浏览全部资源
扫码关注微信
一氧化氮对红细胞携氧特性的作用及体内吸入对小鼠耐缺氧能力的影响
Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice
- 第三军医大学学报 2019年41卷第12期 页码：1131-1135
- 作者机构：
1.400016 重庆，重庆医科大学：基础医学院干细胞与组织工程研究室病理生理学教研室
2.400038 重庆，陆军军医大学(第三军医大学)高原军事医学系高原生理学与病理学教研室，特殊环境医学教育部重点实验室，全军高原医学重点实验
3.400016 重庆，重庆医科大学：实验教学管理中心
- 作者简介：
[沈宜，E-mail:shenyicq@163.com SHEN Yi, E-mail:shenyicq@163.com
- 基金信息：国家自然科学基金面上项目(81370150);国家自然科学基金面上项目(30973446);“十三五”全军后勤科研重点项目(BWS17J031);全军后勤科研“十二五”重大项目S(AWS14L005)
DOI：10.16016/j.1000-5404.201812238
中图分类号： R331.141;R364.4;R914.3收稿：2018-12-12，
修回：2019-02-12，
纸质出版：2019-06-30
- 稿件说明：
移动端阅览
苏文婷, 崔宇, 李晓栩, 等. 一氧化氮对红细胞携氧特性的作用及体内吸入对小鼠耐缺氧能力的影响[J]. 第三军医大学学报, 2019,41(12):1131-1135.
Wenting SU, Yu CUI, Xiaoxu LI, et al. Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice[J]. Journal of Third Military Medical University, 2019, 41(12): 1131-1135.
苏文婷, 崔宇, 李晓栩, 等. 一氧化氮对红细胞携氧特性的作用及体内吸入对小鼠耐缺氧能力的影响[J]. 第三军医大学学报, 2019,41(12):1131-1135. DOI： 10.16016/j.1000-5404.201812238.
Wenting SU, Yu CUI, Xiaoxu LI, et al. Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice[J]. Journal of Third Military Medical University, 2019, 41(12): 1131-1135. DOI： 10.16016/j.1000-5404.201812238.
摘要
目的
2
研究一氧化氮(nitric oxide
NO)对红细胞携氧特性和小鼠耐缺氧能力的影响及两者的相互关系。
方法
2
取8周龄C57雄性小鼠外周血，置于氧离曲线测绘仪的氧合池中，通入含有NO的氮气，描绘通入不同浓度NO时的氧离曲线，计算氧亲和力参数P
50
及希尔系数，探讨NO对小鼠外周血红细胞携氧特性的影响。小鼠按随机数字表法分为常氧组和缺氧组(5%O
2
)
缺氧组再根据未吸入NO及吸入NO浓度(5、10、20 mL/m
3
)分为4组。小鼠被麻醉后，将其口鼻部置于自制的密闭玻璃箱。玻璃箱预先通入纯N
2
、NO、15%O
2
，将气体浓度预置为5%O
2
和相应浓度的NO。用血氧饱和度仪检测鼠尾血氧饱和度，同时记录小鼠的存活时间。
结果
2
通入100、200 mL/m
3
NO均可使氧离曲线发生明显左移，显著降低P
50
(
P
<
0.05)。与常氧组比较，缺氧小鼠血氧饱和度显著降低，吸入20 mL/m
3
NO显著削弱小鼠在缺氧环境中血氧饱和度的降低程度(
P
<
0.05)。与未吸入NO的缺氧对照组比较，吸入5、10、20 mL/m
3
NO组小鼠存活时间均延长，其中10、20 mL/m
3
NO组小鼠存活时间的延长差异具有统计学意义(
P
<
0.05)。吸入20 mL/m
3
NO时，缺氧条件下的存活时间与血氧饱和度呈正相关关系(
P
<
0.05)。
结论
2
吸入NO增加细胞血红蛋白氧亲和力，改善缺氧条件下血氧饱和度；NO对红细胞携氧特性的改善作用与缺氧耐受力增强有关。
Abstract
Objective
2
To study the effects of nitric oxide (NO) inhalation on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice.
Methods
2
Peripheral blood from 8-week-old male mice was exposed to nitrogen gas containing NO in an oxygenation pool to generate the oxygen dissociation curve and calculate the oxygen affinity parameter P
50
and the Hill coefficient. Fifty 8-week-old male C57 mice were randomized into 5 groups (
n
=10) and subjected to inhalation of 21% O
2
5% O
2
or 5% O
2
with 5
10 or 20 mL/m
3
NO under anesthesia. The oxygen saturation in the tail vein blood of the mice was measured with an oximeter following the exposures
and the survival time of the mice was recorded.
Results
2
The introduction
of 100 and 200 mL/m
3
NO into the pooled whole blood resulted in a significant left shift of the oxygenation curve
and significantly decreased P
50
(
P
<
0.05) in a concentration-dependent manner. Hypoxic exposure of the mice caused a significant reduction in the oxygen saturation level in the tail vein blood
and this reduction was obviously attenuated by inhalation of 20 mL/m
3
NO (
P
<
0.05). Compared with hypoxia exposure
inhalation of 5
10
and 20 mL/m
3
NO concentration-dependently prolonged the survival time of the mice
and the effect was statistically significantly in mice inhaling 10 and 20 mL/m
3
NO (
P
<
0.05). In mice inhaling 20 mL/m
3
NO
the survival time under hypoxic conditions was positively correlated with the level of blood oxygen saturation (
P
<
0.05).
Conclusion
2
Inhalation of NO increases hemoglobin oxygen affinity and improves blood oxygen saturation in mice exposed to hypoxic conditions. NO enhances hypoxia tolerance by improving the oxygen-carrying capacity of the red blood cells.
关键词
Keywords
references
MIRZA S, CLAY R D, KOSLOW M A, et al. COPD guidelines: a review of the 2018 GOLD report[J]. Mayo Clin Proc, 2018, 93(10): 1488-1502. DOI: 10.1016/j.mayocp.2018.05.026.
HUANG Y Q, LIN D, TANIGUCHI C M. Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe?[J]. Sci China Life Sci, 2017, 60(10): 1114-1124. DOI: 10.1007/s11427-017-9178-y.
WILSON M H, NEWMAN S, IMRAY C H. The cerebral effects of ascent to high altitudes[J]. Lancet Neurol, 2009, 8(2): 175-191. DOI: 10.1016/S1474-4422(09)70014-6.
MONSALVE-NAHARRO J Á, DOMINGO-CHIVA E, GARCÍA CASTILLO S, et al. Inhaled nitric oxide in adult patients with acute respiratory distress syndrome[J]. Farm Hosp, 2017, 41(2): 292-312. DOI: 10.7399/fh.2017.41.2.10533.
CHEN J Y, YE Z X, WANG X F, et al. Nitric oxide bioavailability dysfunction involves in atherosclerosis[J]. Biomed Pharmacother, 2018, 97: 423-428. DOI: 10.1016/j.biopha.2017.10.122.
WILLIAMS B L, WIEBLER J M, LEE R E, et al. Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, Rana sylvatica[J]. J Comp Physiol B, 2018, 188(6): 957-966. DOI: 10.1007/s00360-018-1182-1.
UMBRELLO M, DYSON A, FEELISCH M, et al. The key role of nitric oxide in hypoxia: hypoxic vasodilation and energy supply-demand matching[J]. Antioxid Redox Signal, 2013, 19(14): 1690-1710. DOI: 10.1089/ars.2012.4979.
FAGO A, JENSEN F B. Hypoxia tolerance, nitric oxide, and nitrite: lessons from extreme animals[J]. Physiology (Bethesda), 2015, 30(2): 116-126. DOI: 10.1152/physiol.00051.2014.
BONAVENTURA C, FERRUZZI G, TESH S, et al. Effects of S-nitrosation on oxygen binding by normal and sickle cell hemoglobin[J]. J Biol Chem, 1999, 274(35): 24742-24748.
MARTINEZ-RUIZ R, MONTERO-HUERTA P, HROMI J, et al. Inhaled nitric oxide improves survival rates during hypoxia in a sickle cell (SAD) mouse model[J]. Anesthesiology, 2001, 94(6): 1113-1118.
STEPURO T L, ZINCHUK V V. Nitric oxide effect on the hemoglobin-oxygen affinity[J]. J Physiol Pharmacol, 2006, 57(1): 29-38.
GLADWIN M T, SHELHAMER J H, SCHECHTER A N, et al. Role of circulating nitrite and S-nitrosohemoglobin in the regulation of regional blood flow in humans[J]. Proc Natl Acad Sci U S A, 2000, 97(21): 11482-11487. DOI: 10.1073/pnas.97.21.11482.
PATEL R P, HOGG N, SPENCER N Y, et al. Biochemical characterization of human S-nitrosohemoglobin. Effects on oxygen binding and transnitrosation[J]. J Biol Chem, 1999, 274(22): 15487-15492.
JENSEN F B, HANSEN M N, MONTESANTI G, et al. Nitric oxide metabolites during anoxia and reoxygenation in the anoxia-tolerant vertebrate Trachemys scripta[J]. J Exp Biol, 2014, 217(3): 423-431. DOI: 10.1242/jeb.093179.
FILOGONIO R, JOYCE W, WANG T. Nitrergic cardiovascular regulation in the African lungfish, Protopterus aethiopicus[J]. Comp Biochem Physiol, Part A Mol Integr Physiol, 2017, 207: 52-56. DOI: 10.1016/j.cbpa.2016.12.030.
DURANSKI M R, GREER J J, DEJAM A, et al. Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver[J]. J Clin Invest, 2005, 115(5): 1232-1240. DOI: 10.1172/JCI22493.
MILLER S, TUDOR C, NYIMA, et al. Maternal and neonatal outcomes of hospital vaginal deliveries in Tibet[J]. Int J Gynaecol Obstet, 2007, 98(3): 217-221. DOI: 10.1016/j.ijgo.2007.03.033.
YANGZOM Y, QIAN L L, SHAN M N, et al. Outcome of hospital deliveries of women living at high altitude: a study from Lhasa in Tibet[J]. Acta Paediatr, 2008, 97(3): 317-321. DOI: 10.1111/j.1651-2227.2008.00628.x.
WU P P, SHANMINNA, LIANG K, et al. Exhaled nitric oxide is associated with postnatal adaptation to hypoxia in Tibetan and non-Tibetan newborn infants[J]. Acta Paediatr, 2016, 105(5): 475-482. DOI: 10.1111/apa.13331.
0
浏览量
0
下载量
0
CSCD
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
