GCNT3在结直肠癌中的表达及临床意义

刘陕西; 叶钧; 尚杨杨; 陈颢元; 汪荣泉

doi:10.16016/j.1000-5404.201812043

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GCNT3在结直肠癌中的表达及临床意义

基础医学 | 更新时间：2026-01-09

- GCNT3在结直肠癌中的表达及临床意义
- Clinical Significance of GCNT3 in colorectal cancer
- 第三军医大学学报 2019年41卷第10期页码：913-917
- 作者机构：
  
  重庆，陆军军医大学西南医院全军消化病研究所
- 作者简介：
  
  [ 汪荣泉，E-mail：rongquanw@hotmail.com WANG Rongquan, E-mail:rongquanw@hotmail.com
- 基金信息：
  
  国家自然科学基金面上项目(81572903)
- DOI：10.16016/j.1000-5404.201812043
  中图分类号： R574.6;R735.3
- 收稿：2018-12-05，
  
  修回：2019-01-28，
  
  纸质出版：2019-05-30
- 稿件说明：
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刘陕西, 叶钧, 尚杨杨, 等. GCNT3在结直肠癌中的表达及临床意义[J]. 第三军医大学学报, 2019,41(10):913-917.

Shanxi LIU, Jun YE, Yangyang SHANG, et al. Clinical Significance of GCNT3 in colorectal cancer[J]. Journal of Third Military Medical University, 2019, 41(10): 913-917.
刘陕西, 叶钧, 尚杨杨, 等. GCNT3在结直肠癌中的表达及临床意义[J]. 第三军医大学学报, 2019,41(10):913-917. DOI： 10.16016/j.1000-5404.201812043.

Shanxi LIU, Jun YE, Yangyang SHANG, et al. Clinical Significance of GCNT3 in colorectal cancer[J]. Journal of Third Military Medical University, 2019, 41(10): 913-917. DOI： 10.16016/j.1000-5404.201812043.

摘要

目的

探讨GCNT3在结直肠癌中表达的水平变化及其对结直肠癌患者预后的影响。

方法

国际癌症和肿瘤基因数据库(TCGA)中下载结直肠癌患者GCNT3基因(编码C2GnT-2)的mRNA表达数据及临床信息，整理分析其表达水平变化对结直肠癌临床相关性及预后的影响，并通过细胞增殖实验观察干扰C2GnT-2合成后结直肠癌细胞增殖能力的变化情况。

结果

GCNT3在癌组织中较癌旁组织低表达(

0.001)；GCNT3低表达组病人无病生存率显著低于高表达组(

0.05)；干扰C2GnT-2合成后结直肠癌细胞增殖能力显著增强(

0.01)。

结论

GCNT3在结直肠癌中低表达，其低表达可能提示直肠癌患者无病生存率低。

Abstract

Objective

To investigate the expression level of GCNT3

a glycosyltransferase enzyme

in colorectal cancer (CRC) and its association with the prognosis of the patients.

Methods

The mRNA expression data of GCNT3 gene (encoding mucin-type core 2 synthase enzyme

C2GnT-2) and clinical information of the patients were obtained from the Cancer Genome Atlas database (TCGA). The correlation of GCNT3 mRNA expression level with the clinical characteristics of the patients was analyzed. Kaplan-Meier analysis and Cox regression analysis were used for assessing the prognostic value of GCNT3. We also tested the effect of a short hairpin RNA (shRNA) targeting GCNT3 on the proliferation of the colorectal cancer cell line HT-29 using MTT assay.

Results

GCNT3 mRNA expression level was significantly decreased in CRC tissues as compared with the paired adjacent colorectal tissues (

0.001). A low expression level of GCNT3 was significantly correlated with a reduced disease-free survival (DFS) rate in the patients with CRC (

0.05). In HT-29 cells

interference of C2GnT-2 with the specific shRNA significantly promoted the cell proliferation as compared with the control cells (

0.01).

Conclusion

The expression of GCNT3 is significantly downregulated in CRC tissues

and a low expression of GCNT3 can be indicative of a low disease-free survival rate of patients with CRC.

关键词

Keywords

references

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