of 32 healthy adult male Sprague-Dawley (SD) rats were randomly divided into control group

P-MSC group

SAP and SAP+P-MSCs group (

=8)

and in the latter 2 groups

SAP was induced by retrograde injection of 4% sodium taurocholate into the common biliopancreatic duct. P-MSCs (1×10

cells/100 g) were delivered through the tail vein of the rats at 12 and 36 h after the induction of SAP. All the rats were sacrificed 72 h after the first P-MSC infusion. HE staining was used to assess the pancreatic pathologies of the rats after the treatments. The serum levels of the pro- and anti-inflammatory mediators

amylase and lipase were determined by ELISA

and the mRNA expression levels of the inflammatory mediators in the pancreatic tissue were detected using qRT-PCR. Immunohistochemistry was performed to examine macrophage infiltration in the pancreatic tissue of the rats.

Results

Homing of the infused P-MSCs to the injury site was observed in the rats with SAP. Transplantation of P-MSCs significantly ameliorated histopatholo

gical injury and lowered pancreatic pathological score of the pancreas

reduced serum levels of amylase

lipase

interleukin-1β (IL-1β)

IL-6 and tumor necrosis factor-α (TNF-α) (

0.05)

increased serum levels of IL-10

IL-4 and transforming growth factor-β (TGF-β) (

0.05)

decreased the expression levels of IL-1β and TNF-α (

0.05) and increased the expression of IL-10 and IL-4 in the pancreatic tissue (

0.05) of the rats with SAP. In addition

P-MSC transplantation effectively reduced macrophage infiltration in the pancreatic tissue of the rats.

Conclusion

Transplantation of P-MSCs can effectively alleviate local inflammatory response in the pancreas and systemic inflammatory response in rats with SAP probably by reducing local macrophage and neutrophil infiltration in the pancreas.

关键词

Keywords

references

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