不同方法检测急性移植物抗宿主病患者的血清标志物

陈婷; 李小平; 高强国; 高蕾; 刘耀; 张诚; 孔佩艳; 高力; 冯一梅; 饶军; 张曦

doi:10.16016/j.1000-5404.201907252

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不同方法检测急性移植物抗宿主病患者的血清标志物

专题报道：造血干细胞移植与细胞治疗 | 更新时间：2026-01-09

- 不同方法检测急性移植物抗宿主病患者的血清标志物
- Different methods for detecting serum markers in patients with acute graft versus host disease
- 第三军医大学学报 2019年41卷第23期页码：2280-2284
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重点研究计划重点专项(2017YFA0105502);重庆市社会事业与民生保障科技创新专项(CSTC2016shms-ztzx10003)
- DOI：10.16016/j.1000-5404.201907252
  中图分类号：
- 收稿：2019-07-31，
  
  纸质出版：2019-12-25
- 稿件说明：
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陈婷, 李小平, 高强国, 等. 不同方法检测急性移植物抗宿主病患者的血清标志物[J]. 第三军医大学学报, 2019,41(23):2280-2284.

Ting CHEN, Xiaoping LI, Qiangguo GAO, et al. Different methods for detecting serum markers in patients with acute graft versus host disease[J]. Journal of Third Military Medical University, 2019, 41(23): 2280-2284.
陈婷, 李小平, 高强国, 等. 不同方法检测急性移植物抗宿主病患者的血清标志物[J]. 第三军医大学学报, 2019,41(23):2280-2284. DOI： 10.16016/j.1000-5404.201907252.

Ting CHEN, Xiaoping LI, Qiangguo GAO, et al. Different methods for detecting serum markers in patients with acute graft versus host disease[J]. Journal of Third Military Medical University, 2019, 41(23): 2280-2284. DOI： 10.16016/j.1000-5404.201907252.

摘要

Abstract

Objective

To clarify the consistency of the results of the 2 methods for detecting serum biomarkers in patients with acute graft versus host disease (aGVHD) in order to screen out biomarkers with clinical value and to establish a biomarker screening process for aGVHD.

Methods

Sixty patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT) in our medical center from April 2016 to April 2018 were recruited in this study. Protein chip and ELISA were used to detect the serum cytokine levels in the patients with aGVHD (

=30) and those without (control group

=30).

Results

Both detection methods showed that the serum levels of IL-7 and IL-27p were significantly higher (

0.05)

while the expression level of CD127 was decreased (

0.05) in the aGVHD group than the control. Th

e expression level of IL-27 was correlated with the severity of aGVHD (

0.05). The area under the receiver operating characteristic (ROC) curve was between 0.7 and 0.9

with no significant differences between the 2 methods.

Conclusion

Both protein chip and ELISA have high accuracy and consistency in the detection of serum biomarkers in aGVHD patients. It is possible to establish a high-throughput screening of clinically significant cytokines by protein chip

and then expanding the sample size and repeating the validation by ELISA

which is conducive to clinical development.

关键词

Keywords

references

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