miR-382通过PGC-1α对三阴性乳腺癌细胞株4T1生物学特性的影响

雷优扬; 周华; 代朦; 靳鑫; 明佳

doi:10.16016/j.1000-5404.201903048

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miR-382通过PGC-1α对三阴性乳腺癌细胞株4T1生物学特性的影响

基础医学 | 更新时间：2026-01-09

- miR-382通过PGC-1α对三阴性乳腺癌细胞株4T1生物学特性的影响
- MiR-382 inhibits proliferation and migration of triple negative breast cancer cell line 4T1 by mediating PGC-1α
- 第三军医大学学报 2019年41卷第15期页码：1415-1422
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市卫生计生委医学科研计划项目(2016ZDXM012);重庆市科学技术委员会技术创新与应用示范(社会民生类，CSTC2018jscx-msybx0020);重庆市渝中区科技计划项目(20160104)
- DOI：10.16016/j.1000-5404.201903048
  中图分类号：
- 收稿：2019-03-06，
  
  纸质出版：2019-08-15
- 稿件说明：
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雷优扬, 周华, 代朦, 等. miR-382通过PGC-1α对三阴性乳腺癌细胞株4T1生物学特性的影响[J]. 第三军医大学学报, 2019,41(15):1415-1422.

Youyang LEI, Hua ZHOU, Meng DAI, et al. MiR-382 inhibits proliferation and migration of triple negative breast cancer cell line 4T1 by mediating PGC-1α[J]. Journal of Third Military Medical University, 2019, 41(15): 1415-1422.
雷优扬, 周华, 代朦, 等. miR-382通过PGC-1α对三阴性乳腺癌细胞株4T1生物学特性的影响[J]. 第三军医大学学报, 2019,41(15):1415-1422. DOI： 10.16016/j.1000-5404.201903048.

Youyang LEI, Hua ZHOU, Meng DAI, et al. MiR-382 inhibits proliferation and migration of triple negative breast cancer cell line 4T1 by mediating PGC-1α[J]. Journal of Third Military Medical University, 2019, 41(15): 1415-1422. DOI： 10.16016/j.1000-5404.201903048.

摘要

Abstract

Objective

To explore the effect of miR-382 down-regulating PGC-1α on the biological behaviors of triple negative breast cancer 4T1 cells.

Methods

① After miR-382 mimics or inhibitory probe anti-miR-382 was transfected into 4T1 cells

the expression level of miR-382 was detected by qRT-PCR. The changes of PGC-1α expression at mRNA and protein levels were detected by qRT-PCR and Western blotting

respectively. ② Luciferase reporter gene assay was adopted to identify the binding site of miR-382 mimics to PGC-1α 3'UTR. ③miR-382 mimics or control miR were co-transfected into 4T1 cells with pCDNA3.1 or pCDNA-PGC1α

respectively. Transwell migration assay

CCK8 assay and subcutaneous xenografts and tail vein lung xenografts were respectively used to detect the changes in cell invasiveness

cell proliferation

and tumor growth and lung metastasis in mice.

Results

The expression of PGC-1α was significantly decreased in 4T1 cells afte

r transfection with miR-382 mimics (

0.05)

and it was obviously increased after transfection with anti-miR-382 (

0.05). There was a targeted regulatory core sequence (ACAACTT) of miR-382 and PGC-1α

and the site was at PGC-1α 3'UTR 565-582. Compared with the control +pCDNA3.1 group

the cell migration and proliferation activity of the miR-382+pCDNA3.1 group was significantly decreased

and the size of the 4T1 subcutaneous tumor and the area of metastatic tumor in the lung were notably decreased (

0.05). Compared with the control +pCDNA3.1 group

the cell migration and proliferation activity were significantly increased in the miR-382+pCDNA-PGC-1α group

and the size of 4T1 subcutaneous tumor and the area of metastatic tumor in the lung was significantly increased (

0.05); The antitumor effect of miR-382 was blocked by overexpression of PGC-1α.

Conclusion

MiR-382 inhibits the proliferation and lung metastasis of 4T1 cells by down-regulating PGC-1α

and the interaction site may be PGC-1α 3'UTR 565-582.

关键词

Keywords

references

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