长链非编码RNA LINC02163在肝细胞肝癌中表达上调并促进肝癌细胞迁移和侵袭

罗毅; 房晓瑜; 谢薇佳; 周征宇; 王友豪; 王小军; 江艳; 马翔宇

doi:10.16016/j.1000-5404.201907251

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长链非编码RNA LINC02163在肝细胞肝癌中表达上调并促进肝癌细胞迁移和侵袭

临床医学 | 更新时间：2026-01-09

- 长链非编码RNA LINC02163在肝细胞肝癌中表达上调并促进肝癌细胞迁移和侵袭
- Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma
- 第三军医大学学报 2020年42卷第1期页码：81-87
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学):军事预防医学系军队流行病学教研室
  2.400038 重庆，陆军军医大学(第三军医大学):第一附属医院全军肝胆外科研究所
- 作者简介：
  
  [马翔宇，E-mail: xymacq@hotmail.com MA Xiangyu, E-mail:xymacq@hotmail.com
- 基金信息：
  
  国家自然科学基金面上项目(81302498)
- DOI：10.16016/j.1000-5404.201907251
  中图分类号： R394.3;R730.23;R735.7
- 收稿：2019-07-31，
  
  修回：2019-09-21，
  
  纸质出版：2020-01-15
- 稿件说明：
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罗毅, 房晓瑜, 谢薇佳, 等. 长链非编码RNA LINC02163在肝细胞肝癌中表达上调并促进肝癌细胞迁移和侵袭[J]. 第三军医大学学报, 2020,42(1):81-87.

Yi LUO, Xaoyu FANG, Weijia XIE, et al. Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma[J]. Journal of Third Military Medical University, 2020, 42(1): 81-87.
罗毅, 房晓瑜, 谢薇佳, 等. 长链非编码RNA LINC02163在肝细胞肝癌中表达上调并促进肝癌细胞迁移和侵袭[J]. 第三军医大学学报, 2020,42(1):81-87. DOI： 10.16016/j.1000-5404.201907251.

Yi LUO, Xaoyu FANG, Weijia XIE, et al. Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma[J]. Journal of Third Military Medical University, 2020, 42(1): 81-87. DOI： 10.16016/j.1000-5404.201907251.

摘要

目的

研究长链非编码RNA LINC02163在肝细胞肝癌(hepatocellular carcinoma，HCC)组织及肝癌细胞系中的表达及对肝癌细胞迁移、侵袭的影响。

方法

利用Quantitative Real-time PCR(qRT-PCR)检测LINC02163在5株肝癌细胞系(HuH-7、HCCLM3、MHCC-97H、SNU-182和SMMC-7721)、正常肝细胞系(L02)和60例肝癌组织及其配对癌旁组织中的差异表达情况，分析LINC02163的表达水平与肝癌患者临床病理参数的关系；利用Lipofectamine

2000转染LINC02163小干扰RNA(siRNA)至HuH-7、HCCLM3肝癌细胞内敲低LINC02163表达；通过Transwell实验检测敲低LINC02163后肝癌细胞迁移、侵袭能力的改变。

结果

LINC02163在肝癌组织中的表达水平明显高于配对癌旁组织(

0.001)；LINC02163在肝癌组织中的表达与TNM分期、微血管侵犯(MVI)明显相关(

0.01)；与正常肝细胞系(L02)比较，LINC02163在5株肝癌细胞系中高表达(

0.001)，其中在HuH-7细胞系中表达最高，其次是HCCLM3细胞系；肝癌细胞HuH-7、HCCLM3敲低LINC02163的表达后迁移和侵袭能力明显降低(

0.01)。

结论

LINC02163在肝癌组织和肝癌细胞系中的表达上调，其可促进肝癌细胞的迁移和侵袭，故可能与肝癌的发生、发展密切相关。

Abstract

Objective

To investigate the expression of long non-coding RNA LINC02163 in hepatocellular carcinoma (HCC) tissues and HCC cell lines

and its effect on the migration and invasion of hepatocellular carcinoma cells.

Methods

The differential expression level of LINC02163 in 5 HCC cell lines (HuH-7

HCCLM3

MHCC-97H

SNU-182 and SMMC-7721)

normal liver cell line (L02) and 60 HCC tissues and paired adjacent tissues was determined by quantitative real-time PCR (qRT-PCR)

and the relationship between its expression level and the clinicopathological parameters of HCC patients was analyzed. Lipofectamine

2000 was used to transfect LINC02163 small interfering RNA (siRNA) into HCC HuH-7 and HCCLM3 cells in order to knockdown the expression. Then the changes of migration and invasion ability of HCC cells after the knock-down of LINC02163 were examined by Transwell assay.

Results

The expression level of LINC02163 in HCC tissues was significantly higher than that in paired adjacent tissues (

0.001)

and the level was significantly correlated with TNM stage and microvascular invasion (MVI) in the patients (all

0.01). Compared with normal liver cell line (L02)

LINC02163 was highly expressed in 5 HCC cell lines (

0.001)

among which HuH-7 cells had the highest expression level

followed by HCCLM3 cells. LINC02163 knock-down significantly reduced the migration and invasion abilities in HuH-7 and HCCLM3 cells (

0.01).

Conclusion

LINC02163 is up-regulated in HCC tissues and cell lines and promotes the migration and invasion of HCC cells

which may be closely associated with the occurrence and development of HCC.

关键词

Keywords

references

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