Toll样受体4抑制剂对新生大鼠缺氧缺血后脑海马神经元及树突棘的影响

朱杏; 唐振; 向希盈; 孙艳燕; 王博; 黑明燕

doi:10.16016/j.1000-5404.201910089

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Toll样受体4抑制剂对新生大鼠缺氧缺血后脑海马神经元及树突棘的影响

基础医学 | 更新时间：2026-01-09

- Toll样受体4抑制剂对新生大鼠缺氧缺血后脑海马神经元及树突棘的影响
- Effects of Toll-like receptor 4 inhibitor on hypoxic-ischemic hippocampal neurons and dendritic spines in neonatal rats
- 第三军医大学学报 2020年42卷第8期页码：815-821
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81671505);北京市自然科学基金项目(7192061)
- DOI：10.16016/j.1000-5404.201910089
  中图分类号：
- 收稿：2019-10-15，
  
  纸质出版：2020-04-30
- 稿件说明：
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朱杏, 唐振, 向希盈, 等. Toll样受体4抑制剂对新生大鼠缺氧缺血后脑海马神经元及树突棘的影响[J]. 第三军医大学学报, 2020,42(8):815-821.

Xing ZHU, Zhen TANG, Xiying XIANG, et al. Effects of Toll-like receptor 4 inhibitor on hypoxic-ischemic hippocampal neurons and dendritic spines in neonatal rats[J]. Journal of Third Military Medical University, 2020, 42(8): 815-821.
朱杏, 唐振, 向希盈, 等. Toll样受体4抑制剂对新生大鼠缺氧缺血后脑海马神经元及树突棘的影响[J]. 第三军医大学学报, 2020,42(8):815-821. DOI： 10.16016/j.1000-5404.201910089.

Xing ZHU, Zhen TANG, Xiying XIANG, et al. Effects of Toll-like receptor 4 inhibitor on hypoxic-ischemic hippocampal neurons and dendritic spines in neonatal rats[J]. Journal of Third Military Medical University, 2020, 42(8): 815-821. DOI： 10.16016/j.1000-5404.201910089.

摘要

Abstract

Objective

To evaluate the effects of preconditioning with Toll-like receptor 4 (TLR4) inhibitor on hypoxic-ischemic hippocampal neurons and dendritic spines in neonatal rats.

Methods

Seventy-five neonatal (7-day-old) rats were randomly divided into control group

hypoxia-ischemia group (HI group) and TAK-242 (a TLR4-specific antagonist) group. In the latter 2 groups

rat models of hypoxic-ischemic brain damage (HIBD) were established using the Rice method

and in TAK-242 group

a single dose of TAK-242 (0.5 mg/kg) was intraperitoneally injected 30 min prior to HI. Western blotting was used to detect TLR4 expression in the left hippocampus of the rats 24 h after HI. On day 21 following the modeling

HE staining

Nissl staining and Golgi silver staining were used to assess neuronal loss and the changes in dendritic spine density in the left hippocampus. The expression of N-methyl-D-aspartic acid receptors 2A and 2B (NR2A and NR2B) was detected using Western blotting and immunofluorescence staining.

Results

The expression of TLR4 in the left hippocampus of the neonatal rats increased significantly at 24 h after HI (

0.05)

and TAK-242 obviously suppressed the up-regulated expression of TLR4 (

0.05). Compared with those in the control group

the number of neurons

dendritic spine density and the expression of NR2A all decreased significantly in the left hippocampus of the rats at 21 d after HI (

0.05); pretreatment with TAK-242 obviously alleviated these changes (

0.05).

Conclusion

TLR4 inhibitor can mitigate HI-induced long-term damage of the hippocampal neurons and the dendritic spines.

关键词

Keywords

references

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