低氧对小鼠生精小管的损伤作用及对精母细胞凋亡的诱导作用

殷骏; 柳君泽; 倪兵; 唐中伟; 高志奇; 赵力; 张梦洁; 陈德伟; 何文娟; 廖卫公

doi:10.16016/j.1000-5404.201909114

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低氧对小鼠生精小管的损伤作用及对精母细胞凋亡的诱导作用

军事医学：特殊环境军事医学 | 更新时间：2026-01-09

- 低氧对小鼠生精小管的损伤作用及对精母细胞凋亡的诱导作用
- Hypoxia causes seminiferous tubule damage by inducing apoptosis of spermatocytes in mice
- 第三军医大学学报 2020年42卷第6期页码：576-582
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81701855)
- DOI：10.16016/j.1000-5404.201909114
  中图分类号：
- 收稿：2019-09-16，
  
  纸质出版：2020-03-30
- 稿件说明：
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殷骏, 柳君泽, 倪兵, 等. 低氧对小鼠生精小管的损伤作用及对精母细胞凋亡的诱导作用[J]. 第三军医大学学报, 2020,42(6):576-582.

Jun YIN, Junze LIU, Bing NI, et al. Hypoxia causes seminiferous tubule damage by inducing apoptosis of spermatocytes in mice[J]. Journal of Third Military Medical University, 2020, 42(6): 576-582.
殷骏, 柳君泽, 倪兵, 等. 低氧对小鼠生精小管的损伤作用及对精母细胞凋亡的诱导作用[J]. 第三军医大学学报, 2020,42(6):576-582. DOI： 10.16016/j.1000-5404.201909114.

Jun YIN, Junze LIU, Bing NI, et al. Hypoxia causes seminiferous tubule damage by inducing apoptosis of spermatocytes in mice[J]. Journal of Third Military Medical University, 2020, 42(6): 576-582. DOI： 10.16016/j.1000-5404.201909114.

摘要

Abstract

Objective

To investigate the damage of seminiferous tubules in mice exposed to hypoxia and the effects of hypoxia on apoptosis of mouse spermatocytes in the seminiferous tubules.

Methods

Forty 3-week-old male Balb/c mice were randomly assigned into a normoxia group (housed in a normoxic condition at an altitude of 300 m) and 3 hypoxia groups exposed to hypoxia for 15

30 or 60 d in a hypobaric chamber simulating the condition at an altitude of 6 000 m. After hypoxic exposures

the mice were euthanized and the testicular tissues were examined for pathological changes in the seminiferous tubules using HE and TUNEL staining. A mouse spermatocyte-derived cell line

GC-2spd

was also cultured in a normoxic condition or a hypoxic condition (1% O

and 5% CO

) for 48

or 72 h

and the cell apoptosis was detected using flow cytometry and TUNEL staining and by examining LDH

caspase-3

caspase-8

and caspase-9 levels in the culture medium.

Results

The apoptotic rates of the s

permatocytes in the testicular seminiferous tubules of the mice increased significantly after hypoxic exposure for 15

30 and 60 d as compared with the apoptosis rate of (4.6±1.4)% in the normoxia group (

0.01). In cultured GC-2spd cells

hypoxic exposure for 48

60 and 72 h also significantly increased the cell apoptotic rate to (15.43±1.47)%

(44.10±14.10)% and (63.36±12.74)%

as compared with the rates of (4.18±1.40)%

(5.45%±2.30)%

and (14.47±8.21)% in the normoxia group

respectively (

0.01). Microscopically

the numbers of apoptotic spermatocytes per field were 22±3

55±5

and 82±12 in the hypoxia group at 48

60 and 72 h

respectively

significantly greater than those in the normoxia group (5±1

8±2

and 15±4

respectively;

0.01). The absolute value of LDH activity in cultured GC-2spd cells at 48 h was significantly higher in the hypoxia group than in the normoxia group (1.28±0.18

0.48±0.09

0.01); the values of caspase-3/8/9 in the hypoxia group at 48 h were 12.20±0.40

22.73±0.60

and 10.90±0.40

respectively

significantly higher than those in the normoxia group (7.67±0.45

16.20±0.56

and 7.03±0.25

respectively

0.01).

Conclusion

Exposure to hypoxia simulating the condition at an altitude of 6 000 m for 15 d can cause decreased spermatogenesis and increased apoptosis of the spermatogenic cells in mice

suggesting that spermatocyte apoptosis is one of the mechanisms by which hypobaric hypoxia reduces spermatogenesis.

关键词

Keywords

references

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