DUSP1在缺氧RAW264.7细胞中的表达及其对炎症免疫反应的调控作用

董华平; 刘宝; 张二龙; 高钰琪

doi:10.16016/j.1000-5404.201909198

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DUSP1在缺氧RAW264.7细胞中的表达及其对炎症免疫反应的调控作用

军事医学：特殊环境军事医学 | 更新时间：2026-01-09

- DUSP1在缺氧RAW264.7细胞中的表达及其对炎症免疫反应的调控作用
- Expression of dual specificity phosphatase-1 in hypoxic RAW264.7 cells and its role in inflammatory immune response regulation
- 第三军医大学学报 2020年42卷第6期页码：560-567
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点项目(81830062)
- DOI：10.16016/j.1000-5404.201909198
  中图分类号：
- 收稿：2019-09-20，
  
  纸质出版：2020-03-30
- 稿件说明：
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董华平, 刘宝, 张二龙, 等. DUSP1在缺氧RAW264.7细胞中的表达及其对炎症免疫反应的调控作用[J]. 第三军医大学学报, 2020,42(6):560-567.

Huaping DONG, Bao LIU, Erlong ZHANG, et al. Expression of dual specificity phosphatase-1 in hypoxic RAW264.7 cells and its role in inflammatory immune response regulation[J]. Journal of Third Military Medical University, 2020, 42(6): 560-567.
董华平, 刘宝, 张二龙, 等. DUSP1在缺氧RAW264.7细胞中的表达及其对炎症免疫反应的调控作用[J]. 第三军医大学学报, 2020,42(6):560-567. DOI： 10.16016/j.1000-5404.201909198.

Huaping DONG, Bao LIU, Erlong ZHANG, et al. Expression of dual specificity phosphatase-1 in hypoxic RAW264.7 cells and its role in inflammatory immune response regulation[J]. Journal of Third Military Medical University, 2020, 42(6): 560-567. DOI： 10.16016/j.1000-5404.201909198.

摘要

Abstract

Objective

To investigate the expression of dual specificity phosphatase-1 (DUSP1) in hypoxic RAW264.7 macrophages and its regulatory role in hypoxia-induced RAW264.7 macrophage inflammatory immune response.

Methods

RAW264.7 macrophages were cultured under a hypoxic condition (1%O

) for 12

24 and 36 h

with the cells maintained in routine culture conditions (21%O

) as control. RNA interference technique was used to inhibit Dusp1 expression in RAW264.7 cells

which were exposed to hypoxia for 24 h following the transfection. The cells and the culture supernatant were collected after the treatments

and the mRNA expression levels of

Dusp

10 and

Tnfa

were detected using qRT-PCR; and the protein expression of DUSP1 was detected using Western blotting

and the levels of interleukin-1β (IL-1β)

IL-10 and tumor necrosis factor-α (TNF-α) in the supernatant were determined with ELISA.

Results

Compared with the control cells cultured in normoxic condition

RAW264.7 cells with hypoxic exposures exhibited significantly increased the mRNA and protein levels of DUSP1 (

0.05)

whose protein expression reached the peak level at 24 h of hypoxia. The mRNA levels of

and

10increased significantly after hypoxic exposure for 24 and 36 h (

0.05)

and the expression of Tnfa mRNA reduced significantly after a 12-h exposure (

0.05). Hypoxic exposure caused a significant increase in the secretion level of IL-1β by the macrophages (

0.05). Transfection with si-

Dusp

1 significantly increased the mRNA expression of

and the secretion levels of IL-1β and IL-10 and significantly lowered the mRNA expression and secretion level of TNF-α in hypoxic RAW264.7 cells (all

0.05).

Conclusion

Hypoxia can up-regulate DUSP1 expression in RAW264.7 macrophages. DUSP1 participates in the regulation of hypoxia-induced RAW264.7 macrophage inflammatory immune response by modulating the expression and the secretion of IL-1β

IL-10 and TNF-α.

关键词

Keywords

references

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