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周细胞对脓毒症大鼠血管反应性的保护作用
Role and mechanism of pericyte in protection of vascular reactivity in septic rats
- 第三军医大学学报 2019年第21期 页码：2029-2034
- 作者机构：
400042 重庆，陆军军医大学(第三军医大学)野战外科研究所第二研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
[ 刘良明. E-mail:liangmingliu@yahoo.com LIU Liangming, E-mail:liangmingliu@yahoo.com
- 基金信息：国家自然科学基金重点项目(81830065)
DOI：10.16016/j.1000-5404.201907039
中图分类号： R322.13;R363.22;R631收稿：2019-07-05，
修回：2019-09-06，
纸质出版：2019-11-15
- 稿件说明：
移动端阅览
张紫森, 刘良明. 周细胞对脓毒症大鼠血管反应性的保护作用[J]. 第三军医大学学报, 2019,(21):2029-2034.
Zisen ZHANG, Liangming LIU. Role and mechanism of pericyte in protection of vascular reactivity in septic rats[J]. Journal of Third Military Medical University, 2019, (21): 2029-2034.
张紫森, 刘良明. 周细胞对脓毒症大鼠血管反应性的保护作用[J]. 第三军医大学学报, 2019,(21):2029-2034. DOI： 10.16016/j.1000-5404.201907039.
Zisen ZHANG, Liangming LIU. Role and mechanism of pericyte in protection of vascular reactivity in septic rats[J]. Journal of Third Military Medical University, 2019, (21): 2029-2034. DOI： 10.16016/j.1000-5404.201907039.
摘要
目的
2
观察周细胞(pericyte
PC)对脓毒症大鼠血管舒缩反应性的保护作用。
方法
2
采用盲肠结扎穿孔诱导大鼠脓毒症模型，将SD大鼠(12~14周龄，雌雄各半，体质量180~220 g)按随机单位设计方法分为4组(
n
=16):正常对照组、脓毒症组、常规治疗组(乳酸林格液体复苏+血管活性药多巴胺+头孢呋辛钠)、PC治疗组(PC 10
6
/只+常规治疗)，观察不同处理组大鼠肠系膜微动脉舒缩反应性、肠系膜微血管动静脉流速、肝肾血流量及肠系膜上动脉p-MLC
20
表达的变化。
结果
2
与正常对照组相比，脓毒症大鼠血管反应性明显降低(
P
<
0.01)；常规治疗可轻微改善脓毒症血管反应性；PC治疗后，脓毒症大鼠微动脉的舒缩反应性、组织器官的灌注量明显改善(
P
<
0.01)，肠系膜微动脉的收缩和舒张反应性分别增加至81.5%和86.9%。机制研究发现PC对脓毒症大鼠血管反应性的保护作用与血管p-MLC
20
有关(
P
<
0.01)。
结论
2
PC通过改善脓毒症大鼠血管收缩和舒张反应性从而实现了对整体器官的保护作用。
Abstract
Objective
2
To determine the protective effects of pericytes (PCs) on the mesenteric vasomotor reactivity in septic rats.
Methods
2
The sepsis model of rats was established with cecal ligation and puncture (CLP). SD rats (both sexes
12~14 weeks old
weighing 180 to 220 g) were randomly divided into normal control
sepsis
conventional treatment (CT group)
and PCs+CT group
with 16 rats in each group. The rats from the CT group were given conventional fluid resuscitation (Ringer lactate liquid)+dopamine+cefuroxime
and those of the PCs+CT group were given venous injection of 10
6
cells PCs in addition. The vascular reactivity in mesenteric arteriole
velocity of red blood cells in mesenteric arteriole and venule
amounts of liver and kidney blood flow
and expression level of p-MLC
20
in superior mesenteric artery were observed and compared among the groups.
Results
2
Compared with normal control group
the vascular reactivity was significantly decreased after septic shock (
P
<
0.01). Conventional treatment slightly improved the vascular reactivity. PCs treatment significantly improved vascular hyporeactivity and organ perfusion in rats with septic shock (
P
<
0.01)
with constriction and relaxation of mesenteric arteriole increased to 81.5% and 86.9%
respectively. The protective effect of PCs on vascular reactivity was associated with the expression of p-MLC
20
(
P
<
0.01).
Conclusion
2
PCs protect the vital organ function by improving the vascular constriction and relaxation in septic rats.
关键词
Keywords
references
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