姜黄素抑制TNF-α诱导的人脐带间充质干细胞凋亡

吴万江; 袁继超; 陈亚星; 倪飞; 陈欣; 王洁; 文泽贤; 张洪燕; 储卫华; 林江凯

doi:10.16016/j.1000-5404.201810068

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姜黄素抑制TNF-α诱导的人脐带间充质干细胞凋亡

基础医学 | 更新时间：2026-01-09

- 姜黄素抑制TNF-α诱导的人脐带间充质干细胞凋亡
- Curcumin inhibits tumor necrosis factor-α-induced apoptosis of human umbilical cord mesenchymal stem cells in vitro
- 第三军医大学学报 2019年41卷第4期页码：282-288
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)第一附属医院：神经外科，全军神经外科研究所，全军神经创伤防治重点实验室
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院：全军眼科中心
- 作者简介：
  
  [ 林江凯，E-mail: jklin@tmmu.edu.cn LIN Jiangkai, E-mail: jklin@tmmu.edu.cn
- 基金信息：
  
  国家自然科学基金面上项目(81571214);西南医院科技创新计划(SWH2016JSYB-03)
- DOI：10.16016/j.1000-5404.201810068
  中图分类号： R285.5;R329-3;R329.28
- 收稿：2018-10-12，
  
  修回：2018-11-12，
  
  纸质出版：2019-02-28
- 稿件说明：
移动端阅览
吴万江, 袁继超, 陈亚星, 等. 姜黄素抑制TNF-α诱导的人脐带间充质干细胞凋亡[J]. 第三军医大学学报, 2019,41(4):282-288.

Wanjiang WU, Jichao YUAN, Yaxing CHEN, et al. Curcumin inhibits tumor necrosis factor-α-induced apoptosis of human umbilical cord mesenchymal stem cells in vitro[J]. Journal of Third Military Medical University, 2019, 41(4): 282-288.
吴万江, 袁继超, 陈亚星, 等. 姜黄素抑制TNF-α诱导的人脐带间充质干细胞凋亡[J]. 第三军医大学学报, 2019,41(4):282-288. DOI： 10.16016/j.1000-5404.201810068.

Wanjiang WU, Jichao YUAN, Yaxing CHEN, et al. Curcumin inhibits tumor necrosis factor-α-induced apoptosis of human umbilical cord mesenchymal stem cells in vitro[J]. Journal of Third Military Medical University, 2019, 41(4): 282-288. DOI： 10.16016/j.1000-5404.201810068.

摘要

目的

探讨姜黄素(curcumin，cur)对TNF-α诱导人脐带间充质干细胞(human umbilical cord mesenchymal stem cells，hUC-MSC)凋亡的作用及机制。

方法

体外培养人脐带间充质干细胞，给予5 ng/mL TNF-α诱导细胞凋亡，给予不同浓度cur干预，乳酸脱氢酶(LDH)释放实验检测细胞凋亡情况；选取4 μmol/L作为干预剂量，采用流式细胞仪检测细胞凋亡情况，进一步验证cur对TNF-α诱导的hUC-MSC凋亡的作用。Western blot检测肿瘤坏死因子-α受体(tumor necrosis factor receptor，TNFR)下游凋亡相关的MAPK家族信号通路(ERK、JNK、p38)激活情况及Bcl-2、Bax的表达。

结果

LDH释放实验结果提示：当TNF-α浓度高于5 ng/mL时导致hUC-MSC凋亡。cur浓度在0.5~4 μmol/L时可抑制TNF-α诱导的hUC-MSC凋亡，且呈浓度依赖性。流式细胞仪检测结果也证实4 μmol/L cur能显著抑制TNF-α诱导的hUC-MSC凋亡(

0.05)。Western blot检测结果显示：cur可以激活ERK，促进Bcl-2的表达，使Bcl-2/Bax的表达升高(

0.05)，但不影响MAPK家族JNK、p38蛋白表达。

结论

姜黄素能抑制TNF-α诱导的hUC-MSC凋亡，其作用机制可能是通过激活ERK，促进Bcl-2表达而实现。

Abstract

Objective

To investigate the inhibitory effect of curcumin on tumor necrosis factor-α (TNF-α)-induced apoptosis of human umbilical cord mesenchymal stem cells (hUC-MSCs) and explore the underlying mechanism.

Methods

hUC-MSCs cultured

in vitro

were treated with 5 ng/mL TNF-α and different concentrations of curcumin. LDH release assay and flow cytometry were used to assess the inhibitory effect of curcumin against TNF-α-induced cell apoptosis. Western blotting was used to detect the expression of the apoptosis-related molecules in the downstream MAPK signaling pathway (ERK

JNK

and p38) of tumor necrosis factor receptor (TNFR) and the apoptosis-related proteins (Bcl-2 and Bax).

Results

LDH release assay showed that TNF-α at a concentration above 5 ng/mL induced obvious apoptosis in cultured hUC-MSCs. Curcumin at the concentrations between 0.5 and 4 μmol/L significantly inhibited TNF-α-induced hUC-MSC apoptosis in a concentration-dependent manner; Flow cytometry also confirmed that 4 μmol/L curcumin significantly inhibited TNF-α-induced apoptosis of hUC-MSCs (

0.05). The results of Western blotting showed that curcumin treatment caused ERK activation and enhanced the expression of Bcl-2 to increase the Bcl-2/Bax ratio (

0.05) without obviously affecting the expression of JNK and p38 proteins in the cells.

Conclusion

Curcumin can inhibit TNF-α-induced apoptosis of hUC-MSCs possibly by activating ERK signaling pathway and enhancing Bcl-2 expression.

关键词

Keywords

references

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