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血清APE1蛋白在结直肠癌临床诊断中的作用及意义
Diagnostic value and significance of serum APE1 in colorectal cancer
- 第三军医大学学报 2020年42卷第1期 页码：88-93
- 作者机构：
400042 重庆，陆军军医大学(第三军医大学)大坪医院肿瘤中心
- 作者简介：
[戴楠，E-mail:dn400042@hotmail.com DAI Nan, E-mail:dn400042@hotmail.com
- 基金信息：第三军医大学科技成果转化基金(2013XZH01);陆军军医大学临床科研重大专项基金(2018XLC2016)
DOI：10.16016/j.1000-5404.201907069
中图分类号： R341;R730.43;R735.35收稿：2019-07-09，
修回：2019-11-01，
纸质出版：2020-01-15
- 稿件说明：
移动端阅览
杨宇馨, 何乐, 卢先锋, 等. 血清APE1蛋白在结直肠癌临床诊断中的作用及意义[J]. 第三军医大学学报, 2020,42(1):88-93.
Yuxin YANG, Le HE, Xianfeng LU, et al. Diagnostic value and significance of serum APE1 in colorectal cancer[J]. Journal of Third Military Medical University, 2020, 42(1): 88-93.
杨宇馨, 何乐, 卢先锋, 等. 血清APE1蛋白在结直肠癌临床诊断中的作用及意义[J]. 第三军医大学学报, 2020,42(1):88-93. DOI： 10.16016/j.1000-5404.201907069.
Yuxin YANG, Le HE, Xianfeng LU, et al. Diagnostic value and significance of serum APE1 in colorectal cancer[J]. Journal of Third Military Medical University, 2020, 42(1): 88-93. DOI： 10.16016/j.1000-5404.201907069.
摘要
目的
2
探讨结直肠癌患者血清APE1蛋白的表达及其在临床诊断中的价值。
方法
2
利用ELISA法检测本院2010年1月至2015年9月肿瘤中心收治的202例结直肠癌患者和健康体检中心242例健康对照人群血清APE1蛋白的表达水平，通过免疫组化确定相应患者肿瘤组织APE1蛋白的表达特征，同时采用C-12肿瘤蛋白芯片试剂盒检测上述人群CEA的表达水平。分析血清APE1蛋白与肿瘤组织APE1蛋白表达水平及年龄、性别、分期等临床指标的相关性，并绘制受试者工作特征曲线(receiver operating characteristic curve，ROC)评估血清APE1蛋白及CEA在结直肠癌诊断中的应用价值。
结果
2
202例结直肠癌患者中位血清APE1蛋白浓度为0.331(0.007~4.359)ng/mL，显著高于对照组0.09
1(0~1.374)ng/mL(
P
<
0.01)。Spearman相关分析显示血清APE1蛋白水平与肿瘤组织中APE1蛋白表达呈正相关(
r
=0.331，
P
=0.025)，而与年龄、性别、分期等指标不相关。ROC曲线显示血清APE1蛋白的AUC为0.799(95%
CI
=0.756~0.843)，灵敏度为76.20%，特异性为76.40%，血清CEA的AUC为0.736(95%
CI
=0.687~0.785)，其灵敏度为51.00%，特异性为99.60%。血清APE1蛋白与CEA联合后其AUC为0.857(95%
CI
=0.819~0.896)，灵敏度和特异性分别为72.30%和91.30%。
结论
2
结直肠癌患者血清APE1蛋白水平与肿瘤组织APE1表达呈正相关且较健康对照人群显著增高。血清APE1蛋白可与CEA联合作为结直肠癌辅助诊断的血清学标志物。
Abstract
Objective
2
To investigate the expression of serum apurinic/apyrimidinic endonuclease 1 (APE1) in colorectal cancer and its diagnostic value.
Methods
2
A total of 202 patients with identified colorectal cancer admitted in our center from January 2010 to September 2015 were recruited in this study
and another 242 healthy volunteers who took physical examination at the same period served as normal control. Their serum APE1 level was quantified by ELISA. The expression of APE1 protein in colorectal cancer tissues was detected by immunohistochemistry. The expression of carcinoembryonic antigen (CEA) was detected by C-12 tumor protein chip kit. The correlations of APE1 protein levels in the serum and tumor tissues with age
gender
clinical stage and other indicators were analyzed. Receiver operating characteristic (ROC) curve was plotted to evaluate the value of serum APE1 and CEA levels in the diagnosis of colorectal cancer.
Results
2
The serum APE1 level was 0.331 (0.007~4.359) ng/mL in the colorectal cancer patients
which was significantly higher than that in healthy control [0.091(0～1.374) ng/mL
P
<
0.001
]
. Spearman correlation analysis showed that the serum APE1 level was positively correlated with its protein expression in colorectal cancer tissues (
r
=0.331
P
=0.025)
but had no relatio
ns with age
sex and clinical stages. ROC curve displayed that the area under the curve (AUC) was 0.799 (95%
CI
=0.756~0.843)
the sensitivity was 76.20%
and the specificity was 76.40% for serum APE1 level in the diagnosis. While
the AUC value was 0.736 (95%
CI
=0.687~0.785)
the sensitivity was 51.00%
and the specificity was 99.60% for serum CEA level. Their combination increased the AUC value to 0.857 (95%
CI
=0.819~0.896)
and the sensitivity and accuracy to 72.30% and 91.30%
respectively.
Conclusion
2
The serum APE1 level is positively correlated with its expression level in the colorectal cancer tissue
and both are significantly higher than those in the health control. Combined detection of serum APE1 and CEA levels might be considered as serum markers in auxiliary diagnosis for colorectal cancer.
关键词
Keywords
references
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