准静态平面均匀流场条件下游离皮片体外培养效果评价

闫洪涛; 唐辉; 邱伟明; 宋林娟; 杨桂红; 伍津津

doi:10.16016/j.1000-5404.201907031

您当前的位置：

首页 >

文章列表页 >

准静态平面均匀流场条件下游离皮片体外培养效果评价

基础医学 | 更新时间：2026-01-09

- 准静态平面均匀流场条件下游离皮片体外培养效果评价
- Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field
- 第三军医大学学报 2019年41卷第23期页码：2335-2340
- 作者机构：
- 作者简介：
- 基金信息：
  
  全军后勤科研计划重大专项课题(AWSI4C003-4);国家自然科学基金面上项目(31600776)
- DOI：10.16016/j.1000-5404.201907031
  中图分类号：
- 收稿：2019-07-04，
  
  纸质出版：2019-12-25
- 稿件说明：
移动端阅览
闫洪涛, 唐辉, 邱伟明, 等. 准静态平面均匀流场条件下游离皮片体外培养效果评价[J]. 第三军医大学学报, 2019,41(23):2335-2340.

Hongtao YAN, Hui TANG, Weiming QIU, et al. Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field[J]. Journal of Third Military Medical University, 2019, 41(23): 2335-2340.
闫洪涛, 唐辉, 邱伟明, 等. 准静态平面均匀流场条件下游离皮片体外培养效果评价[J]. 第三军医大学学报, 2019,41(23):2335-2340. DOI： 10.16016/j.1000-5404.201907031.

Hongtao YAN, Hui TANG, Weiming QIU, et al. Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field[J]. Journal of Third Military Medical University, 2019, 41(23): 2335-2340. DOI： 10.16016/j.1000-5404.201907031.

摘要

Abstract

Objective

To observe and evaluate the effect of different perfusion frequencies on the survival of human skin specimens in a quasi-static planar homogeneous flow field.

Methods

The foreskin skin grafts in size of 4 mm were placed a quasi-static planar homogeneous flow field

and randomly divided into experimental group (perfused with high frequency) and the control group (perfused with low frequency). After 12 days' culture

PAS staining and VG staining were used to observe the changes of basement membrane

cell morphology and dermal collagen fibers. Moreover

the number of total cells and the apoptotic cells in the 2 groups were separately determined by HE staining and TUNEL assay. The dissolved oxygen concentration in culture medium was measured by a dissolved oxygen meter.

Results

In the low freq

uency perfusion group

gross observation showed characteristic features of necrosis; PAS and VG staining results indicated that the epidermis was severely separated

basement membrane structure disappeared and collagen fibers accumulated. Significant differences were seen in total cell number (249.35±13.00

37.50±9.18

0.001) and apoptotic rate between the experimental group and the control group. The dissolved oxygen level was higher in the former group than the latter group.

Conclusion

In the quasi-static planar homogeneous flow field

high frequency perfusion is suitable for the culture of free skin grafts

in vitro

关键词

Keywords

references

VARANI J PERONE P SPAHLINGER D M Human skin in organ culture and human skin cells (keratinocytes and fibroblasts) in monolayer culture for assessment of chemically induced skin damage Toxicol Pathol 2007 35 5 693 701 10.1080/01926230701481907 VARANI J, PERONE P, SPAHLINGER D M, et al . Human skin in organ culture and human skin cells (keratinocytes and fibroblasts) in monolayer culture for assessment of chemically induced skin damage[J ] . Toxicol Pathol, 2007, 35(5): 693- 701. DOI:10.1080/01926230701481907.

LIU H TUCHINDA P FISHELEVICH R Human in vitro skin organ culture as a model system for evaluating DNA repair J Dermatol Sci 2014 74 3 236 241 10.1016/j.jdermsci.2014.02.003 LIU H, TUCHINDA P, FISHELEVICH R, et al . Human in vitro skin organ culture as a model system for evaluating DNA repair[J ] . J Dermatol Sci, 2014, 74(3): 236-241. DOI:10.1016/j.jdermsci.2014.02.003.

伍津津 . 一种适用于三维组织细胞灌注培养的生物反应器: CN103966095A [P ] . 2014-08-06 . 伍津津.一种适用于三维组织细胞灌注培养的生物反应器: CN103966095A[P]. 2014-08-06.

WU J J . The bioreactor suitable for three-dimensional tissue perfusion culture: CN103966095A [P ] . 2014-08-06 . WU J J. The bioreactor suitable for three-dimensional tissue perfusion culture: CN103966095A[P]. 2014-08-06.

张维谭燃景唐辉三维培养条件下流体剪切力对人真皮成纤维细胞成骨分化的影响第三军医大学学报 2016 38 12 1416 1421 10.16016/j.1000-5404.201512010 http://aammt.tmmu.edu.cn/Upload/rhtml/201512010.htm 张维, 谭燃景, 唐辉, 等.三维培养条件下流体剪切力对人真皮成纤维细胞成骨分化的影响[J].第三军医大学学报, 2016, 38(12): 1416-1421. DOI:10.16016/j.1000-5404.201512010.

ZHANG W TAN R J TANG H Fluid shear stress affects osteogenic differentiation of human dermal fibroblasts in three-dimensional culture condition J Third Mil Med Univ 2016 38 12 1416 1421 10.16016/j.1000-5404.201512010 http://aammt.tmmu.edu.cn/Upload/rhtml/201512010.htm ZHANG W, TAN R J, TANG H, et al . Fluid shear stress affects osteogenic differentiation of human dermal fibroblasts in three-dimensional culture condition[J ] . J Third Mil Med Univ, 2016, 38(12): 1416-1421. DOI:10.16016/j.1000-5404.201512010.

唐辉闫洪涛陈年不同灌注流速对复方壳多糖组织工程真皮生长代谢的影响中华皮肤科杂志 2016 49 12 865 870 10.3760/cma.j.issn.0412-4030.2016.12.008 唐辉, 闫洪涛, 陈年, 等.不同灌注流速对复方壳多糖组织工程真皮生长代谢的影响[J].中华皮肤科杂志, 2016, 49(12): 865-870. DOI:10.3760/cma.j.issn.0412-4030.2016.12.008.

TANG H YAN H T CHEN N Effects of different medium perfusion rates on the growth and metabolism of composite chitosan-based tissue-engineered dermis Chin J Dermatol 2016 49 12 865 870 10.3760/cma.j.issn.0412-4030.2016.12.008.j.issn.0412-4030.2016.12.008 TANG H, YAN H T, CHEN N, et al . Effects of different medium perfusion rates on the growth and metabolism of composite chitosan-based tissue-engineered dermis[J ] . Chin J Dermatol, 2016, 49(12): 865-870. DOI:10.3760/cma.j.issn.0412-4030.2016.12.008.j.issn.0412-4030.2016.12.008.

YAN H T TANG H QIU W M A new dynamic culture device suitable for rat skin culture Cell Tissue Res 2019 375 3 723 731 10.1007/s00441-018-2945-4 YAN H T, TANG H, QIU W M, et al . A new dynamic culture device suitable for rat skin culture[J ] . Cell Tissue Res, 2019, 375(3): 723-731. DOI:10.1007/s00441-018-2945-4.

LUNI C SERENA E ELVASSORE N Human-on-chip for therapy development and fundamental science Curr Opin Biotechnol 2014 25 45 50 10.1016/j.copbio.2013.08.015 LUNI C, SERENA E, ELVASSORE N. Human-on-chip for therapy development and fundamental science[J]. Curr Opin Biotechnol, 2014, 25: 45-50. DOI:10.1016/j.copbio.2013.08.015.

ZHOU L J ZHANG X Q PAUS R The renaissance of human skin organ culture: A critical reappraisal Differentiation 2018 104 22 35 10.1016/j.diff.2018.10.002 ZHOU L J, ZHANG X Q, PAUS R, et al . The renaissance of human skin organ culture: A critical reappraisal[J ] . Differentiation, 2018, 104: 22-35. DOI:10.1016/j.diff.2018.10.002.

ARORA T MEHTA A K JOSHI V Substitute of animals in drug research: an approach towards fulfillment of 4R's Indian J Pharm Sci 2011 73 1 1 6 10.4103/0250-474X.89750 ARORA T, MEHTA A K, JOSHI V, et al . Substitute of animals in drug research: an approach towards fulfillment of 4R's[J ] . Indian J Pharm Sci, 2011, 73(1): 1-6. DOI:10.4103/0250-474X.8975 0.

VOLKMER E DROSSE I OTTO S Hypoxia in static and dynamic 3D culture systems for tissue engineering of bone Tissue Eng Part A 2008 14 8 1331 1340 10.1089/ten.tea.2007.0231 VOLKMER E, DROSSE I, OTTO S, et al . Hypoxia in static and dynamic 3D culture systems for tissue engineering of bone[J ] . Tissue Eng Part A, 2008, 14(8): 1331-1340. DOI:10.1089/ten.tea.2007.0231.

PIOLA M SONCINI M CANTINI M Design and functional testing of a multichamber perfusion platform for three-dimensional scaffolds Sci World J 2013 2013 123974 10.1155/2013/123974 PIOLA M, SONCINI M, CANTINI M, et al . Design and functional testing of a multichamber perfusion platform for three-dimensional scaffolds[J ] . Sci World J, 2013, 2013: 123974. DOI:10.1155/2013/123974.

ZHANG K ZHAO T HUANG X Dissolved oxygen concentration in the medium during cell culture:Defectsandimprovements Cell Biol Int 2016 40 3 354 360 10.1002/cbin.10570 ZHANG K, ZHAO T, HUANG X, et al . Dissolved oxygen concentration in the medium during cell culture:Defectsandimprovements[J ] . Cell Biol Int, 2016, 40(3): 354-360. DOI:10.1002/cbin.10570.

URBANI L PICCOLI M FRANZIN C Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential PLoS ONE 2012 7 11 10.1371/journal.pone.0049860 URBANI L, PICCOLI M, FRANZIN C, et al . Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential[J ] . PLoS ONE, 2012, 7(11): e49860. DOI:10.1371/journal.pone.0049860.

HU Y L DELAY M JAHANGIRI A Hypoxia-induced autophagy promotes tumor cell survival and adaptation to antiangiogenic treatment in glioblastoma Cancer Res 2012 72 7 1773 1783 10.1158/0008-5472.CAN-11-3831 HU Y L, DELAY M, JAHANGIRI A, et al . Hypoxia-induced autophagy promotes tumor cell survival and adaptation to antiangiogenic treatment in glioblastoma[J ] . Cancer Res, 2012, 72(7): 1773-1783. DOI:10.1158/0008-5472.CAN-11-3831.

KLESZCZYNSKI K FISCHER T W Development of a short-term human full-thickness skin organ culture model in vitro under serum-free conditions Arch Dermatol Res 2012 304 7 579 587 10.1007/s00403-012-1239-z KLESZCZYNSKI K, FISCHER T W. Development of a short-term human full-thickness skin organ culture model in vitro under serum-free conditions[J ] . Arch Dermatol Res, 2012, 304(7): 579-587. DOI:10.1007/s00403-012-1239-z.

LU Z HASSE S BODO E Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions Exp Dermatol 2007 16 1 37 44 10.1111/j.1600-0625.2006.00510.x LU Z, HASSE S, BODO E, et al . Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions[J ] . Exp Dermatol, 2007, 16(1): 37-44. DOI:10.1111/j.1600-0625.2006.00510.x.

ISHIKAWA J OSHIMA M IWASAKI F Hypothermic temperature effects on organ survival and restoration Sci Rep 2015 5 9563 10.1038/srep09563 ISHIKAWA J, OSHIMA M, IWASAKI F, et al . Hypothermic temperature effects on organ survival and restoration[J ] . Sci Rep, 2015, 5:9563. DOI:10.1038/srep09563.

GEORGIEV M I EIBL R ZHONG J J Hosting the plant cells in vitro : recent trends in bioreactors Appl Microbiol Biotechnol 2013 97 9 3787 3800 10.1007/s00253-013-4817-x GEORGIEV M I, EIBL R, ZHONG J J. Hosting the plant cells in vitro : recent trends in bioreactors[J ] . Appl Microbiol Biotechnol, 2013, 97(9): 3787-3800. DOI:10.1007/s00253-013-4817-x.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

巴马小型猪与人真皮的组织形态学和生物力学基本特征比较研究

胶联羊膜的组织形态、细胞相容性及抗拉力特性研究