JIP1基因敲除对青年小鼠视网膜形态和突触蛋白表达的影响

张洁琼; 刘闻一; 林森; 叶剑

doi:10.16016/j.1000-5404.201901139

您当前的位置：

首页 >

文章列表页 >

JIP1基因敲除对青年小鼠视网膜形态和突触蛋白表达的影响

基础医学 | 更新时间：2026-01-09

- JIP1基因敲除对青年小鼠视网膜形态和突触蛋白表达的影响
- Effect of JIP1 knockout on retinal structure and synaptic protein expression in young mice
- 第三军医大学学报 2019年41卷第6期页码：563-569
- 作者机构：
  
  400042 重庆，陆军军医大学(第三军医大学)大坪医院眼科
- 作者简介：
  
  [叶剑，E-mail: yejian1979@163.com YE Jian, E-mail:yejian1979@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81570840)
- DOI：10.16016/j.1000-5404.201901139
  中图分类号： R322.91; R329.24; R394.2
- 收稿：2019-01-17，
  
  修回：2019-01-20，
  
  纸质出版：2019-03-30
- 稿件说明：
移动端阅览
张洁琼, 刘闻一, 林森, 等. JIP1基因敲除对青年小鼠视网膜形态和突触蛋白表达的影响[J]. 第三军医大学学报, 2019,41(6):563-569.

Jieqiong ZHANG, Wenyi LIU, SEN LIN, et al. Effect of JIP1 knockout on retinal structure and synaptic protein expression in young mice[J]. Journal of Third Military Medical University, 2019, 41(6): 563-569.
张洁琼, 刘闻一, 林森, 等. JIP1基因敲除对青年小鼠视网膜形态和突触蛋白表达的影响[J]. 第三军医大学学报, 2019,41(6):563-569. DOI： 10.16016/j.1000-5404.201901139.

Jieqiong ZHANG, Wenyi LIU, SEN LIN, et al. Effect of JIP1 knockout on retinal structure and synaptic protein expression in young mice[J]. Journal of Third Military Medical University, 2019, 41(6): 563-569. DOI： 10.16016/j.1000-5404.201901139.

摘要

目的

探讨JIP1基因对青年小鼠视网膜结构和突触蛋白表达的影响。

方法

选择1月龄JIP1基因敲除(JIP1-KO)雄鼠和野生型C57雄鼠各12只，通过HE染色观察1月龄野生型C57小鼠和JIP1-KO小鼠视网膜厚度变化，采用Western blot和免疫组化方法检测野生型C57小鼠和JIP1-KO小鼠视网膜神经节细胞标志物(neuronal class Ⅲ β-Tubulin

TUJ1)、神经丝蛋白(neurofilament

NF)和突触后密度蛋白95(postsynaptic density protein 95

PSD95)、突触素(synaptophysin

SYN)表达。

结果

1月龄野生型C57小鼠视网膜全层、外核层(outer nuclear layer，ONL)、内核层(inner nuclear layer，INL)厚度分别为(119.60±11.70)、(41.17±3.59)、(21.90±2.27)μm，JIP1-KO小鼠视网膜全层、ONL层、INL层厚度分别为(110.70±7.21)、(36.83±2.26)、(20.15±1.89)μm，各对应层之间厚度比较均无统计学差异(

0.05)；与野生型C57小鼠相比，JIP1-KO小鼠视网膜中TUJ1、PSD95、SYN表达水平均无统计学差异(

0.05)，TUJ1定位于视网膜神经节细胞层，神经细胞突起NF、PSD95、SYN定位于外网层。

结论

JIP1基因敲除对1月龄小鼠视网膜发育影响不显著。

Abstract

Objective

To investigate the role of JNK interacting protein 1 (JIP1) gene in retinal structure and synaptic protein expression in young mice.

Methods

Twelve JIP1 knockout (KO) mice (1 month old) were employed in this study. HE staining was used to observe the changes of retinal thickness in 1-month-old wild type C57 mice and JIP1-KO mice. Western blotting and immunohistochemical assay were used to detect the expression of retinal ganglion cells' markers neuronal class Ⅲ β-tubulin (TUJ1)

neurofilament (NF)

postsynaptic density protein 95 (PSD95) and synaptophysin (SYN) in the 2 groups.

Results

The thicknesses of the whole retinal layer

the inner nuclear layer (INL) and the outer nuclear layer (ONL) of 1 month old wild type C57 mice were 119.60±11.70

41.17±3.59 and 21.90±2.27 μm

respectively. And the thicknesses of the 3 layers of the JIP1-KO mice were 110.70±7.21

36.83±2.26 and 20.15±1.89 μm

respectively. There were no statistical differences in the thickness of the corresponding layers between the 2 kinds of mice (

0.05). Compared with the wild C57 mice

the expression levels of TUJ1

PSD95 and SYN in the retina of JIP1-KO mice had no significant difference (

0.05). TUJ1 was located in the retinal ganglion cell layer

while NF

PSD95 and SYN were located in the outer plexiform layer (OPL).

Conclusion

JIP1 knockout shows no obvious effect on the development of the retina in mice aged 1 month.

关键词

Keywords

references

KLINEDINST S, WANG X, XIONG X, et al . Independent pathways downstream of the Wnd/DLK MAPKKK regulate synaptic structure, axonal transport, and injury signaling[J ] . J Neurosci, 2013, 33(31): 12764-12778. DOI:10.1523/JNEUROSCI.5160-12.2013.

ZEKE A, MISHEVA M,REMENYI A, et al . JNK signaling: regulation and functions based on complex protein-protein partnerships[J ] . Microbiol Mol Biol Rev, 2016, 80(3): 793-835. DOI: 10.1128/MMBR.00043-14.

MOREL C, SHERRIN T, KENNEDDY N J, et al . JIP1-mediated JNK activation negatively regulates synaptic plasticity and spatial memory[J ] . J Neurosci, 2018, 37(11): 3708-3728.DOI:10.1523/JNEUROSCI.1913-17.2018.

CHIBA K, CHIEN K Y, SOBU Y, et al . Phosphorylation of KLC1 modifies interaction with JIP1 and abolishes the enhanced fast veloc ity of APP transport by kinesin-1[J ] . Mol Biol Cell, 2017, 28(26): 3857-3869. DOI:10.1091/mbc.E17-05-0303.

SUM T, LI Y, LI T, et al . JIP1 and JIP3 cooperate to mediate TrkB anterograde axonal transport by activating kinesin-1[J ] . Cell Mol Life Sci, 2017, 74(21): 4027-4044. DOI: 10.1007/s00018-017-2568-z.

DENG C Y, LEI W L, XU X H, et al . JIP1 mediates anterograde transport of Rab10 cargos during neuronal polarization[J ] . J Neurosci, 34(5): 1710-1723. DOI: 10.1523/JNEUROSCI.4496-13.2014.

JUNWON L, SANG H C, YOUNG B K, et al . Defined conditions for differentiation of functional retinal ganglion cells from human pluripotent stem cells[J ] . IOVS, 2018, 59(8): 3531-3545. DOI: 10.1167/iovs.17-23439.

MARGEVICIUS D R, BASTIAN C, FAN Q, et al . JNK-interacting protein 1 mediates Alzheimer's-like pathological features in AICD-transgenic mice[J ] . Neurobiol Aging, 2015, 36(8): 2370-2379. DOI: 10.1016/j.neurobiolaging.2015.04.013.

DAJASBAILADOR F, BANTOUNAS I, JONES E V, et al . Regulation of axon growth by the JIP1-AKT axis[J ] . J Cell Sci, 2014, 127(Pt 1): 230-239. DOI: 10.1242/jcs.137208.

WANG P, ZHANG J Y, RICHARDSON G P, et al . Two cytokine signaling molecules co-operate to promote axonal transport and growth[J ] . Exp Neurol, 2011, 228(2): 165-172. DOI: 10.1016/j.expneurol.2010.08.021.

BARNAT M, ENSLEN H, PROPST F, et al . Distinct roles of c-Jun N-terminal kinase isoforms in neurite initiation and elongation during axonal regeneration[J ] . J Neurosci, 2010, 30(23): 7804-7816.DOI:10.1523/JNEUROSCI.0372-10.2010.

DAJAS-BAILAFOR F, JONES E V, WHITMARSH A J. The JIP1 scaffold protein regulates axonal development in cortical neurons[J]. Curr Biol, 2008, 18(3): 221-226. DOI: 10.1016/j.cub.2008.01.025.

HEIN T W, WEN X, XIN X, et al . Acute and chronic hyperglycemia elicit JIP1/JNK-mediated endothelial vasodilator dysfunction of retinal arterioles[J ] . IOVS, 2016, 57(10): 4333-4343. DOI: 10.1167/iovs.16-19990.

ZHANG Y, QIU B, WANG J, et al . Effects of BDNF-transfected BMSCs on neural functional recovery and synaptophysin expression in rats with cerebral infarction[J ] . Mol Neurobiol, 2017, 54(5): 1-12. DOI: 10. 1007/s12035-016-9946-7.

LING Y Z, MA W, YU L, et al . Decreased PSD95 expression in medial prefrontal cortex (mPFC) was associated with cognitive impairment induced by sevoflurane anesthesia[J ] . J Zhejiang Univ Sci B, 2015, 16(9): 763-771. DOI: 10.1631/jzus.B1500006.

HAITAO W, MINFEI W, CHUANJUN Z, et al . Neurofilament proteins in axonal regeneration and neurodegenerative diseases[J ] . Neural Regen Res, 2012, 7(8): 620-626. DOI: 10.3969/j.issn.1673-5374.2012.08.010.

BRAUNGER B M, DEMMER C, TAMM E R. Oxygen Transport to Tissue ⅩⅩⅩⅢ[M]. New York: Springer Science Business Media, 2014: 9-13.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据