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橘络黄酮对急性缺氧肠平滑肌细胞的保护作用
Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism
- 第三军医大学学报 2020年42卷第6期 页码：594-600
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(31971106);国家自然科学基金面上项目(81372124);BWS(18CXZ044);BWS(16J010)
DOI：10.16016/j.1000-5404.201908220
中图分类号：收稿：2019-08-31，
纸质出版：2020-03-30
- 稿件说明：
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刘景君, 王晓明, 沈成凤, 等. 橘络黄酮对急性缺氧肠平滑肌细胞的保护作用[J]. 第三军医大学学报, 2020,42(6):594-600.
Jingjun LIU, Xiaoming WANG, Chengfeng SHEN, et al. Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism[J]. Journal of Third Military Medical University, 2020, 42(6): 594-600.
刘景君, 王晓明, 沈成凤, 等. 橘络黄酮对急性缺氧肠平滑肌细胞的保护作用[J]. 第三军医大学学报, 2020,42(6):594-600. DOI： 10.16016/j.1000-5404.201908220.
Jingjun LIU, Xiaoming WANG, Chengfeng SHEN, et al. Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism[J]. Journal of Third Military Medical University, 2020, 42(6): 594-600. DOI： 10.16016/j.1000-5404.201908220.
摘要
Abstract
Objective
2
To investigate the protective effect of citrus flavone on cultured human intestinal smooth muscle cells (HISMCs) against acute hypoxia and explore the mitochondrial mechanism.
Methods
2
Cultured HISMCs were exposed to acute hypoxia with 1%
3%
or 5% O
2
(5% CO
2
94% N
2
) for 6 h or with 1% O
2
for 3
6
and 9 h. The cells cultured in 1% O
2
(5% CO
2
94% N
2
) were treated with citrus flavonoids (300 or 600 μg/mL) for 6 h. Mitochondrial membrane potential and the cell apoptotic rate were determined with flow cytometry
and the activities of caspase-3 and caspase-8 were detected using Ac-DEVD-pNA and AC-IETD-pNA colorimetry
respectively. The total content of TR3 nuclear orphan receptor
(TR3) in the hypoxic cells and its content in the mitochondria were determined with Western blotting. We also tested the effect of TR3 overexpression on the protective effect of 600 μg/mL citrus flavonoids in the HISMCs exposed to 1% O
2
.
Results
2
Acute hypoxia resulted in significantly decreased mitochondrial membrane potential
enhanced caspase-3 and caspase-8 activities
and increased apoptosis in cultured HISMCs (
P
<
0.05). Citrus flavonoids effectively enhanced mitochondrial membrane potential level
suppressed the mitochondrial apoptotic pathway (
P
<
0.05)
and inhibited the translocation of TR3 to the mitochondria in the hypoxic cells. Overexpression of TR3 significantly attenuated the protective effect of citrus flavonoids on the hypoxic cells (
P
<
0.05).
Conclusion
2
Citrus flavonoids protect intestinal smooth muscle cells against hypoxic injury by regulating mitochondrial translocation of TR3.
关键词
Keywords
references
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