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LAPTM4B在胶质瘤组织中表达及对细胞增殖和侵袭力的影响
Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion
- 第三军医大学学报 2019年41卷第15期 页码：1431-1437
- 作者机构：
- 作者简介：
- 基金信息：河南省科技攻关计划项目(192102310362)
DOI：10.16016/j.1000-5404.201902004
中图分类号：收稿：2019-02-02，
纸质出版：2019-08-15
- 稿件说明：
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赵岗, 张哲莹, 杜宝顺, 等. LAPTM4B在胶质瘤组织中表达及对细胞增殖和侵袭力的影响[J]. 第三军医大学学报, 2019,41(15):1431-1437.
Gang ZHAO, Zheying ZHANG, Baoshun DU, et al. Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion[J]. Journal of Third Military Medical University, 2019, 41(15): 1431-1437.
赵岗, 张哲莹, 杜宝顺, 等. LAPTM4B在胶质瘤组织中表达及对细胞增殖和侵袭力的影响[J]. 第三军医大学学报, 2019,41(15):1431-1437. DOI： 10.16016/j.1000-5404.201902004.
Gang ZHAO, Zheying ZHANG, Baoshun DU, et al. Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion[J]. Journal of Third Military Medical University, 2019, 41(15): 1431-1437. DOI： 10.16016/j.1000-5404.201902004.
摘要
Abstract
Objective
2
To detect the protein expression of lysosome-associated protein transmembrane-4 beta (LAPTM4B) in glioma tissues
and to determine its effect on cell proliferation and invasiveness and its correlation with prognosis.
Methods
2
A total of 86 glioma patients who underwent surgical resection in our hospital from June 2011 to June 2015 were recruited in this study
and they were followed up till June 30
2018. In the same period
25 cases of normal brain tissues were selected. Immunohistochemical assay (SP) was used to detect LAPTM4B protein expression in gliomas and normal brain tissues. Kaplan-Meier analysis and Log-Rank test were performed for survival analysis. Cox proportional hazards model analysis was carried out for risk factors affecting prognosis. U251 cells were cultured and divided into siRNA-LAPTM4B group
siRNA-NC group and blank group. The expression of LAPTM4B in the cells was detected by real-time fluorescent quantitative PCR. Cell proliferation was detected by MTT assay and colony formation assay. Cell migration and invasion was detected by Transwell assay. The protein levels of LAPTM4B
E-cadherin
N-cadherin and Vimentin were detected by Western blotting.
Results
2
The positive rate of LAPTM4B protein was 77.91% in glioma tissues
which was higher by 28.00% than that in the normal brain tissues (
P
<
0.001). The positive expression rate of LAPTM4B protein had statistical difference with different WHO grades (
P
<
0.05). Kaplan-Meier survival analysis showed that the median survival time and cumulative survival rate were significantly lower in the LAPTM4B-positive group than the negative group (
P
=0.002). Cox proportional hazard model analysis indicated that WHO classification and LAPTM4B protein expression were risk factors for prognosis of glioma patients (
P
<
0.05). Compared with the siRNA-NC group and the blank group
the relative expression of LAPTM4B at mRNA and protein levels were decreased in the siRNA-LAPTM4B group (
P
<
0.05)
and the cell proliferation at 24
48
72 and 96 h
the numbers of cellular clone
migrated cells and invasive cells
and protein levels of N-cadherin and Vimentin were decreased
while the level of E-cadherin protein was increased in the siRNA-LAPTM4B group (all
P
<
0.05).
Conclusion
2
LAPTM4B protein is highly expressed in glioma tissues
and promotes cell proliferation and invasion
which may be associated with epithelial-mesenchymal transition. Its expression level is correlated with prognosis of the patients.
关键词
Keywords
references
LAN F QING Q PAN Q Serum exosomal miR-301a as a potential diagnostic and prognostic biomarker for human glioma Cell Oncol (Dordr) 2018 41 1 25 33 10.1007/s13402-017-0355-3 LAN F, QING Q, PAN Q, et al .Serum exosomal miR-301a as a potential diagnostic and prognostic biomarker for human glioma[J ] .Cell Oncol (Dordr), 2018, 41(1):25-33.DOI:10.1007/s13402-017-0355-3.
LIPP E S MCLENDON R E Tissue is the issue:biomarkers of prognosis and classification in adult gliomas Semin Oncol Nurs 2018 34 5 430 442 10.1016/j.soncn.2018.10.002 LIPP E S, MCLENDON R E.Tissue is the issue:biomarkers of prognosis and classification in adult gliomas[J].Semin Oncol Nurs, 2018, 34(5):430-442.DOI:10.1016/j.soncn.2018.10.002.
LV J YANG H WANG X Decreased BRMS1L expression is correlated with glioma grade and predicts poor survival in glioblastoma via an invasive phenotype Cancer Biomark 2018 22 2 311 316 10.3233/CBM-171019 LV J, YANG H, WANG X, et al .Decreased BRMS1L expression is correlated with glioma grade and predicts poor survival in glioblastoma via an invasive phenotype[J ] .Cancer Biomark, 2018, 22(2):311-316.DOI:10.3233/CBM-171019.
MENG Y WANG L CHEN D LAPTM4B:an oncogene in various solid tumors and its functions Oncogene 2016 35 50 6359 6365 10.1038/onc.2016.189 MENG Y, WANG L, CHEN D, et al .LAPTM4B:an oncogene in various solid tumors and its functions[J ] .Oncogene, 2016, 35(50):6359-6365.DOI:10.1038/onc.2016.189.
DING H CHENG X DING N Association between LAPTM4B gene polymorphism and susceptibility to and prognosis of diffuse large B-cell lymphoma Oncol Lett 2018 15 1 264 270 10.3892/ol.2017.7318 DING H, CHENG X, DING N, et al .Association between LAPTM4B gene polymorphism and susceptibility to and prognosis of diffuse large B-cell lymphoma[J ] .Oncol Lett, 2018, 15(1):264-270.DOI:10.3892/ol.2017.7318.
张 静怡 单 丽珠 张 洁 LAPTM4B-35和MMP-9在胃癌中表达及临床意义 中国肿瘤临床 2017 44 24 1226 1231 10.3969/j.issn.1000-8179.2017.24.822 张静怡, 单丽珠, 张洁, 等.LAPTM4B-35和MMP-9在胃癌中表达及临床意义[J].中国肿瘤临床, 2017, 44(24):1226-1231.DOI:10.3969/j.issn.1000-8179.2017.24.822.
ZHANG J Y SHAN L Z ZHANG J Expression and significance of LAPTM4B-35 and MMP-9 in patients with gastric cancer Chin J Clin Oncol 2017 44 24 1226 1231 10.3969/j.issn.1000-8179.2017.24.822 ZHANG J Y, SHAN L Z, ZHANG J, et al .Expression and significance of LAPTM4B-35 and MMP-9 in patients with gastric cancer[J ] .Chin J Clin Oncol, 2017, 44(24):1226-1231.DOI:10.3969/j.issn.1000-8179.2017.24.822.
MENG F TAN S LIU T Predictive significance of combined LAPTM4B and VEGF expression in patients with cervical cancer Tumour Biol 2016 37 4 4849 4855 10.1007/s13277-015-4319-9 MENG F, TAN S, LIU T, et al .Predictive significance of combined LAPTM4B and VEGF expression in patients with cervical cancer[J ] .Tumour Biol, 2016, 37(4):4849-4855.DOI:10.1007/s13277-015-4319-9.
LOUIS D N OHGAKI H WIESTLER O D The 2007 WHO classification of tumours of the central nervous system Acta Neuropathol 2007 114 2 97 109 10.1007/s00401-007-0243-4 LOUIS D N, OHGAKI H, WIESTLER O D, et al .The 2007 WHO classification of tumours of the central nervous system[J ] .Acta Neuropathol, 2007, 114(2):97-109.DOI:10.1007/s00401-007-0243-4.
李 勐 刘 志慧 马 立新 趋化因子CCLl 8在胶质瘤中的表达及对其侵袭力影响的研究 中华神经医学杂志 2018 17 6 548 553 10.3760/cma.j.issn.1671-8925.2016.06.002 李勐, 刘志慧, 马立新, 等.趋化因子CCLl 8在胶质瘤中的表达及对其侵袭力影响的研究[J].中华神经医学杂志, 2018, 17(6):548-553.DOI:10.3760/cma.j.issn.1671-8925.2016.06.002.
LI M LIU Z H MA L X CCL18 is highly expressed in glioma tissues and promotes activity of glioblastoma ceils Chin J Neuromed 2018 17 6 548 553 10.3760/cma.j.issn.1671-8925.2016.06.002 LI M, LIU Z H, MA L X, et al .CCL18 is highly expressed in glioma tissues and promotes activity of glioblastoma ceils[J ] .Chin J Neuromed, 2018, 17(6):548-553.DOI:10.3760/cma.j.issn.1671-8925.2016.06.002.
YAMANAKA R HAYANO A KANAYAMA T Radiation-induced gliomas:a comprehensive review and meta-analysis Neurosurg Rev 2018 41 3 719 731 10.1007/s10143-016-0786-8 YAMANAKA R, HAYANO A, KANAYAMA T.Radiation-induced gliomas:a comprehensive review and meta-analysis[J].Neurosurg Rev, 2018, 41(3):719-731.DOI:10.1007/s10143-016-0786-8.
LU V M JUE T R MCDONALD K L The survival effect of repeat surgery at glioblastoma recurrence and its trend:A systematic review and meta-analysis World Neurosurg 2018 115 6 453 459 10.1016/j.wneu.2018.04.016 LU V M, JUE T R, MCDONALD K L, et al .The survival effect of repeat surgery at glioblastoma recurrence and its trend:A systematic review and meta-analysis[J ] .World Neurosurg, 2018, 115(6):453-459.e3.DOI:10.1016/j.wneu.2018.04.016.
孙 婷 靶向肿瘤的溶瘤腺病毒制备策略的研究进展 中国肿瘤生物治疗杂志 2018 25 2 198 205 10.3872/j.issn.1007-385x.2018.02.016 孙婷.靶向肿瘤的溶瘤腺病毒制备策略的研究进展[J].中国肿瘤生物治疗杂志, 2018, 25(2):198-205.DOI:10.3872/j.issn.1007-385x.2018.02.016.
SUN T Research progress of preparation strategy of oncolytic adenovirus for targeted cancer Chin J Cancer Biother 2018 25 2 198 205 10.3872/j.issn.1007-385x.2018.02.016 SUN T.Research progress of preparation strategy of oncolytic adenovirus for targeted cancer[J].Chin J Cancer Biother, 2018, 25(2):198-205.DOI:10.3872/j.issn.1007-385x.2018.02.016.
HASHEMI M BAHARI G TABASI F LAPTM4B gene polymorphism augments the risk of cancer:Evidence from an updated meta-analysis J Cell Mol Med 2018 22 12 6396 6400 10.1111/jcmm.13896 HASHEMI M, BAHARI G, TABASI F, et al .LAPTM4B gene polymorphism augments the risk of cancer:Evidence from an updated meta-analysis[J ] .J Cell Mol Med, 2018, 22(12):6396-6400.DOI:10.1111/jcmm.13896.
BLOM T LI S DICHLBERGER A LAPTM4B facilitates late endosomal ceramide export to control cell death pathways Nat Chem Biol 2015 11 10 799 806 10.1038/nchembio.1889 BLOM T, LI S, DICHLBERGER A, et al .LAPTM4B facilitates late endosomal ceramide export to control cell death pathways[J ] .Nat Chem Biol, 2015, 11(10):799-806.DOI:10.1038/nchembio.1889.
LI S WANG L MENG Y Increased levels of LAPTM4B, VEGF and survivin are correlated with tumor progression and poor prognosis in breast cancer patients Oncotarget 2017 8 25 41282 41293 10.18632/oncotarget.17176 LI S, WANG L, MENG Y, et al .Increased levels of LAPTM4B, VEGF and survivin are correlat ed with tumor progression and poor prognosis in breast cancer patients[J ] .Oncotarget, 2017, 8(25):41282-41293.DOI:10.18632/oncotarget.17176.
KONG F GAO F CHEN J Overexpressed LAPTM4B-35 is a risk factor for cancer recurrence and poor prognosis in non-small-cell lung cancer Oncotarget 2016 7 35 56193 56199 10.18632/oncotarget.10907 KONG F, GAO F, CHEN J, et al .Overexpressed LAPTM4B-35 is a risk factor for cancer recurrence and poor prognosis in non-small-cell lung cancer[J ] .Oncotarget, 2016, 7(35):56193-56199.DOI:10.18632/oncotarget.10907.
DONG X TAMURA K KOBAYASHI D LAPTM4B-35 is a novel prognostic factor for glioblastoma J Neurooncol 2017 132 2 295 303 10.1007/s11060-017-2369-0 DONG X, TAMURA K, KOBAYASHI D, et al .LAPTM4B-35 is a novel prognostic factor for glioblastoma[J ] .J Neurooncol, 2017, 132(2):295-303.DOI:10.1007/s11060-017-2369-0.
WANG Y SONG W KAN P Overexpression of Epsin 3 enhances migration and invasion of glioma cells by inducing epithelial mesenchymal transition Oncol Rep 2018 40 5 3049 3059 10.3892/or.2018.6691 WANG Y, SONG W, KAN P, et al .Overexpression of Epsin 3 enhances migration and invasion of glioma cells by inducing epithelial mesenchymal transition[J ] .Oncol Rep, 2018, 40(5):3049-3059.DOI:10.3892/or.2018.6691.
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