地氟醚对小鼠中枢及外周组织生物钟基因的影响

田红妮; 张二荃; 吕胜青; 黄河; 杨天德

doi:10.16016/j.1000-5404.201907166

您当前的位置：

首页 >

文章列表页 >

地氟醚对小鼠中枢及外周组织生物钟基因的影响

基础医学 | 更新时间：2026-01-09

- 地氟醚对小鼠中枢及外周组织生物钟基因的影响
- Effects of desflurane on central and peripheral clock genes in mice
- 第三军医大学学报 2019年41卷第23期页码：2328-2334
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81370210)
- DOI：10.16016/j.1000-5404.201907166
  中图分类号：
- 收稿：2019-07-20，
  
  纸质出版：2019-12-25
- 稿件说明：
移动端阅览
田红妮, 张二荃, 吕胜青, 等. 地氟醚对小鼠中枢及外周组织生物钟基因的影响[J]. 第三军医大学学报, 2019,41(23):2328-2334.

Hongni TIAN, Erquan ZHANG, Shengqing LYU, et al. Effects of desflurane on central and peripheral clock genes in mice[J]. Journal of Third Military Medical University, 2019, 41(23): 2328-2334.
田红妮, 张二荃, 吕胜青, 等. 地氟醚对小鼠中枢及外周组织生物钟基因的影响[J]. 第三军医大学学报, 2019,41(23):2328-2334. DOI： 10.16016/j.1000-5404.201907166.

Hongni TIAN, Erquan ZHANG, Shengqing LYU, et al. Effects of desflurane on central and peripheral clock genes in mice[J]. Journal of Third Military Medical University, 2019, 41(23): 2328-2334. DOI： 10.16016/j.1000-5404.201907166.

摘要

Abstract

Objective

To investigate the effect and mechanism of the anesthetic drug

desflurane

on central and peripheral clock genes in mice.

Methods

C57BL/6J mice were anesthetized with different concentrations (3%

9%) of desflurane

and then the effective concentration of desflurane was determined by detecting the loss of righting reflex. Then C57BL/6J mice and

Per1

-/-

Per

-/-

mice were respectively divided into air control group and desflurane treatment group. The mice from the latter group were given 7% desflurane treatment in 2 h after the light of feeding environment was turned on

and those of the control group was exposed to air at the same time. In 2 h later

the clock genes

Per1

Per2

Dbp

Bmal1

Clock

and

Cry1

in the hypothalamus

kidney and liver tissues were detected by RT-qPCR.

Results

① Treatment of 3% desflurane resulted in no disappearance of righting reflex

while those of 4%

7% and 9% caused the mice achieving loss of the righting reflex accounted for 16.7%

50%

100 % and 100%. Therefore

the effective concentration of desflurane was determined as 7%. ② Compared with the control group

treatment with 7% desflurane significantly decreased the expression of clock gene

Per2

in the hypothalamus tissue in C57BL/6J mice (

0.01)

but had no effect on the expression of the other

5 clock genes (

0.05); It also significantly increased the expression of the clock gene

Per1

while significantly decreased the expression of the clock genes

Bmal1

and

Dbp

in the kidney tissue (

0.05)

but there was no significant change in the expression of the other 3 clock genes (

0.05); In the liver tissue

the expression of clock gene

Per1

was significantly increased (

0.01)

while no changes were seen in the expression of the other 5 clock genes (

0.05). Compare with the control group

the

Per1

-/-

Per2

-/-

mice had significantly decreased the expression of

Bmal1

Clock

and

Dbp

in the hypothalamus tissue after treatment with 7% desflurane (

0.05); obviously reduced expression of

Cry1

and

Clock

(

0.05)

but no notable change in that of

Bmal1

(

0.05) in the liver and kidney tissue.

Conclusion

Desflurane significantly affects the circadian clock genes in mouse central and peripheral tissues

and there is a pathway independent form

Per

gene.

关键词

Keywords

references

TAKAHASHI J S Transcriptional architecture of the mammalian circadian clock Nat Rev Genet 2017 18 3 164 179 10.1038/nrg.2016.150 TAKAHASHI J S. Transcriptional architecture of the mammalian circadian clock[J]. Nat Rev Genet, 2017, 18(3): 164-179. DOI:10.1038/nrg.2016.150.

ZHANG E E KAY S A Clocks not winding down: unravelling circadian networks Nat Rev Mol Cell Biol 2010 11 11 764 776 10.1038/nrm2995 ZHANG E E, KAY S A. Clocks not winding down: unravelling circadian networks[J]. Nat Rev Mol Cell Biol, 2010, 11(11): 764-776. DOI:10.1038/nrm2995.

MAN K LOUDON A CHAWLA A Immunity around the clock Science 2016 354 6315 999 1003 10.1126/science.aah4966 MAN K, LOUDON A, CHAWLA A. Immunity around the clock[J]. Science, 2016, 354(6315): 999-1003. DOI:10.1126/science.aah4966.

ROENNEBERG T MERROW M The circadian clock and human health Curr Biol 2016 26 10 R432 R443 10.1016/j.cub.2016.04.011 ROENNEBERG T, MERROW M. The circadian clock and human health[J]. Curr Biol, 2016, 26(10): R432-R443. DOI:10.1016/j.cub.2016.04.011.

TAKAHASHI J S HONG H KO C H The genetics of mammalian circadian order and disorder: implications for physiology and disease Nat Rev Genet 2008 9 10 764 775 10.1038/nrg2430 TAKAHASHI J S, HONG H, KO C H, et al . The genetics of mammalian circadian order and disorder: implications for physiology and disease[J ] . Nat Rev Genet, 2008, 9(10): 764-775. DOI:10.1038/nrg2430.

MOHAWK J A GREEN C B TAKAHASHI J S Central and peripheral circadian clocks in mammals Annu Rev Neurosci 2012 35 445 462 10.1146/annurev-neuro-060909-153128 MOHAWK J A, GREEN C B, TAKAHASHI J S. Central and peripheral circadian clocks in mammals[J]. Annu Rev Neurosci, 2012, 35: 445-462. DOI:10.1146/annurev-neuro-060909-153128.

YAGITA K TAMANINI F VAN DER HORST G T Molecular mechanisms of the biological clock in cultured fibroblasts Science 2001 292 5515 278 281 10.1126/science.1059542 YAGITA K, TAMANINI F, VAN DER HORST G T, et al . Molecular mechanisms of the biological clock in cultured fibroblasts[J ] . Science, 2001, 292(5515): 278-281. DOI:10.1126/science.1059542.

BALSALOBRE A BROWN S A MARCACCI L Resetting of circadian time in peripheral tissues by glucocorticoid signaling Science 2000 289 5488 2344 2347 10.1126/science.289.5488.2344 BALSALOBRE A, BROWN S A, MARCACCI L, et al . Resetting of circadian time in peripheral tissues by glucocorticoid signaling[J ] . Science, 2000, 289(5488): 2344-2347. DOI:10.1126/science.289.5488.2344.

BARKAI N LEIBLER S Circadian clocks limited by noise Nature 2000 403 6767 267 268 10.1038/35002258 BARKAI N, LEIBLER S. Circadian clocks limited by noise[J]. Nature, 2000, 403(6767): 267-268. DOI:10.1038/35002258.

STOKKAN K A YAMAZAKI S TEI H Entrainment of the circadian clock in the liver by feeding Science 2001 291 5503 490 493 10.1126/science.291.5503.490 STOKKAN K A, YAMAZAKI S, TEI H, et al . Entrainment of the circadian clock in the liver by feeding[J ] . Science, 2001, 291(5503): 490-493. DOI:10.1126/science.291.5503.490.

OCMEN E ERDOST H A DURU L S Effect of day/night administration of three different inhalational anesthetics on melatonin levels in rats Kaohsiung J Med Sci 2016 32 6 302 305 10.1016/j.kjms.2016.04.016 OCMEN E, ERDOST H A, DURU L S, et al . Effect of day/night administration of three different inhalational anesthetics on melatonin levels in rats[J ] . Kaohsiung J Med Sci, 2016, 32(6): 302-305. DOI:10.1016/j.kjms.2016.04.016.

GELEGEN C MIRACCA G RAN M Z Excitatory pathways from the lateral habenula enable propofol-induced sedation Curr Biol 2018 28 4 580 587 10.1016/j.cub.2017.12.050 GELEGEN C, MIRACCA G, RAN M Z, et al . Excitatory pathways from the lateral habenula enable propofol-induced sedation[J ] . Curr Biol, 2018, 28(4): 580-587.e5. DOI:10.1016/j.cub.2017.12.050.

TAO G R XUE Q S LUO Y Isoflurane is more deleterious to developing brain than desflurane: the role of the akt/GSK3β signaling pathway Biomed Res Int 2016 2016 7919640 10.1155/2016/7919640 TAO G R, XUE Q S, LUO Y, et al . Isoflurane is more deleterious to developing brain than desflurane: the role of the akt/GSK3β signaling pathway[J ] . Biomed Res Int, 2016, 2016: 7919640. DOI:10.1155/2016/7919640.

XU K X TAO J ZHANG N Neuroprotective properties of vitamin C on equipotent anesthetic concentrations of desflurane, isoflurane, or sevoflurane in high fat diet fed neonatal mice Int J Clin Exp Med 2015 8 7 10444 10458 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1177/096032719701600706 XU K X, TAO J, ZHANG N, et al . Neuroprotective properties of vitamin C on equipotent anesthetic concentrations of desflurane, isoflurane, or sevoflurane in high fat diet fed neonatal mice[J ] . Int J Clin Exp Med, 2015, 8(7): 10444- 10458.

GILE J SCOTT B ECKLE T The period 2 enhancer nobiletin as novel therapy in murine models of circadian disruption resembling delirium Crit Care Med 2018 46 6 e600 e608 10.1097/CCM.0000000000003077 GILE J, SCOTT B, ECKLE T. The period 2 enhancer nobiletin as novel therapy in murine models of circadian disruption resembling delirium[J]. Crit Care Med, 2018, 46(6): e600-e608. DOI:10.1097/CCM.0000000000003077.

OHE Y ⅡJIMA N KADOTA K The general anesthetic sevoflurane affects the expression of clock gene m Per2 accompanying the change of NAD+ level in the suprachiasmatic nucleus of mice Neurosci Lett 2011 490 3 231 236 10.1016/j.neulet.2010.12.059 OHE Y, ⅡJIMA N, KADOTA K, et al . The general anesthetic sevoflurane affects the expression of clock gene m Per2 accompanying the change of NAD+ level in the suprachiasmatic nucleus of mice[J ] . Neurosci Lett, 2011, 490(3): 231-236. DOI:10.1016/j.neulet.2010.12.059.

YOSHIDA Y NAKAZATO K TAKEMORI K The influences of propofol and dexmedetomidine on circadian gene expression in rat brain Brain Res Bull 2009 79 6 441 444 10.1016/j.brainresbull.2009.04.015 YOSHIDA Y, NAKAZATO K, TAKEMORI K, et al . The influences of propofol and dexmedetomidine on circadian gene expression in rat brain[J ] . Brain Res Bull, 2009, 79(6): 441-444. DOI:10.1016/j.brainresbull.2009.04.015.

GUO H N BREWER J M CHAMPHEKAR A Differential control of peripheral circadian rhythms by suprachiasmatic-dependent neural signals Proc Natl Acad Sci U S A 2005 102 8 3111 3116 10.1073/pnas.0409734102 GUO H N, BREWER J M, CHAMPHEKAR A, et al . Differential control of peripheral circadian rhythms by suprachiasmatic-dependent neural signals[J ] . Proc Natl Acad Sci U S A, 2005, 102(8): 3111-3116. DOI:10.1073/pnas.0409734102.

TAKAHASHI J S Molecular components of the circadian clock in mammals Diabetes Obes Metab 2015 17 Suppl 1 6 11 10.1111/dom.12514 TAKAHASHI J S. Molecular components of the circadian clock in mammals[J]. Diabetes Obes Metab, 2015, 17(Suppl 1): 6-11. DOI:10.1111/dom.12514.

WANG T WANG Z YANG P PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice Cell Death Dis 2016 7 e2176 10.1038/cddis.2016.9 WANG T, WANG Z, YANG P, et al . PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice[J ] . Cell Death Dis, 2016, 7: e2176. DOI:10.1038/cddis.2016.9.

FIRSOV D BONNY O Circadian rhythms and the kidney Nat Rev Nephrol 2018 14 10 626 635 10.1038/s41581-018-0048-9 FIRSOV D, BONNY O. Circadian rhythms and the kidney[J]. Nat Rev Nephrol, 2018, 14(10): 626-635. DOI:10.1038/s41581-018-0048-9.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

代谢生物钟：昼夜节律调控代谢研究的最新进展