MiR-107靶向Notch2促进胃癌细胞迁移和侵袭

余南荣; 曾祥; 徐厚巍; 蓝俊松

doi:10.16016/j.1000-5404.201812050

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MiR-107靶向Notch2促进胃癌细胞迁移和侵袭

基础医学 | 更新时间：2026-01-09

- MiR-107靶向Notch2促进胃癌细胞迁移和侵袭
- MicroRNA-107 promotes migration and invasion of gastric cancer cells by targeting Notch2
- 第三军医大学学报 2019年41卷第12期页码：1142-1148
- 作者机构：
  
  510095 广州广州医科大学附属肿瘤医院胃肠肿瘤外科
- 作者简介：
  
  [余南荣, E-mail: 13628635331@163.com YU Nanrong, E-mail: 13628635331@163.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201812050
  中图分类号： R394.3;R73-37;R735.2
- 收稿：2018-12-06，
  
  修回：2019-03-05，
  
  纸质出版：2019-06-30
- 稿件说明：
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余南荣, 曾祥, 徐厚巍, 等. MiR-107靶向Notch2促进胃癌细胞迁移和侵袭[J]. 第三军医大学学报, 2019,41(12):1142-1148.

Nanrong YU, Xiang ZENG, Houwei XU, et al. MicroRNA-107 promotes migration and invasion of gastric cancer cells by targeting Notch2[J]. Journal of Third Military Medical University, 2019, 41(12): 1142-1148.
余南荣, 曾祥, 徐厚巍, 等. MiR-107靶向Notch2促进胃癌细胞迁移和侵袭[J]. 第三军医大学学报, 2019,41(12):1142-1148. DOI： 10.16016/j.1000-5404.201812050.

Nanrong YU, Xiang ZENG, Houwei XU, et al. MicroRNA-107 promotes migration and invasion of gastric cancer cells by targeting Notch2[J]. Journal of Third Military Medical University, 2019, 41(12): 1142-1148. DOI： 10.16016/j.1000-5404.201812050.

摘要

目的

检测微小RNA-107(miR-107)在胃癌细胞中的表达

探讨其调控胃癌细胞迁移和侵袭的分子机制。

方法

采用Real-time PCR检测miR-107和Notch2在人胃癌细胞MKN45和人胃黏膜上皮细胞GES-1中的表达。转染miR-107 inhibitors至MKN45细胞

采用Transwell法检测MKN45细胞迁移和侵袭。转染Notch2过表达载体至MKN45细胞，采用Transwell、Real-time PCR、Weatern blot分别检测细胞迁移、侵袭和基质金属蛋白酶-9(matrix metalloproteinase-9，MMP-9)的表达。Targetscan在线预测和双荧光素酶报告基因实验验证miR-107和Notch2的靶向关系。Transwell实验检测抑制miR-107并沉默Notch2后MKN45细胞迁移和侵袭。

结果

与GES-1细胞比较，MKN45细胞中miR-107表达上调(

0.01)，而Notch2表达下调(

0.01)。抑制miR-107或过表达Notch2，则MKN45细胞迁移和侵袭数减少(

0.01)，过表达Notch2能促进MMP-9 mRNA和蛋白的表达(

0.01)。Targetscan在线预测和双荧光素酶报告基因实验结果表明：Notch2是miR-107的潜在靶基因，抑制miR-107可促进Notch2表达(

0.01)。在MKN45细胞中沉默Notch2

可逆转miR-107抑制剂对细胞迁移和侵袭的抑制作用。

结论

miR-107在胃癌细胞中表达上调

可通过靶向Notch2促进MMP-9表达

促进胃癌细胞迁移和侵袭。

Abstract

Objective

To detect the expression of microRNA-107 (miR-107) in gastric cancer cells and its molecular mechanism for regulating migration and invasion of gastric cancer cells.

Methods

The expression levels of miR-107 and Notch2 mRNA in gastric cancer MKN45 cells and gastric epithelial cells GES-1 were detected using real-time PCR. MKN45 cells were transfected with a miR-107 inhibitor and the changes in cell migration and invasion were detected with Transwell assay. In MKN45 cells transfected with a Notch2-overexpressing plasmid

the changes in cell migration and invasion and the expression of matrix metalloproteinase-9 (MMP-9) were detected using Transwell assay

real-time PCR and Western blotting

respectively. Targetscan online prediction and dual-luciferase assay were used to analyze the relationship between miR-107 and Notch2. The changes in the migration and invasion of MKN45 cells after inhibiting miR-107 and Notch2 silencing were investigated using Transwell assay.

Results

Compared with those in GES-1 cells

the expression of miR-107 was significantly up-regulated (

0.01) and Notch2 was significantly down-regulated (

0.01) in MKN45 cells. Both inhibition of miR-107 and overexpression of Notch2 significantly attenuated the migration and invasion abilities of MKN45 cells (

0.01). Overexpression of Notch2 significantly promoted the expressions of MMP-9 mRNA and protein in the cells (

0.01). The results of Targetscan online prediction and dual-luciferase assay showed that Notch2 was potentially the target gene of miR-107 (

0.01)

and inhibiting miR-107 promoted the expression of Notch2. Silencing Notch2 in MKN45 cells obviously reversed miR-107-induced inhibition of cell migration and invasion.

Conclusion

The expression of miR-107 is up-regulated in gastric cancer cells. MiR-107 enhances the expression of MMP-9 by targeting Notch-2 to promote the migration and invasion of gastric cancer cells.

关键词

Keywords

references

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