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模拟高原间断缺氧暴露对雌性大鼠动情周期的影响
Impact of intermittent hypoxic exposure on estrous cycle in female rats
- 第三军医大学学报 2020年42卷第6期 页码：583-587
- 作者机构：
- 作者简介：
- 基金信息：全军后勤科研重点项目(BWS17J025);国家自然科学基金青年项目(31600955)
DOI：10.16016/j.1000-5404.201908216
中图分类号：收稿：2019-08-30，
纸质出版：2020-03-30
- 稿件说明：
移动端阅览
蒲玲玲, 邓炳楠, 徐洪宝, 等. 模拟高原间断缺氧暴露对雌性大鼠动情周期的影响[J]. 第三军医大学学报, 2020,42(6):583-587.
Lingling PU, Bingnan DENG, Hongbao XU, et al. Impact of intermittent hypoxic exposure on estrous cycle in female rats[J]. Journal of Third Military Medical University, 2020, 42(6): 583-587.
蒲玲玲, 邓炳楠, 徐洪宝, 等. 模拟高原间断缺氧暴露对雌性大鼠动情周期的影响[J]. 第三军医大学学报, 2020,42(6):583-587. DOI： 10.16016/j.1000-5404.201908216.
Lingling PU, Bingnan DENG, Hongbao XU, et al. Impact of intermittent hypoxic exposure on estrous cycle in female rats[J]. Journal of Third Military Medical University, 2020, 42(6): 583-587. DOI： 10.16016/j.1000-5404.201908216.
摘要
Abstract
Objective
2
To investigate the effect of intermittent hypoxic exposure on estrous cycle in female rats.
Methods
2
Healthy female SD rats were randomized after 5 d of adaptive feeding into normal control group maintained in routine conditions and hypoxic exposure group with daily hypoxic exposure simulating the condition at an altitude of 6 000 m for 8 h in a hypobaric chamber (
n
=11). During the 4 weeks of exposure
the rats were allowed free access to standard laboratory chow and sterile water. The food intake of the rats was recorded daily and their body weight twice a week. The organ /body weight ratios were used to assess the effects of hypoxic exposure on the vital organ coefficients. The morphological changes of vaginal exfoliated cells were examined twice a day to assess the changes of the estrous cycle. After the exposure
aortic blood samples were collected from all the rats to determine the serum levels of sex hormones and malondialdehyde(MDA) and the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px).
Results
2
Compared with the normal control group
the rats within 7 d of hypoxic exposure showed significantly decreased food intake
and significant decrease in body weight occurred within 10 d of the exposure. Hypoxic exposure significantly increased the organ coefficients of the kidney
liver and spleen but did not significantly affect the uterus and ovaries of the female rats. Morphological analysis of the exfoliated cells showed that hypoxic exposure significantly prolonged the estrus and metestrus phase and shortened the diestrus period of the rats. Hypoxic exposure resulted in significantly decreased serum levels of progesterone and luteinizing hormone
lowered the activities of GSH-Px and SOD and increased MDA content as compared with those in the control group.
Conclusion
2
Hypoxic exposure can cause estrous cycle disorder in female rats possibly by inducing oxidative stress.
关键词
Keywords
references
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