RNAi沉默S100A9对U87胶质瘤干细胞增殖和自我更新的影响

陈宸; 陈松; 霍钢

doi:10.16016/j.1000-5404.201810047

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RNAi沉默S100A9对U87胶质瘤干细胞增殖和自我更新的影响

基础医学 | 更新时间：2026-01-09

- RNAi沉默S100A9对U87胶质瘤干细胞增殖和自我更新的影响
- Effect of RNA interference-mediated S100A9 gene silencing on proliferation and self-renewal of U87 glioma stem cells in vitro
- 第三军医大学学报 2019年41卷第6期页码：575-580
- 作者机构：
  
  400016 重庆，重庆医科大学附属第一医院神经外科
- 作者简介：
  
  [ 霍钢，E-mail：xiaomin198171@tom.com HUO Gang, E-mail: xiaomin198171@tom.com
- 基金信息：
  
  重庆市基础科学与前沿技术研究专项(CSTC2016jcyjA0838)
- DOI：10.16016/j.1000-5404.201810047
  中图分类号： R394.3;R730.23;R730.264
- 收稿：2018-10-10，
  
  修回：2018-11-18，
  
  纸质出版：2019-03-30
- 稿件说明：
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陈宸, 陈松, 霍钢. RNAi沉默S100A9对U87胶质瘤干细胞增殖和自我更新的影响[J]. 第三军医大学学报, 2019,41(6):575-580.

Chen CHEN, Song CHEN, Gang HUO. Effect of RNA interference-mediated S100A9 gene silencing on proliferation and self-renewal of U87 glioma stem cells in vitro[J]. Journal of Third Military Medical University, 2019, 41(6): 575-580.
陈宸, 陈松, 霍钢. RNAi沉默S100A9对U87胶质瘤干细胞增殖和自我更新的影响[J]. 第三军医大学学报, 2019,41(6):575-580. DOI： 10.16016/j.1000-5404.201810047.

Chen CHEN, Song CHEN, Gang HUO. Effect of RNA interference-mediated S100A9 gene silencing on proliferation and self-renewal of U87 glioma stem cells in vitro[J]. Journal of Third Military Medical University, 2019, 41(6): 575-580. DOI： 10.16016/j.1000-5404.201810047.

摘要

目的

探讨沉默S100A9蛋白对人U87胶质瘤干细胞增殖和自我更新能力的影响和机制。

方法

采用shRNA-S100A9慢病毒感染人U87胶质瘤干细胞，RT-qPCR检测S100A9 mRNA表达，Western blot检测S100A9蛋白表达，MTT法检测细胞的增殖情况，干细胞球形成实验检测自我更新能力，RT-qPCR和Western blot检测β-catenin mRNA和蛋白的表达。

结果

慢病毒感染后的细胞组S100A9 mRNA和蛋白表达均受到了明显抑制(

0.05)，细胞增殖变慢(

0.05)，干细胞球形成能力受到明显抑制(

0.05)，β-catenin mRNA和蛋白的表达明显下调(

0.05)。

结论

抑制S100A9蛋白的表达能明显抑制U87胶质瘤干细胞增殖和自我更新能力，其机制可能与抑制Wnt/β-catenin信号转导通路有关。

Abstract

Objective

To investigate the effect of S100A9 gene silencing on proliferation and self-renewal ability of human U87 glioma stem cells.

Methods

Human U87 glioma stem cells were infected with shRNA-S100A9 lentivirus for S100A9 gene silencing

and the changes in the expression of S100A9 at mRNA and protein levels were analyzed using RT-qPCR and Western blotting

respectively. The changes in the proliferation of the cells were observed using MTT assay

and the self-renewal ability of the stem cells was assessed using stem cell sphere formation assay. The expression of β-catenin at the mRNA and protein levels was detected using RT-qPCR and Western blotting.

Results

Infection of U87 cells with shRNA-S100A9 lentivirus caused significant inhibition of the expression of S100A9 at both the mRNA and protein levels (

0.05) and obviously suppressed the cell proliferation (

0.05) and stem cell sphere formation (

0.05). S100A9 gene silencing also significantly down-regulated the mRNA and protein expression of β-catenin in U87 cel

ls (

0.05).

Conclusion

S100A9 gene silencing significantly inhibits the proliferation and self-renewal ability of U87 glioma stem cells

in vitro

关键词

Keywords

references

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