PRR11在结直肠癌中的表达及其对结直肠癌侵袭和转移的影响

唐瑶; 向德兵; 王杰; 孙贵银

doi:10.16016/j.1000-5404.201907034

您当前的位置：

首页 >

文章列表页 >

PRR11在结直肠癌中的表达及其对结直肠癌侵袭和转移的影响

基础医学 | 更新时间：2026-01-09

- PRR11在结直肠癌中的表达及其对结直肠癌侵袭和转移的影响
- Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis
- 第三军医大学学报 2019年41卷第22期页码：2181-2189
- 作者机构：
  
  1.606000 四川泸州，西南医科大学附属医院肿瘤科
  2.402260 重庆，重庆市江津区中心医院肿瘤科
- 作者简介：
  
  [孙贵银，E-mail:sunriseok@126.comSUN Guiyin, E-mail: sunriseok@126.com
- 基金信息：
  
  重庆市卫生和计划生育委员会科研项目(2012-1-106)
- DOI：10.16016/j.1000-5404.201907034
  中图分类号： R341;R730.23;R735.35
- 收稿：2019-07-14，
  
  修回：2019-09-11，
  
  纸质出版：2019-11-30
- 稿件说明：
移动端阅览
唐瑶, 向德兵, 王杰, 等. PRR11在结直肠癌中的表达及其对结直肠癌侵袭和转移的影响[J]. 第三军医大学学报, 2019,41(22):2181-2189.

Yao TANG, Debing XIANG, Jie WANG, et al. Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis[J]. Journal of Third Military Medical University, 2019, 41(22): 2181-2189.
唐瑶, 向德兵, 王杰, 等. PRR11在结直肠癌中的表达及其对结直肠癌侵袭和转移的影响[J]. 第三军医大学学报, 2019,41(22):2181-2189. DOI： 10.16016/j.1000-5404.201907034.

Yao TANG, Debing XIANG, Jie WANG, et al. Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis[J]. Journal of Third Military Medical University, 2019, 41(22): 2181-2189. DOI： 10.16016/j.1000-5404.201907034.

摘要

目的

检测富含脯氨酸蛋白11(proline-rich protein 11，PRR11)在结直肠癌中的表达，探讨其对结直肠癌侵袭和转移的影响。

方法

应用免疫组织化学检测2015年1月至2018年3月重庆市江津区中心医院136例结直肠癌组织与68例癌旁组织中PRR11的表达；Western blot、RT-PCR及免疫荧光检测PRR11及EMT相关信号分子在结直肠癌细胞中的表达；细胞划痕实验、Transwell小室法检测细胞的迁移、侵袭能力。

结果

结直肠癌组织中PRR11阳性表达率高于癌旁组织(60.29%

1.47%

0.01)，且与结直肠癌的远处转移、瘤体大小、TNM分期有关(

0.05)；相比于正常结直肠上皮细胞，PRR11在结直肠癌细胞中高表达(

0.05)；过表达PRR11后，癌细胞HT29的迁移和侵袭能力增强，同时伴随明显的β-catenin核转位，且显著增强β-catenin和Vimentin的表达，抑制E-cadherin的表达(

0.05)；相反，沉默PRR11后，HT29的迁移和侵袭能力减弱，且显著抑制β-catenin和Vimentin的表达，增强E-cadherin的表达(

0.05)。

结论

PRR11在结直肠癌中高表达，且可能通过激活Wnt/β-catenin信号通路，促进β-catenin核转位，从而促进结直肠癌的侵袭和转移。

Abstract

Objective

To investigate the expression of proline-rich protein 11 (PRR11) in colorectal cancer and its role in regulating the invasion and metastasis of colorectal cancer.

Methods

The expression of PRR11 was detected using immunohistochemical SP method in 136 specimens of colorectal cancer tissues and 68 adjacent tissues collected from Jiangjin District Central Hospital between January

2015 and March

2018. We also examined the expression of PRR11 and epithelial-mesenchymal transition (EMT)-related signal molecules in 3 colorectal cancer cell lines (SW480

HT29 and HCT116) and normal human rectal epithelial cells (HCoEpiC) using Western blotting

RT-PCR and immunofluorescence assay; cell scratch assay and Transwell assay were used to compare the migration and invasion abilities of the colorectal cancer cells.

Results

The positive expression rate of PRR11 was significantly higher in colorectal cancer tissue than in the adjacent tissues (60.29%

1.47%

0.01). PRR11 expression was significantly correlated with tumor differentiation

distant metastasis

tumor size and tumor staging (

0.05). Compared with normal colorectal epithelial cells

the colorectal cancer cell lines expressed high levels of PRR11. Overexpression of PRR11 in HT29 cells significantly enhanced the cell migration and invasion ability

caused obvious β-catenin nuclear translocation

significantly up-regulated the expression of β-catenin and vimentin

and decreased the expression of E-cadherin (

0.05). Silencing PRR11 significantly lowered the migration and invasion ability of HT29 cells

decreased the expression of β-catenin and vimentin

and increased the expression of E-cadherin (

0.05).

Conclusion

PRR11 is highly expressed in colorectal cancer to promote tumor invasion and metastasis possibly by promoting the nuclear translocation of β-catenin

via

activating the Wnt/β-catenin signaling pathway.

关键词

Keywords

references

SIEGEL R L, MILLER K D, JEMAL A. Cancer statistics, 2018[J]. CA Cancer J Clin, 2018, 68(1): 7-30. DOI: 10.3322/caac.21442.

SIEGEL R, DESANTIS C, JEMAL A. Colorectal cancer statistics, 2014[J]. CA Cancer J Clin, 2014, 64(2): 104-117. DOI: 10.3322/caac.21220.

明畅畅.富含脯氨酸蛋白11和Ki-67在直肠癌中的表达及临床意义[D].福州: 福建医科大学, 2017.

MING C C. The expression and clinical significance of proline rich protein 11 and ki-67 in rectal cancer[D]. Fuzhou: Fujian Medical University, 2017.

李燕. PRR11和CTHRC1蛋白在结直肠癌组织中的表达情况及相关性研究[D].郑州: 郑州大学, 2019.

LI Y. Expressionand correlation of PRR11 and CTHRC1 in colorectal cancertissues[D]. Zhengzhou: Zhengzhou University, 2019.

HUANG Y J, NI R, WANG J, et al . Knockdown of lncRNA DLX6-AS1 inhibits cell proliferation, migration and invasion while promotes apoptosis by downregulating PRR11 expression and upregulating miR-144 in non-small cell lung cancer[J ] . Biomed Pharmacother, 2019, 109: 1851-1859. DOI: 10.1016/j.biopha.2018.09.151.

SONG Z C, LIU W Y, XIAO Y, et al . PRR11 is a prognostic marker and potential oncogene in patients with gastric cancer[J ] . PLoS ONE, 2015, 10(8): e0128943. DOI: 10.1371/journal.pone.0128943.

CHEN Y, CHA Z S, FANG W Z, et al . The prognostic potential and oncogenic effects of PRR11 expression inhilar cholangiocarcinoma[J ] . Oncotarget, 2015, 6(24): 20419-20433. DOI: 10.18632/oncotarget.3983.

张莲.非小细胞肺癌细胞中沉默PRR11基因抑制细胞增殖并诱发自噬的分子机理[D].重庆: 重庆医科大学, 2018.

ZHANG L. Silencing of PRR11 suppresses cell proliferation and induces autophagy in NSCLC cells[D]. Chongqing: Chongqing Medical University, 2018.

张莲, 张春冬, 卜友泉, 等. PRR11调节肺癌细胞系A549侵袭迁移的分子机制研究[J].重庆医科大学学报, 2018, 43(5): 662-666. DOI: 10.13406/j.cnki.cyxb.001692.

ZHANG L, ZHANG C D, BU Y Q, et al . Silencing PRR11 inhibits the migration and invasion of lung cancer A549 cells[J ] . J Chongqing Med Univ, 2018, 43(5): 662-666. DOI: 10.13406/j.cnki.cyxb.001692.

BRENNER H, KLOOR M, POX C P. Colorectalcancer[J]. Lancet, 2014, 383(9927): 1490-1502. DOI: 10.1016/s0140-6736(13)61649-9.

QI J J, YU Y, AKILLI ÖZTVRK Ö, et al . New Wnt/β-catenin target genes promote experimental metastasis and migration of colorectal cancer cells through different signals[J ] . Gut, 2016, 65(10): 1690-1701. DOI: 10.1136/gutjnl-2014-307900.

WANG Z Z, YANG J, DI J B, et al . Downregulated USP3 mRNA functions as a competitive endogenous RNA of SMAD4 by sponging miR-224 and promotes metastasis in colorectal cancer[J ] . Sci Rep, 2017, 7(1): 4281. DOI: 10.1038/s41598-017-04368-3.

ZHOU L, DENG Z Z, LI H Y, et al . Overexpression of PRR11 promotes tumorigenic capability and is associated with progression in esophageal squamous cell carcinoma[J ] . OncoTargets Ther, 2019, 12: 2677-2693. DOI: 10.2147/OTT.S180255.

ZHU J, HU H, WANG J, et al . PRR11 overexpression facilitates ovarian carcinoma cell proliferation, migration, and invasion through activation of the PI3K/AKT/β-catenin pathway[J ] . Cell Physiol Biochem, 2018, 49(2): 696-705. DOI: 10.1159/000493034.

QIAO W, WANG HY, ZHANG X Z, et al .Proline-rich protein 11 silencing inhibits hepatocellular carcinoma growth and epithelial-mesenchymal transition through β-catenin signaling[J ] . Gene, 2019, 681: 7-14. DOI: 10.1016/j.gene.2018.09.036.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

细胞外基质蛋白1在结直肠癌中的表达和临床意义

艾杜糖醛酸-2-硫酸酯酶与结直肠癌转移及预后的关系

抑制Tiam1基因表达促进EC9706细胞侵袭、转移和增强对5-FU化疗敏感性

miR-543/SNTB1轴调控结直肠癌免疫微环境

免疫治疗联合靶向治疗及化疗在微卫星稳定型转移性结直肠癌患者三线治疗中的疗效和安全性