mir-151a-5p对肺癌A549细胞生物学功能和化疗敏感性的影响

隆姝孜; 周寒静; 刘静姝; 刘洪良; 万林; 周丽; 张涛

doi:10.16016/j.1000-5404.201811041

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mir-151a-5p对肺癌A549细胞生物学功能和化疗敏感性的影响

基础医学 | 更新时间：2026-03-02

- mir-151a-5p对肺癌A549细胞生物学功能和化疗敏感性的影响
- Down-regulation of microRNA-151a-5p inhibits proliferation, migration and invasion and enhances chemosensitivity of lung cancer A549 cells
- 第三军医大学学报 2019年41卷第8期页码：770-777
- 作者机构：
  
  1..400016 重庆，重庆医科大学附属第一医院：肿瘤科Department of Oncology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China
  2..400016 重庆，重庆医科大学附属第一医院：胃肠外科Department of Gastrointestinal Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China
- 作者简介：
- 基金信息：
  
  重庆市卫生计生委医学科研计划项目(2017MSXM005)
- DOI：10.16016/j.1000-5404.201811041
  中图分类号： R394.3;R730.23;R734.2
- 收稿：2018-11-07，
  
  纸质出版：2019-04-30
- 稿件说明：
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隆姝孜, 周寒静, 刘静姝, 等. mir-151a-5p对肺癌A549细胞生物学功能和化疗敏感性的影响[J]. 第三军医大学学报, 2019,41(8):770-777.

Shuzi LONG, Hanjing ZHOU, Jingshu LIU, et al. Down-regulation of microRNA-151a-5p inhibits proliferation, migration and invasion and enhances chemosensitivity of lung cancer A549 cells[J]. Journal of Third Military Medical University, 2019, 41(8): 770-777.
隆姝孜, 周寒静, 刘静姝, 等. mir-151a-5p对肺癌A549细胞生物学功能和化疗敏感性的影响[J]. 第三军医大学学报, 2019,41(8):770-777. DOI： 10.16016/j.1000-5404.201811041.

Shuzi LONG, Hanjing ZHOU, Jingshu LIU, et al. Down-regulation of microRNA-151a-5p inhibits proliferation, migration and invasion and enhances chemosensitivity of lung cancer A549 cells[J]. Journal of Third Military Medical University, 2019, 41(8): 770-777. DOI： 10.16016/j.1000-5404.201811041.

摘要

目的

通过下调mir-151a-5p在肺癌A549细胞中的表达，研究其对细胞增殖、迁移、侵袭能力和化疗敏感性的影响。

方法

应用RT-qPCR检测mir-151a-5P在肺腺癌组织和肺腺癌细胞株以及转染inhibitor后的表达情况。通过CCK-8、Transwell、克隆形成实验分别检测转染inhibitor后A549细胞的增殖、迁移、侵袭能力以及克隆形成能力的变化。Western blot检测转染后EMT相关蛋白的表达。CCK-8、流式细胞术检测转染后A549细胞对顺铂的敏感性变化。

结果

mir-151a-5p在人肺腺癌组织中的表达较正常肺组织明显升高(

0.01)，且在4种肺癌细胞株(H1975、H1299、H2228、A549)中，A549细胞中mir-151a-5p表达量较高(

0.01)。成功构建了mir-151a-5p-inhibitor转染的A549细胞(

0.01)。mir-151a-5p inhibitor转染组较NC对照组细胞的增殖能力、迁移侵袭能力以及克隆形成能力都明显降低(

0.05)。Western blot检测结果显示，与NC对照组比较，mir-151a-5p inhibitor转染组E-cadherin表达明显升高，而N-cadherin、Vimentin、Notch1表达明显下降(

0.01)。CCK-8结果显示mir-151a-5p inhibitor转染组IC

显著低于NC对照组(

0.01)。流式细胞术检测结果显示，加入顺铂后mir-151a-5p inhibitor转染组凋亡率显著高于NC对照组(

0.01)。

结论

下调mir-151a-5p能显著抑制A549细胞的增殖、迁移及侵袭能力，并能够增加对顺铂的化疗敏感性。

Abstract

Objective

To investigate the effects of mir-151a-5p down-regulation on the proliferation

migration

invasion and chemosensitivity of A549 cells.

Methods

RT-qPCR was used to detect the expression of mir-151a-5P in 16 surgical specimens of lung adenocarcinoma and in different lung adenocarcinoma cell lines (H1975

H1299

H2228

A549 and HBE). CCK-8 assay

Transwell test and clonogenic assay were used to assess the changes in the proliferation

migration

invasion and clonogenic ability of A549 cells after transfection with a mir-151a-5p inhibitor. Western blotting was performed to detect the expression of epithelial-mesenchymal transition (EMT)-related proteins in the transfected cells; CCK-8 assay and flow cytometry were used to evaluate the changes in cisplatin sensitivity of A549 cells after the transfection.

Results

The expression of mir-151a-5p was significantly increased in human lung adenocarcinoma tissues as compared with normal lung tissues (

0.01). Among the 4 lung adenocarcinoma cell lines

A549 cells expressed the highest levels of mir-151a-5p (

0.01). Transfection with the mir-151a-5p inhibitor

compared with the negative control

significantly attenuated the proliferation

migration

invasion and clonogenic ability of A549 cells (

0.05). Western blotting revealed a significant enhancement of E-cadherin expression and obviously lowered expressions of N-candherin

vimentin and Notch1 in A549 cells transfected with the mir-151a-5p inhibitor (

0.01). CCK-8 assay showed that the IC

of cisplatin was significantly lowered in mir-151a-5p inhibitor-transfected cells as compared with the negative control group (

0.01); the transfection also resulted in a significantly increased apoptotic ra

te of the cells in response to cisplatin treatment (

0.01).

Conclusion

Down-regulation of mir-151a-5p expression can significantly inhibit the proliferation

migration and invasion of A549 cells and increase their chemosensitivity to cisplatin.

关键词

Keywords

references

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