粪便细菌移植对非酒精性脂肪性肝病大鼠肝功能和脂代谢紊乱的影响

张宇; 王威巍; 左国华; 黄小兵; 李靖

doi:10.16016/j.1000-5404.201902012

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粪便细菌移植对非酒精性脂肪性肝病大鼠肝功能和脂代谢紊乱的影响

基础医学 | 更新时间：2026-01-09

- 粪便细菌移植对非酒精性脂肪性肝病大鼠肝功能和脂代谢紊乱的影响
- Effects of fecal microbiota transplantation on liver function and lipid metabolism disorder in rats with nonalcoholic fatty liver disease
- 第三军医大学学报 2019年41卷第13期页码：1200-1205
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院肝胆外科
- 作者简介：
  
  [李靖，E-mail: xqyylijing@tom.com LI Jing, E-mail: xqyylijing@tom.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201902012
  中图分类号： Q939.121;R459.9;R575.505
- 收稿：2019-02-07，
  
  修回：2019-04-27，
  
  纸质出版：2019-07-15
- 稿件说明：
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张宇, 王威巍, 左国华, 等. 粪便细菌移植对非酒精性脂肪性肝病大鼠肝功能和脂代谢紊乱的影响[J]. 第三军医大学学报, 2019,41(13):1200-1205.

Yu ZHANG, Weiwei WANG, Guohua ZUO, et al. Effects of fecal microbiota transplantation on liver function and lipid metabolism disorder in rats with nonalcoholic fatty liver disease[J]. Journal of Third Military Medical University, 2019, 41(13): 1200-1205.
张宇, 王威巍, 左国华, 等. 粪便细菌移植对非酒精性脂肪性肝病大鼠肝功能和脂代谢紊乱的影响[J]. 第三军医大学学报, 2019,41(13):1200-1205. DOI： 10.16016/j.1000-5404.201902012.

Yu ZHANG, Weiwei WANG, Guohua ZUO, et al. Effects of fecal microbiota transplantation on liver function and lipid metabolism disorder in rats with nonalcoholic fatty liver disease[J]. Journal of Third Military Medical University, 2019, 41(13): 1200-1205. DOI： 10.16016/j.1000-5404.201902012.

摘要

目的

建立大鼠非酒精性脂肪性肝病(nonalcoholic fatty liver disease，NAFLD)模型，检测FMT干预对模型大鼠肝功能和脂代谢的影响，初步探讨粪便细菌移植的临床意义。

方法

选择24只SD大鼠，分为4组，每组6只。正常对照组:正常饲料，0.2 mL/d生理盐水灌胃；其余3组采用高脂饲料喂养建立大鼠非酒精性脂肪肝模型；模型组:0.2 mL/d生理盐水灌胃；益生菌组:0.2 mL/d益生菌溶液灌胃(其中含益生菌2.7×10

)；FMT组:0.2 mL/d粪便细菌溶液灌胃(其中含粪便细菌2.7×10

)；持续8周。记录大鼠的生存情况，比较移植前后体质量、肝功能及血清胰岛素、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的变化。

结果

大鼠均未出现死亡；与模型组比较，FMT组粪便细菌移植后大鼠肝指数明显下降(

＜0.01)，肝功能明显好转，血浆丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)显著降低(

＜0.01)；与模型组比较，脂代谢紊乱在粪便细菌移植后得到明显改善，LDL胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(T-CHO)明显下降，高密度脂蛋白胆固醇(HDL-C)显著回升(

＜0.01)；空腹血糖及胰岛素抵抗指数在移植后明显下降(

＜0.01)；与模型组比较，FMT组大鼠炎症标志物IL-6和TNF-α显著降低(

＜0.01)。

结论

对大鼠进行人类粪便细菌移植是可行的；粪便细菌移植可以改善非酒精性脂肪性肝病大鼠的肝功能，纠正脂代谢紊乱。

Abstract

Objective

To establish a model of nonalcoholic fatty liver disease (NAFLD) in rats

and investigate the effects of fecal microbiota transplantation (FMT) intervention on liver function and lipid metabolism in model rats and explore its clinical significance.

Methods

A total of 24 SD rats were selected and a rat NAFLD model was established by high-fat diet feeding. The rats were divided into 4 groups

that is

group A (normal control group

0.2 mL/d normal saline)

group B (NAFLD model group

0.2 mL/d normal saline gavage)

group C (probiotics group

0.2 mL/d probiotic solution gavage

containing probiotics 2.7×10

) and group D (FMT group

0.2 mL/d fecal bacteria solution

intragastric administration of 2.7×10

fecal bacteria). After 8 weeks of treatment

the survival of the rats was recorded. The body weight

liver function

and changes in serum insulin

interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) before and after treatment were compared in the groups.

Results

There was no death in all the experimental rats. Compared with the model group

FMT induced significantly decrease in the liver index (

0.01)

improved liver function

and lower plasma ALT and AST levels (

0.01). Lipid metabolism disorder was also significantly improved after FMT intervention

including obviously decreased LDL cholesterol (LDL-C)

triglyceride (TG)

total cholesterol (T-CHO) and elevated high-density lipoprotein cholesterol (HDL-C) when compared with the levels in the model group (

0.01). Fasting blood glucose and insulin resistance index were also decreased significantly after the intervention (

0.01). What's more

serum contents of IL-6 and TNF-α were markedly lower in rats receiving FMT than those in the model group (

0.01).

Conclusion

Human FMT is feasible in rats. It can improve liver function and correct lipid metabolism disorder in NAFLD rats.

关键词

Keywords

references

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