非小细胞肺癌患者外周血中lncRNA NEAT1的表达及诊断意义

郭晓平; 向颖; 袁帅; 吴龙; 邬娜; 李成英; 许斌; 余祖滨; 白莉; 杨敬源; 李亚斐

doi:10.16016/j.1000-5404.201811052

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非小细胞肺癌患者外周血中lncRNA NEAT1的表达及诊断意义

临床医学 | 更新时间：2026-01-09

- 非小细胞肺癌患者外周血中lncRNA NEAT1的表达及诊断意义
- Expression and diagnostic significance of lncRNA NEAT1 in peripheral blood of patients with non-small cell lung cancer
- 第三军医大学学报 2019年41卷第5期页码：448-453
- 作者机构：
  
  1.550000 贵阳，贵州医科大学公共卫生学院流行病学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队流行病学教研室
  3.400037 重庆，陆军军医大学(第三军医大学)第二附属医院：胸外科
  4.400037 重庆，陆军军医大学(第三军医大学)第二附属医院：全军呼吸内科研究所，全军呼吸病研究重点实验室
- 作者简介：
  
  [杨敬源，E-mail:yang8880@sina.comYANG Jingyuan, E-mail:yang8880@sina.com
  [李亚斐，E-mail:liyafei2008@hotmail.comLI Yafei, E-mail:liyafei2008@hotmail.com
- 基金信息：
  
  国家自然科学基金面上项目(81871896);国家自然科学基金面上项目(81672316);国家自然科学基金面上项目(81472190);国家自然科学基金(81602933)
- DOI：10.16016/j.1000-5404.201811052
  中图分类号： R342.2;R730.43;R734.2
- 收稿：2018-11-08，
  
  修回：2018-12-10，
  
  纸质出版：2019-03-15
- 稿件说明：
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郭晓平, 向颖, 袁帅, 等. 非小细胞肺癌患者外周血中lncRNA NEAT1的表达及诊断意义[J]. 第三军医大学学报, 2019,41(5):448-453.

Xiaoping GUO, Ying XIANG, Shuai YUAN, et al. Expression and diagnostic significance of lncRNA NEAT1 in peripheral blood of patients with non-small cell lung cancer[J]. Journal of Third Military Medical University, 2019, 41(5): 448-453.
郭晓平, 向颖, 袁帅, 等. 非小细胞肺癌患者外周血中lncRNA NEAT1的表达及诊断意义[J]. 第三军医大学学报, 2019,41(5):448-453. DOI： 10.16016/j.1000-5404.201811052.

Xiaoping GUO, Ying XIANG, Shuai YUAN, et al. Expression and diagnostic significance of lncRNA NEAT1 in peripheral blood of patients with non-small cell lung cancer[J]. Journal of Third Military Medical University, 2019, 41(5): 448-453. DOI： 10.16016/j.1000-5404.201811052.

摘要

目的

探究肺癌患者血浆中lncRNA NEAT1的表达水平以及对非小细胞肺癌的诊断价值。

方法

采用qRT-PCR技术检测258例非小细胞肺癌患者、115例肺部良性病变患者及70例健康对照外周血血浆样本中NEAT1的表达水平，比较其在血浆中的差异表达；分析NEAT1表达水平与肺癌患者临床特征的相关性；绘制ROC曲线分析血浆NEAT1水平对非小细胞肺癌的诊断价值。

结果

非小细胞肺癌患者血浆NEAT1水平较对照样本高表达(

0.01)；血浆NEAT1水平与非小细胞肺癌患者年龄、肿瘤大小相关，在

60岁组及肿瘤大小

3 cm组高表达，差异具有统计学意义(

0.05)；ROC曲线结果显示血浆NEAT1水平对区分非小细胞肺癌、早期非小细胞肺癌(Ⅰ期+Ⅱ期)、肿瘤大小≤3 cm的非小细胞肺癌与对照均有诊断价值，ROC曲线下面积分别为0.72、0.73、0.67。血浆NEAT1水平对于区分肿瘤大小≤3 cm的非小细胞肺癌与对照具有较高的灵敏度(93%)和阴性预测值(94%)。

结论

NEAT1在非小细胞肺癌血浆中高表达，NEAT1具有作为非小细胞肺癌诊断标志物的潜能及肺癌早期辅助诊断价值。

Abstract

Objective

To investigate the expression level and diagnostic value of lncRNA NEAT1 in the plasma of patients with non-small cell lung cancer (NSCLC).

Methods

qRT-PCR was used to detect the expression level of NEAT1 in plasma samples of 258 patients with NSCLC

115 patients with benign lung diseases and 70 healthy controls

and then the differential expression levels of NEAT1 were compared in the 3 groups. The correlation between the expression level of NEAT1 in plasma and the clinical characteristics of patients with NSCLC was analyzed. Receiver operating characteristic (ROC) curve analysis was used to analyze the diagnostic value of the NEAT1 level in plasma for NSCLC.

Results

In the NSCLC patients

the plasma level of NEAT1 was significantly higher than th

at in the healthy controls (

0.01). The expression level of NEAT1 in the plasma was correlated with the age and tumor size of NSCLC patients

and was highly expressed in the

60 year-old group and the group whith tumor size

3 cm

with statistically significant differences (

0.05). ROC curve results showed that plasma NEAT1 level had value for diagnosis of NSCLC

early stage NSCLC (stageⅠ+stageⅡ) or patients whose tumor size was equal to or less than 3 cm

with the areas under the curve of 0.72

0.73 and 0.67

respectively. The plasma level of NEAT1 had a higher sensitivity (93%) as well as a higher negative predictive value (94%) to distinguish NSCLC with the tumor size ≤3 cm.

Conclusion

NEAT1 is highly expressed in the plasma of NSCLC patients. NEAT1 could be used as a potential diagnostic marker for NSCLC

and shows early diagnostic value for lung cancer.

关键词

Keywords

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