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小鼠铜绿假单胞菌气管插管滴注法肺部感染模型的建立及评价
Establishment and evaluation of a mouse model of pulmonary
Pseudomonas aeruginosa infection by intratracheal instillation- 第三军医大学学报 2019年41卷第10期 页码：918-922
- 作者机构：
1.400038 重庆，陆军军医大学(第三军医大学)：第一附属医院药学部
2.400038 重庆，陆军军医大学(第三军医大学)：基础部化学教研室
3.056000 河北 邯郸，解放军980医院邯郸分院药剂科
- 作者简介：
[ 孙凤军，E-mail: fengj_sun@163.com SUN Fengjun, E-mail: fengj_sun@163.com
- 基金信息：国家自然科学基金青年科学基金项目(81301397)
DOI：10.16016/j.1000-5404.201811157
中图分类号： R378;R459.7;R563.1收稿：2018-11-22，
修回：2019-01-08，
纸质出版：2019-05-30
- 稿件说明：
移动端阅览
王瑜, 张定林, 冯伟, 等. 小鼠铜绿假单胞菌气管插管滴注法肺部感染模型的建立及评价[J]. 第三军医大学学报, 2019,41(10):918-922.
Yu WANG, Dinglin ZHANG, Wei FENG, et al. Establishment and evaluation of a mouse model of pulmonary
Pseudomonas aeruginosa infection by intratracheal instillation[J]. Journal of Third Military Medical University, 2019, 41(10): 918-922.王瑜, 张定林, 冯伟, 等. 小鼠铜绿假单胞菌气管插管滴注法肺部感染模型的建立及评价[J]. 第三军医大学学报, 2019,41(10):918-922. DOI： 10.16016/j.1000-5404.201811157.
Yu WANG, Dinglin ZHANG, Wei FENG, et al. Establishment and evaluation of a mouse model of pulmonary
Pseudomonas aeruginosa infection by intratracheal instillation[J]. Journal of Third Military Medical University, 2019, 41(10): 918-922. DOI： 10.16016/j.1000-5404.201811157.
摘要
目的
2
建立小鼠铜绿假单胞菌(Pseudomonas aeruginosa
PA)肺部感染模型，并对该模型进行初步评价。
方法
2
采用一种快速无创的小鼠气管插管滴注法，将小鼠在透射灯照射下经口咽气管插管，滴注1×10
8
CFU/mL的铜绿假单胞菌悬液50 μL，待接种感染7 d后处死，进行肺部菌落计数和病理学评价。
结果
2
经气管接种感染后，与对照组小鼠比较，感染组小鼠生理状态不佳，肺组织匀浆检出PA，血液中白细胞和中性粒细胞数目显著增多(
P
<
0.05)，且出现肺部病理学改变，显示有炎症反应；对照组小鼠肺组织匀浆则未见PA生长，且血液中白细胞和中性粒细胞数目正常，未出现炎症反应。
结论
2
采用小鼠气管插管滴注法，通过呼吸道感染途径可成功建立小鼠肺部PA感染模型，该模型具有稳定性好，易定量的优点。
Abstract
Objective
2
To establish and validate a mouse model of pulmonary infection of
Pseudomonas aeruginosa
.
Methods
2
The models of pulmonary
Pseudomonas aeruginosa
infection was established in mice by intratracheal instillation of 50 μL (1×10
8
CFU/mL)
Pseudomonas aeruginosa
suspension delivered through orotracheal intubation under a transillumination lamp. At 7 d after the infection
the mice were sacrificed for counting the bacterial colonies in the lungs and for pathological evaluation.
Results
2
After intratracheal instillation of
Pseudomonas aeruginosa
the mice showed a poor general condition and had positive findings of
Pseudomonas aeruginosa
in the lung tissues with significantly increased blood white blood cell and neutrophil counts compared with the control mice (
P
<
0.05); pathological examination revealed obvious pathological changes and inflammations in the lungs of the mice after the infection. In the control mice
no bacteria or path
ological changes were detected in the lung tissue
and the white blood cell and neutrophil counts were normal without evidence of inflammatory responses.
Conclusion
2
We successfully established a mouse model of pulmonary
Pseudomonas aeruginosa
infection by delivering the pathogens through tracheal intubation. This model shows a good stability and allows convenient quantitative analysis.
关键词
Keywords
references
徐辉, 何晓静, 李晓冰, 等.铜绿假单胞菌慢性肺部感染合并免疫低下大鼠模型的建立.中国医院药学杂志, 2017, 37(24): 2428-2431. DOI: 10.13286/j.cnki.chinhosppharmacyj.2017.24.03.
XUH, HE X J, LI X B, et al . Establishment of rat models of immunosuppression combined with chronic pulmonary infection of Pseudomonas aeruginosa. Chin Hosp Pharm J, 2017, 37(24): 2428-2431. DOI: 10.13286/j.cnki.chinhosppharmacyj.2017.24.03.
MERCHANT Z, BUCKTON G, TAYLOR K M, et al , A new era of pulmonary delivery of nano-antimicrobial therapeutics to treat chronic pulmonary infections. Curr Pharm Design, 2016, 22(17): 2577-2598.
MIYOSHI-AKIYAMA T, TADA T, OHMAGARI N, et al . Emergence and spread of epidemic multidrug-resistant Pseudomonas aeruginosa.Genome Biol Evol, 2017, 9(12): 3238-3245. DOI: 10.1093/gbe/evx243.
杨青梅, 张苹, 沈策.铜绿假单胞菌慢性肺部感染模型的建立及评价.中国呼吸与危重监护杂志, 2008, 7(1): 40-42, 56, 80. DOI: 10.3969/j.issn.1671-6205.2008.01.011.
YANG Q M, ZHANG P, SHEN C. Establishment and evaluation of a chronic pulmonary infection model due to Pseudomonas aeruginosa. Chin J Resp Crit Care Med, 2008, 7(1): 40-42, 56, 80. DOI: 10.3969/j.issn.1671-6205.2008.01.011.
LI Z, CLEMENS D L, LEE B Y, et al . Mesoporous silica nanoparticles with pH-sensitive nanovalves for delivery of moxifloxacin provide improved treatment of lethal pneumonic tularemia. ACS Nano, 2015, 9(11): 10778-10789. DOI: 10.1021/acsnano.5b04306.
宋方, 吕欢, 何晓静, 等.大鼠铜绿假单胞菌慢性肺部感染模型的建立与评价.中国医院药学杂志, 2015, 35(9): 786-789. DOI: 10.13286/j.cnki.chinhosppharmacyj.2015.09.06.
SONG F, LYU H, HE X J, et al . Establishment and evaluation of rat models with chronic pulmonary infection of Pseudomonas aeruginosa. Chin Hosp Pharm J, 2015, 35(9): 786-789. DOI: 10.13286/j.cnki.chinhosppharmacyj.2015.09.06.
许群, 眭建.免疫抑制小鼠肺白念珠菌感染模型的建立.中国老年学杂志, 2012, 32(10): 2111-2113. DOI: 10.3969/j.issn.1005-9202.2012.10.053.
XUQ, SUI J. Establishment of an immunosuppressive mouse model of Candida albicans infection. Chin J Gerontol, 2012, 32(10): 2111-2113.DOI: 10.3969/j.issn.1005-9202.2012.10.053.XUDOI: 10.16190/j.cnki.45-1211/r.2017.04.006.
ZHENG X J, LYU J Y, LI K, et al . Establishment and evaluation of rat model of cerebral infarction complicated with bacterial pneumonia. J Guangxi Med Univ, 2017, 34(4): 504-509. DOI: 10.16190/j.cnki.45-1211/r.2017.04.006.
贾晓峰, 郭新彪.改进型快速小鼠气管滴注法——透射灯下经口直视滴注法研究.环境与健康杂志, 2012, 29(3): 217-219.
JIA X F, GUO X B. Improved technique for rapid endotracheal instillation in mice: directviewing intubation through oropharynx under the trans-illumination. J Environment Health, 2012, 29(3): 217-219.
PEARSON J P, FELDMAN M, IGLEWSKI B H, et al . Pseudomonas aeruginosa cell-to-cell signaling is required for virulence in a model of acute pulmonary infection. Infect Immun, 2000, 68(7): 4331-4334.
EVANS T J. Small colony variants of Pseudomonas aeruginosa in chronic bacterial infection of the lung in cystic fibrosis. Future Microbiol, 2015, 10(2): 231-239. DOI: 10.2217/fmb.14.107.
FENG W, SUN F J, WANG Q, et al . Epidemiology and resistance characteristics of Pseudomonas aeruginosa isolates from the respiratory department of a hospital in China. J Glob Antimicrob Resist, 2017, 8: 142-147. DOI: 10.1016/j.jgar.2016.11.012.
李美凤, 封继宏, 魏葆琳.肺炎动物模型研究近况.辽宁中医杂志, 2014, 41(3): 601-604. DOI: 10.13192/j.issn.1000-1719.2014.03.091.
LIM F, FENG J H, WEI B L. Recent research on animal models of pneumonia. Liaoning J Traditional Chin Med, 2014, 41(3): 601-604. DOI: 10.13192/j.issn.1000-1719.2014.03.091.
GRIMWOOD K, KYD J M, OWEN S J, et al . Vaccination against respiratory Pseudomonas aeruginosa infection. Hum Vaccin Immunother, 2014, 11(1): 14-20. DOI: 10.4161/hv.34296.
佟若菲, 陈朝晖.铜绿假单胞菌感染的抗菌药物治疗.天津药学, 2012, 24(1): 70-73. DOI: 10.3969/j.issn.1006-5687.2012.01.029.
TONGR F, CHEN Z H. Antibacterial therapy for Pseudomonas aeruginosa infection. Tianjin Pharm, 2012, 24(1): 70-73. DOI: 10.3969/j.issn.1006-5687.2012.01.029.
宋秀杰, 彭娅, 王睿.肺炎链球菌肺炎动物模型研究进展.军医进修学院学报, 2009, 30(2): 234-235.
SONG X J, PENG Y, WANG R. Progress in research of animal streptococcus pneumonia model. Acad J PLA Postgrad Med School, 2009, 30(2): 234-235.
BERGERON Y, DESLAURIERS A M, OUELLET N, et al . Influence of cefodizime on pulmonary inflammatory response to heat-killed Klebsiella pneumoniae in mice.Antimicrob Agents Chemother, 1999, 43(9): 2291-2294.
张怡敏, 周雪宁, 张宏方, 等.小鼠鲍曼不动杆菌肺感染模型的制备.细胞与分子免疫学杂志, 2017, 33(10): 1392-1397.
ZHANG Y M, ZHOU X N, ZHANG H F, et al . Establishment of Acinetobacter baumannii-induced pneumonia model in mice. Chin J Cell Mol Immunol, 2017, 33(10): 1392-1397.
SCHMIEDL A, KERBER-MOMOT T, MUNDER A, et al . Bacterial distribution in lung parenchyma early after pulmonary infection with Pseudomonas aeruginosa. Cell Tissue Res, 2010, 342(1): 67-73. DOI: 10.1007/s00441-010-1036-y.
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