沉默NUPR1降低人多发性骨髓瘤U266细胞自噬并促进其迁移和侵袭

李星欣; 黎安茂; 曾沉思; 陈建斌

doi:10.16016/j.1000-5404.201907123

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沉默NUPR1降低人多发性骨髓瘤U266细胞自噬并促进其迁移和侵袭

基础医学 | 更新时间：2026-01-09

- 沉默NUPR1降低人多发性骨髓瘤U266细胞自噬并促进其迁移和侵袭
- Silencing NUPR1 down-regulates autophagy and promotes migration and invasion in human multiple myeloma U266 cells
- 第三军医大学学报 2019年41卷第23期页码：2322-2327
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201907123
  中图分类号：
- 收稿：2019-07-16，
  
  纸质出版：2019-12-25
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李星欣, 黎安茂, 曾沉思, 等. 沉默NUPR1降低人多发性骨髓瘤U266细胞自噬并促进其迁移和侵袭[J]. 第三军医大学学报, 2019,41(23):2322-2327.

Xingxin LI, Anmao LI, Chensi ZENG, et al. Silencing NUPR1 down-regulates autophagy and promotes migration and invasion in human multiple myeloma U266 cells[J]. Journal of Third Military Medical University, 2019, 41(23): 2322-2327.
李星欣, 黎安茂, 曾沉思, 等. 沉默NUPR1降低人多发性骨髓瘤U266细胞自噬并促进其迁移和侵袭[J]. 第三军医大学学报, 2019,41(23):2322-2327. DOI： 10.16016/j.1000-5404.201907123.

Xingxin LI, Anmao LI, Chensi ZENG, et al. Silencing NUPR1 down-regulates autophagy and promotes migration and invasion in human multiple myeloma U266 cells[J]. Journal of Third Military Medical University, 2019, 41(23): 2322-2327. DOI： 10.16016/j.1000-5404.201907123.

摘要

Abstract

Objective

To explore the effect of silencing nuclear protein 1 (NUPR1) on autophagy

migration and invasion in human multiple myeloma (MM) U266 cells and its possible mechanism.

Methods

NUPR1-shRNA was transfected into MM U266 cells

and the transfection rate was detected by flow cytometry. Then the cells were divided into negative control cells (transfected with unrelated sequence RNA lentiviral vector) and NUPR1 knockdown cells (transfected with NUPR1-shRNA lentiviral vector). The interference effect of NUPR1 was detected by qRT-PCR

and the expression levels of autophagy related proteins (Beclin1

ATG5

LC3Ⅱ/LC3Ⅰ

P62)

migration and invasion associated proteins (MMP9

CXCR4) and pathway proteins (p-AKT/T-AKT

p-mTOR/T-mTOR) were measured by Western blotting. Transmission electron microscopy (TEM) was used to observe autophagosomes. Transwell test was employed to detect cell migration and invasion.

Results

The expression of NUPR1 at mRNA and protein level was significantly decreased in NUPR1 knockdown cells (

0.05). The protein levels of autophagy related proteins

ATG5

Beclin1 and LC3Ⅱ/LC3Ⅰwere significantly lower

while those of f MMP9

CXCR4

P62

p-AKT/T-AKT and p-mTOR/T-mTOR were increased obviously in the NUPR1 knockdown cells than the negative control cells (

0.05). There were significantly reduced autophagosomes in NUPR1 knockdo

wn cells. While

migratory and invasive abilities were enhanced in NUPR1 knock down group

and rapamycin reduced the promoting effects.

Conclusion

Silencing NUPR1 may promotes migration and invasion by down-regulating autophagy in U266 cells

which may be related to activation of the AKT/mTOR pathway.

关键词

Keywords

references

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