莱菔硫烷对肝细胞的抗氧化能力和线粒体功能的影响

雷鹏; 田思聪; 滕春莹; 孙瑶; 单毓娟; 李宝龙

doi:10.16016/j.1000-5404.201908073

您当前的位置：

首页 >

文章列表页 >

莱菔硫烷对肝细胞的抗氧化能力和线粒体功能的影响

基础医学 | 更新时间：2026-01-09

- 莱菔硫烷对肝细胞的抗氧化能力和线粒体功能的影响
- Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress
- 第三军医大学学报 2020年42卷第2期页码：141-147
- 作者机构：
  
  1.150001 哈尔滨, 哈尔滨工业大学化工与化学学院食品科学与工程系
  2.150040 哈尔滨, 黑龙江省中医药大学药物安全评价中心
- 作者简介：
  
  [单毓娟，E-mail: yujuan72@163.com SHAN Yujuan, E-mail: yujuan72@163.com
  [李宝龙，E-mail: lbl73@163.com LI Baolong, E-mail: lbl73@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81573135)
- DOI：10.16016/j.1000-5404.201908073
  中图分类号： R282.71;R322.47;R329.25
- 收稿：2019-08-12，
  
  修回：2019-11-05，
  
  纸质出版：2020-01-30
- 稿件说明：
移动端阅览
雷鹏, 田思聪, 滕春莹, 等. 莱菔硫烷对肝细胞的抗氧化能力和线粒体功能的影响[J]. 第三军医大学学报, 2020,42(2):141-147.

Peng LEI, Sicong TIAN, Chunying TENG, et al. Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress[J]. Journal of Third Military Medical University, 2020, 42(2): 141-147.
雷鹏, 田思聪, 滕春莹, 等. 莱菔硫烷对肝细胞的抗氧化能力和线粒体功能的影响[J]. 第三军医大学学报, 2020,42(2):141-147. DOI： 10.16016/j.1000-5404.201908073.

Peng LEI, Sicong TIAN, Chunying TENG, et al. Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress[J]. Journal of Third Military Medical University, 2020, 42(2): 141-147. DOI： 10.16016/j.1000-5404.201908073.

摘要

目的

探讨莱菔硫烷(sulforaphane，SFN)对肝细胞氧化应激和线粒体损伤的改善作用。

方法

建立高脂膳食诱导大鼠肝脏氧化应激体内模型和游离脂肪酸诱导HHL-5肝细胞氧化应激的体外模型。ELISA检测肝组织中丙二醛(malondialdehyde

MDA)的含量，Western blot测定大鼠肝脏组织中抗氧化酶血红素加氧酶-1(hemeoxygenase-1

HO-1)，磷酸酰胺腺嘌呤二核苷酸醌氧化还原酶-1(NADH dehydrogenase quinone 1

NQO1)和谷胱甘肽-S-转移酶(glutathione S-transferase

GST)的蛋白表达。采用荧光显微镜观察HHL-5肝细胞的活性氧(reactive oxygen species

ROS)含量和线粒体膜电位；ELISA测定HHL-5肝细胞中谷胱甘肽(glutathione

GSH)和超氧化物歧化酶(superoxide dismutase

SOD)的水平。实时定量PCR法检测mtDNA含量。

结果

SFN减少由高脂饲料引起MDA含量的增多(

0.05)，并上调大鼠肝组织中的抗氧化酶HO-1(

0.05)，NQO1(

0.05)和GST(

0.05)的蛋白表达。SFN降低由FFA提高HHL-5细胞的ROS水平(

0.05)，并提高其GSH(

0.05)和SOD(

0.05)的水平，以及线粒体膜电位(

0.05)。此外，SFN显著提高HHL-5细胞的mtDNA含量(

0.05)。

结论

SFN通过增加肝细胞抗氧化能力改善高脂诱导的肝脏氧化应激损伤并对肝脏线粒体功能有保护作用。

Abstract

Objective

To investigate the effect of sulforaphane (SFN) on oxidative stress and mitochondrial injury in hepatocytes.

Methods

We examined malondialdehyde (MDA) content by ELISA in the liver tissues and the protein expression of the antioxidant enzymes including hemeoxygenase-1 (HO-1)

NADH dehydrogenase quinone 1 (NQO1) and glutathione S-transferase (GST) using Western blotting in the liver tissues from a rat model of hepatic oxidative stress established by high-fat diet. We also assessed the level of reactive oxygen species (ROS) and mitochondrial membrane protential by fluorescence microscopy

tested glutathione (GSH) and superoxide dismutase (SOD) levels using ELISA

and detected mitochondrial DNA (mtDNA) content using real-time quantitative PCR in cultured hepatocytes (HHL-5 cells) under fatty acid-induced oxidative stress.

Results

In the rat model of hepatic oxidative stress

SFN significantly reduced high-fat diet-induced elevation of MDA l

evel (

0.05) and up-regulated the protein expression of the antioxidant enzymes in the liver tissues (

0.05). In cultured HHL-5 cells

SFN obviously reduced free fatty acid-induced elevation of ROS level (

0.05) and increased GSH content (

0.05)

SOD level (

0.05)

mitochondrial membrane potential (

0.05)

and the content of mtDNA (

0.05).

Conclusion

SFN can enhance the antioxidant capacity of hepatocytes to improve high-fat diet-induced hepatic oxidative stress in rats and provide protection of mitochondrial function in the hepatocytes.

关键词

Keywords

references

NOEMAN S A, HAMOODA H E, BAALASH A A. Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats[J]. Diabetol Metab Syndr, 2011, 3(1): 17. DOI: 10.1186/1758-5996-3-17.

HE H J, WANG G Y, GAO Y, et al . Curcumin attenuates Nrf2 signaling defect, oxidative stress in muscle and glucose intolerance in high fat diet-fed mice[J ] . World J Diabetes, 2012, 3(5): 94-104. DOI: 10.4239/wjd.v3.i5.94.

CARMIEL-HAGGAI M, CEDERBAUM A I, NIETO N. A high-fat diet leads to the progression of non-alcoholic fatty liver disease in obese rats[J]. FASEB J, 2005, 19(1): 136-138. DOI: 10.1096/fj.04-2291fje.

YUZEFOVYCH L V, MUS IYENKO S I, WILSON G L, et al . Mitochondrial DNA damage and dysfunction, and oxidative stress are associated with endoplasmic reticulum stress, protein degradation and apoptosis in high fat diet-induced insulin resistance mice[J ] . PLoS ONE, 2013, 8(1): e54059. DOI: 10.1371/journal.pone.0054059.

CHOI J W, OHN J H, JUNG H S, et al . Carnitine induces autophagy and restores high-fat diet-induced mitochondrial dysfunction[J ] . Metab Clin Exp, 2018, 78: 43-51. DOI: 10.1016/j.metabol.2017.09.005.

CHEN Y J, MYRACLE A D, WALLIG M A, et al . Dietary broccoli protects against fatty liver development but not against progression of liver cancer in mice pretreated with diethylnitrosamine[J ] . J Funct Foods, 2016, 24: 57-62. DOI: 10.1016/j.jff.2016.03.028.

YANG G, LEE H E, LEE J Y. A pharmacological inhibitor of NLRP3 inflammasome prevents non-alcoholic fatty liver disease in a mouse model induced by high fat diet[J]. Sci Rep, 2016, 6: 24399. DOI: 10.1038/srep24399.

ZHANG H Q, CHEN S Y, WANG A S, et al . Sulforaphane induces adipocyte Browning and promotes glucose and lipid utilization[J ] . Mol Nutr Food Res, 2016, 60(10): 2185-2197. DOI: 10.1002/mnfr.201500915.

CHEN Y J, WALLIG M A, JEFFERY E H. Dietary broccoli lessens development of fatty liver and liver cancer in mice given diethylnitrosamine and fed a Western or control diet[J]. J Nutr, 2016, 146(3): 542-550. DOI: 10.3945/jn.115.228148.

HAYES J D, DINKOVA-KOSTOVA A T. The Nrf2 regulatory network provides an interface between redox and intermediary metabolism[J]. Trends Biochem Sci, 2014, 39(4): 199-218. DOI: 10.1016/j.tibs.2014.02.002.

ANHÊ F F, NACHBAR R T, VARIN T V, et al . A polyphenol-rich cranberry extract reverses insulin resistance and hepatic steatosis independently of body weight loss[J ] . Mol Metab, 2017, 6(12): 1563-1573. DOI: 10.1016/j.molmet.2017.10.003.

李丹, 暴永平, 吴坤.莱菔硫烷诱导人肝细胞硫氧还蛋白还原酶表达[J].中国公共卫生, 2009, 25(2): 189-191. DOI: 10.3321/j.issn: 1001-0580.2009.02.032.

LI D, BAO Y P, WU K. Induction of TR1 by sulforaphane in human immortalized hepatocytes[J]. Chin J Public Health, 2009, 25(2): 189-191. DOI: 10.3321/j.issn: 1001-0580.2009.02.032.

NGUYEN D, SAMSON S L, REDDY V T, et al . Impaired mitochondrial fatty acid oxidation and insulin resistance in aging: novel protective role of glutathione[J ] . Aging Cell, 2013, 12(3): 415-425. DOI: 10.1111/acel.12073.

PIAO C S, GAO S, LEE G H, et al . Sulforaphane protects ischemic injury of hearts through antioxidant pathway and mitochondrial K(ATP) channels[J ] . Pharmacol Res, 2010, 61(4): 342-348. DOI: 10.1016/j.phrs.2009.11.009.

GRECO T, SHAFER J, FISKUM G. Sulforaphane inhibits mitochondrial permeability transition and oxidative stress[J]. Free Radic Biol Med, 2011, 51(12): 2164-2171. DOI: 10.1016/j.freeradbiomed.2011.09.017.

PETERSEN K F, BEFROY D, DUFOUR S, et al . Mitochondrial dysfunction in the elderly: possible role in insulin resistance[J ] . Science, 2003, 300(5622): 1140-1142. DOI: 10.1126/science.1082889.

ARRUDA A P, PERS B M, PARLAKGVL G, et al . Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity[J ] . Nat Med, 2014, 20(12): 1427-1435. DOI: 10.1038/nm.3735.

PIANTADOSI C A, CARRAWAY M S, BABIKER A, et al . Heme oxygenase-1 regulates cardiac mitochondrial biogenesis via Nrf2-mediated transcriptional control of nuclear respiratory factor-1[J ] . C irc Res, 2008, 103(11): 1232-1240. DOI: 10.1161/01.RES.0000338597.71702.ad.

TIAN S C, LI B L, LEI P, et al . Sulforaphane improves abnormal lipid metabolism via both ERS-dependent XBP1/ACC & SCD1 and ERS-independent SREBP/FAS pathways[J ] . Mol Nutr Food Res, 2018, 62(6): e1700737. DOI: 10.1002/mnfr.201700737.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

mdivi-1对急性心肌缺血小鼠心脏的保护作用

莱菔硫烷下调DGAT2和ACAT1表达抑制肝脂肪变的研究

PGC-1α通过线粒体介导巨噬细胞极化状态的机制研究

莱菔硫烷抑制LPS诱导的小鼠星形胶质细胞增殖

莱菔硫烷对小鼠脊髓损伤后后肢功能的作用