缺氧微环境下晚期糖基化终末产物对结肠癌HCT116细胞侵袭、迁移的影响

万林; 程勇; 王五艺; 隆姝孜; 刘洪良; 周丽

doi:10.16016/j.1000-5404.201901095

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缺氧微环境下晚期糖基化终末产物对结肠癌HCT116细胞侵袭、迁移的影响

基础医学 | 更新时间：2026-01-09

- 缺氧微环境下晚期糖基化终末产物对结肠癌HCT116细胞侵袭、迁移的影响
- Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment
- 第三军医大学学报 2019年41卷第15期页码：1453-1460
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201901095
  中图分类号：
- 收稿：2019-01-11，
  
  纸质出版：2019-08-15
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万林, 程勇, 王五艺, 等. 缺氧微环境下晚期糖基化终末产物对结肠癌HCT116细胞侵袭、迁移的影响[J]. 第三军医大学学报, 2019,41(15):1453-1460.

Lin WAN, Yong CHENG, Wuyi WANG, et al. Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment[J]. Journal of Third Military Medical University, 2019, 41(15): 1453-1460.
万林, 程勇, 王五艺, 等. 缺氧微环境下晚期糖基化终末产物对结肠癌HCT116细胞侵袭、迁移的影响[J]. 第三军医大学学报, 2019,41(15):1453-1460. DOI： 10.16016/j.1000-5404.201901095.

Lin WAN, Yong CHENG, Wuyi WANG, et al. Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment[J]. Journal of Third Military Medical University, 2019, 41(15): 1453-1460. DOI： 10.16016/j.1000-5404.201901095.

摘要

Abstract

Objective

To investigate the effects of advanced glycation end products (AGEs) on the proliferation

invasion

migration and epithelial-mesenchymal transition (EMT) of colon cancer HCT116 cells in hypoxic microenvironment.

Methods

The optimal AGEs concentration (200 μg/mL) and treatment time (48 h) fo

r promoting the proliferation of HCT116 cells were determined through a screening test with CCK-8 assay and used in subsequent experiment. HCT116 cells were treated with AGEs

hypoxia

or their combination

and the cell cycle changes were detected using flow cytometry; the changes in the invasive and migration abilities of the cells

in vitro

were assessed using Transwell chamber invasion and migration assays. The expressions of HIF-1α

RAGE

E-cadherin

N-cadherin

vimentin and MMP2 proteins in the treated cells were determined with Western blotting.

Results

Compared with the blank control cells

HCT116 cells exposed to a hypoxic microenvironment showed an increased proliferative activity

but AGEs alone did not significantly affect the cell proliferation. The cells treated with AGEs

hypoxia

and their combination all exhibited enhanced invasion and migration abilities compared with the blank control cells. The results of Western blotting showed that the expression level of HIF-1α

which was low in HCT116 cells in normoxic condition

was increased significantly in a hypoxic condition

and increased further after exposure of the cells to both AGEs and hypoxia. The expression of RAGE was increased after AGEs exposure of the cells in both normal and hypoxic environments

and the increase was more prominent in a hypoxic condition. Exposure to AGEs

hypoxia

and both all resulted in increased expressions of MMP2

N-cadherin and vimentin but lowered expression of E-cadherin in the cells.

Conclusion

AGEs can further enhance the invasive and migration abilities but has little effect on the proliferation of HCT116 cells exposed to a hypoxic environment.

关键词

Keywords

references

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