紫檀芪改善睡眠限制小鼠运动耐力的作用及其机制

刘洋; 郎和东; 惠锁成; 陈卡; 糜漫天

doi:10.16016/j.1000-5404.201909220

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紫檀芪改善睡眠限制小鼠运动耐力的作用及其机制

基础医学 | 更新时间：2026-01-09

- 紫檀芪改善睡眠限制小鼠运动耐力的作用及其机制
- Pterostilbene improves exercise intolerance induced by sleep restriction in mice by activating AMPK/SIRT1 signaling and promoting mitochondrial biosynthesis
- 第三军医大学学报 2020年42卷第3期页码：259-266
- 作者机构：
- 作者简介：
- 基金信息：
  
  全军后勤科研重大项目(AWS17J014)
- DOI：10.16016/j.1000-5404.201909220
  中图分类号：
- 收稿：2019-09-29，
  
  纸质出版：2020-02-15
- 稿件说明：
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刘洋, 郎和东, 惠锁成, 等. 紫檀芪改善睡眠限制小鼠运动耐力的作用及其机制[J]. 第三军医大学学报, 2020,42(3):259-266.

Yang LIU, Hedong LANG, Suocheng HUI, et al. Pterostilbene improves exercise intolerance induced by sleep restriction in mice by activating AMPK/SIRT1 signaling and promoting mitochondrial biosynthesis[J]. Journal of Third Military Medical University, 2020, 42(3): 259-266.
刘洋, 郎和东, 惠锁成, 等. 紫檀芪改善睡眠限制小鼠运动耐力的作用及其机制[J]. 第三军医大学学报, 2020,42(3):259-266. DOI： 10.16016/j.1000-5404.201909220.

Yang LIU, Hedong LANG, Suocheng HUI, et al. Pterostilbene improves exercise intolerance induced by sleep restriction in mice by activating AMPK/SIRT1 signaling and promoting mitochondrial biosynthesis[J]. Journal of Third Military Medical University, 2020, 42(3): 259-266. DOI： 10.16016/j.1000-5404.201909220.

摘要

Abstract

Objective

To investigate the effect of pterostilbene (PTE) on exercise intolerance induced by sleep restriction (SR) in mice and explore the underlying mechanism.

Methods

Thirty male C57BL/6J mice were randomized into control group

SR group and SR with PTE treatment (SR+PTE) group. The mice in the latter 2 groups were kept awake using a sleep deprivation apparatus with their daily sleep time limited to 4 h for 5 consecutive days. In SR+PTE group

the mice received PTE treatment by gavage at a daily dose of 100 mg/kg (administered at 08:00 am). After the 5-day treatment

body weight changes of the mice were recorded

and a weight-loaded forced swimming test was performed; blood samples were collected for detecting serum levels of biochemical indicators of exercise fatigue. HE staining was used to observe the pathological changes of the skeletal muscle tissue. The mitochondrial DNA (mtDNA) content and the expressions of AMPK

SIRT1 and mitochondrial biogenesis-related genes were determined by real-time PCR and Western blotting.

Results

Compared with those in the control group

the mice in both SR and SR+PTE groups showed significant weight loss after the treatment (

0.05)

and the decrease was more obvious in SR group (

0.05). Compared with those in SR group

the mice in SR+PTE group had significantly prolonged exhaustive swimming time and significantly lowered serum levels of LDH and CK (

0.05). No significant differences were found in skeletal muscle pathologies among the groups. The mtDNA content and expression levels of mitochondrial biosynthesis-related genes including PGC1α

NRF1

ERRα and TFAM were significantly higher in SR+PTE group than in SR group (

0.05). Western blotting showed that the expression levels of p-AMPK and SIRT1 were significantly higher in SR+PTE group than in SR group (

0.05)

while that of AMPK were comparable between the 2 groups.

Conclusion

PTE can improve exercise intolerance in mice exposed to SR possibly by activating AMPK/SIRT1 signaling pathway to enhance mitochondrial biogenesis.

关键词

Keywords

references

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