miR-204-5p通过靶向HMGB1抑制甲状腺乳头状癌K1细胞增殖、侵袭和迁移

郝军舰; 郝丽娟

doi:10.16016/j.1000-5404.201903113

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miR-204-5p通过靶向HMGB1抑制甲状腺乳头状癌K1细胞增殖、侵袭和迁移

临床医学 | 更新时间：2026-01-09

- miR-204-5p通过靶向HMGB1抑制甲状腺乳头状癌K1细胞增殖、侵袭和迁移
- MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene
- 第三军医大学学报 2019年41卷第16期页码：1559-1565
- 作者机构：
  
  810000 西宁，青海红十字医院急诊外科
- 作者简介：
  
  [ 郝军舰, E-mail:641002095@qq.com HAO Junjian, E-mailE-mail:641002095@qq.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201903113
  中图分类号： R394.2;R730.23;R736.1
- 收稿：2019-03-14，
  
  修回：2019-05-30，
  
  纸质出版：2019-08-30
- 稿件说明：
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郝军舰, 郝丽娟. miR-204-5p通过靶向HMGB1抑制甲状腺乳头状癌K1细胞增殖、侵袭和迁移[J]. 第三军医大学学报, 2019,41(16):1559-1565.

Junjian HAO, Lijuan HAO. MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene[J]. Journal of Third Military Medical University, 2019, 41(16): 1559-1565.
郝军舰, 郝丽娟. miR-204-5p通过靶向HMGB1抑制甲状腺乳头状癌K1细胞增殖、侵袭和迁移[J]. 第三军医大学学报, 2019,41(16):1559-1565. DOI： 10.16016/j.1000-5404.201903113.

Junjian HAO, Lijuan HAO. MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene[J]. Journal of Third Military Medical University, 2019, 41(16): 1559-1565. DOI： 10.16016/j.1000-5404.201903113.

摘要

目的

探讨miR-204-5p对甲状腺乳头状癌K1细胞增殖、侵袭和迁移的影响及其机制。

方法

将体外培养的K1细胞分为miR-NC组(转染miR-204-5p mimics阴性对照)、miR-204-5p组(转染miR-204-5p mimics)、anti-miR-NC组(转染miR-204-5p inhibitor阴性对照)和anti-miR-204-5p组(转染miR-204-5p inhibitor)，RT-PCR检测各组细胞中miR-204-5p的表达，MTT法检测细胞增殖活力，Transwell小室实验检测细胞侵袭和迁移能力。双荧光素酶报告基因实验检测miR-204-5p与HMGB1的靶向关系，RT-PCR和Western blot检测miR-204-5p对HMGB1 mRNA和蛋白表达的调控作用。通过转染HMGB1干扰序列siRNA-HMGB1和HMGB1过表达质粒pcDNA3.1-HMGB1构建HMGB1低表达和过表达的K1细胞株，观察HMGB1对K1细胞增殖、侵袭和迁移的影响。

结果

与miR-NC组相比，miR-204-5p组细胞中miR-204-5p表达水平明显升高；而anti-miR-204-5p组细胞中miR-204-5p表达的水平较anti-miR-NC组明显降低(

0.05)。miR-204-5p组细胞的增殖活力、侵袭能力和迁移能力较miR-NC组均明显降低，而anti-miR-204-5p组较anti-miR-NC组均明显升高(

0.05)；HMGB1是miR-204-5p潜在的靶基因，miR-204-5p可负向调控HMGB1表达。HMGB1低表达可抑制K1细胞增殖、侵袭和迁移，而HMGB1过表达则促进K1细胞增殖、侵袭和迁移。

结论

miR-204-5p可靶向HMGB1抑制K1细胞增殖、侵袭和迁移。

Abstract

Objective

To investigate the effects of miR-204-5p on the proliferation

invasion and migration of thyroid papillary carcinoma K1 cells and its mechanism.

Methods

K1 cells cultured

in vitro

were transfected with a miR-204-5p mimics

a miR-204-5p inhibitor

or their respective negative controls (miR-NC and anti-miR-NC)

and the expression of miR-204-5p in the cells was detected using RT-PCR. The changes in cell proliferation were assessed with MTT assay

and the changes in cell invasion and migration were evaluated with Transwell assay. The targeting relationship between miR-204-5p and

HMGB1

gene was tested using dual luciferase reporter gene assay

and the regulation of miR-204-5p on the expressions of HMGB1 mRNA and protein was examined using RT-PCR and Western blotting. The changes in the proliferation

invasion and migration of K1 cells were observed after transfection of the cells with siRNA-HMGB1 or a HMGB1 overexpression plasmid.

Results

The expression level of miR-204-5p was significantly increased in the cells transfected with miR-204-5p mimics and s

ignificantly lowered in cells transfected with miR-204-5p inhibitor as compared with their respective controls (

0.05). Overexpression of miR-204-5p significantly attenuated while inhibition of miR-204-5p significantly enhanced the proliferation

invasion and migration abilities of the cells in comparison with their respective controls (

0.05).

HMGB1

was a potential target gene of miR-204-5p

and was negatively regulated by miR-204-5p. RNA interference of HMGB1 obviously inhibited K1 cell proliferation

invasion and migration

while overexpression of HMGB1 promoted the proliferation

invasion and migration of the cells.

Conclusion

Overexpression of miR-204-5p can inhibit the proliferation

invasion and migration of K1 cells by targeted down-regulation of HMGB1 expression.

关键词

Keywords

references

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