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跑步锻炼延缓APP/PS1双转基因小鼠内侧前额叶皮质树突棘数量的丢失
Running exercise reduces dendritic spine loss in medial prefrontal cortex of double transgenic APP/PS1 mice
- 第三军医大学学报 2019年41卷第14期 页码：1328-1335
- 作者机构：
400016 重庆, 重庆医科大学基础医学院组织胚胎学教研室, 干细胞与组织工程研究室
- 作者简介：
[吴宏，E-mail:wuhong6368@126.comWU Hong, E-mail:wuhong6368@126.com
- 基金信息：国家自然科学基金面上项目(816171259);国家自然科学基金面上项目(81871073);国家自然科学基金面上项目(81501156);国家自然科学基金面上项目(81501101)
DOI：10.16016/j.1000-5404.201901207
中图分类号： R338.26;R338.64;R339.4收稿：2019-01-25，
修回：2019-02-20，
纸质出版：2019-07-30
- 稿件说明：
移动端阅览
范金花, 周春妮, 蒋林, 等. 跑步锻炼延缓APP/PS1双转基因小鼠内侧前额叶皮质树突棘数量的丢失[J]. 第三军医大学学报, 2019,41(14):1328-1335.
Jinhua FAN, Chunni ZHOU, Lin JIANG, et al. Running exercise reduces dendritic spine loss in medial prefrontal cortex of double transgenic APP/PS1 mice[J]. Journal of Third Military Medical University, 2019, 41(14): 1328-1335.
范金花, 周春妮, 蒋林, 等. 跑步锻炼延缓APP/PS1双转基因小鼠内侧前额叶皮质树突棘数量的丢失[J]. 第三军医大学学报, 2019,41(14):1328-1335. DOI： 10.16016/j.1000-5404.201901207.
Jinhua FAN, Chunni ZHOU, Lin JIANG, et al. Running exercise reduces dendritic spine loss in medial prefrontal cortex of double transgenic APP/PS1 mice[J]. Journal of Third Military Medical University, 2019, 41(14): 1328-1335. DOI： 10.16016/j.1000-5404.201901207.
摘要
目的
2
运用无偏体视学方法精确定量研究跑步锻炼对中老年APP/PS1双转基因模型小鼠内侧前额叶皮质树突棘的作用。
方法
2
12月龄的雄性APP/ PS1转基因小鼠分为对照组和跑步组，并将同窝生野生型小鼠作为野生组，每组10只，共30只。对跑步组小鼠进行4个月的跑步锻炼；运用Morris水迷宫方法测试3组小鼠的空间学习记忆能力；运用无偏体视学方法精确定量3组小鼠内侧前额叶皮质树突棘的数量。
结果
2
在隐藏平台实验中，对照组平均逃避潜伏期明显大于跑步组和野生组(
F
=15.738，
P
＜0.001)，在空间探索实验中，3组小鼠穿台次数、目标象限游泳时间、目标象限游泳距离百分比差异都有统计学意义(
P
＜0.001);对照组内侧前额叶皮质的树突棘总数显著小于跑步组和野生组(
P
=0.029，
P
=0.005)。
结论
2
跑步锻炼可以延缓APP/PS1双转基因AD模型小鼠内侧前额叶皮质树突棘的丢失。
Abstract
Objective
2
To investigate the effect of running exercise on the number of dendritic spines in the medial prefrontal cortex in an APP/PS1 double transgenic mouse model of Alzheimer's disease (AD).
Methods
2
Twelve-month-old male APP/PS1 transgenic mice were randomly divided into control group and running exercise group (
n
=10)
and 10 wild-type littermates of the transgenic mice served as the wild-type control group. The mice in the running exercise group were subjected to forced running exercise for 4 months. The spatial learning and memory abilities of the mice were tested using Morris water maze test
and the total number of the dendritic spines in the medial prefrontal cortex was accurately determined using a unbiased stereological method.
Results
2
In the hidden platform task
the escape latency was significantly longer in the control group than in the wild-type control group and the running exercise group (
F
=15.738
P
<
0.001). In the probe task
the frequency of platform location crosses
the time spending in the target quadrant and the percentage distance in the target quadrant all differed significantly among the 3 groups (
P
<
0.001). The total number of dendritic spines in the medial prefrontal cortex was significantly smaller in the control group than in the running exercise group (
P
=0.029) and the wild-type control group (
P
=0.005).
Conclusion
2
Running exercise can reduce dendritic spine loss in the medial prefrontal cortex of APP/PS1 transgenic mouse models of AD.
关键词
Keywords
references
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