液相色谱-串联质谱法测定人血浆中伏立康唑和伏立康唑氮氧化物浓度及其临床应用

向荣凤; 尚圣兰; 熊丽蓉; 喻明洁; 戴青; 陈勇川

doi:10.16016/j.1000-5404.201902067

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液相色谱-串联质谱法测定人血浆中伏立康唑和伏立康唑氮氧化物浓度及其临床应用

临床医学 | 更新时间：2026-01-09

- 液相色谱-串联质谱法测定人血浆中伏立康唑和伏立康唑氮氧化物浓度及其临床应用
- Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application
- 第三军医大学学报 2019年41卷第15期页码：1478-1484
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市社会事业与民生保障科技创新专项项目(CSTC2017shmsA130043);陆军军医大学第一附属医院院管课题(SWH2014LC03)
- DOI：10.16016/j.1000-5404.201902067
  中图分类号：
- 收稿：2019-02-19，
  
  纸质出版：2019-08-15
- 稿件说明：
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向荣凤, 尚圣兰, 熊丽蓉, 等. 液相色谱-串联质谱法测定人血浆中伏立康唑和伏立康唑氮氧化物浓度及其临床应用[J]. 第三军医大学学报, 2019,41(15):1478-1484.

Rongfeng XIANG, Shenglan SHANG, Lirong XIONG, et al. Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application[J]. Journal of Third Military Medical University, 2019, 41(15): 1478-1484.
向荣凤, 尚圣兰, 熊丽蓉, 等. 液相色谱-串联质谱法测定人血浆中伏立康唑和伏立康唑氮氧化物浓度及其临床应用[J]. 第三军医大学学报, 2019,41(15):1478-1484. DOI： 10.16016/j.1000-5404.201902067.

Rongfeng XIANG, Shenglan SHANG, Lirong XIONG, et al. Determination of voriconazole and its N-oxide in human plasma by LC-MS/MS and its clinical application[J]. Journal of Third Military Medical University, 2019, 41(15): 1478-1484. DOI： 10.16016/j.1000-5404.201902067.

摘要

Abstract

Objective

To develop liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the content determination of voriconazole (VRCZ) and its N-oxide (VNO) in human plasma

and to apply it to clinical samples.

Methods

Ketoconazole was used as the internal standard (IS). The plasma samples were treated with methanol to precipitate protein and analyzed by HPLC-MS/MS. The detection was accomplished with electrospray ionization (ESI) operated in positive ionization mode

and ion reaction pairs were selected to be m/z350.1→m/z127.0 (VRCZ)

m/z366.1→m/z224.0 (VNO) and m/z531.0→m/z82.1 (Ketoconazole). Chromatographic separation had been achieved on Inertsil ODS-3 (50/2.1) with methanol-10 mmol/L ammonium acetate (90:10) as the mobile phase. Plasma concentrations of VRCZ and VNO in 23 patients were determined.

Results

Both VRCZ and VNO were linear (r>0.999) over the concentration ranges of 0.4~10.0 μg/mL

inter- and intra-batch relative standard deviations (RSDs) and matrix effect were all less than 15%. The 2 compounds remained stable during sample storage and handling progress. Plasma concentrations of VRCZ in 23 patients ranged from 0.4 to 14.3 μg/mL

and those of VNO ranged from 0.5 to 7.2 μg/mL. Significant variance was found among these patients.

Conclusion

LC-MS/MS is simple

quick and sensitive for detection of VRCZ and VNO

and can be used for drug monitoring in clinical practice.

关键词

Keywords

references

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