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GClnc1通过SIRT3/FOXO3/SOD2信号通路促进胃癌细胞SGC7901的增殖和迁移
GClnc1 promotes proliferation and migration of human gastric cancer cells
in vitro by activating SIRT3/FOXO3/SOD2 pathway- 第三军医大学学报 2019年41卷第7期 页码：673-677
- 作者机构：
1.510000 广州，广州市第十二人民医院消化内科
2.518000 广东 深圳，深圳市南山区人民医院肿瘤科
- 作者简介：
[ 宋慧东, E-mail：2354412112@qq.com SONG Huidong, E-mail:2354412112@qq.com
- 基金信息：深圳市南山区技术研发和创意设计项目分项资金教育(卫生)科技资助项目(南科研卫2018027号)
DOI：10.16016/j.1000-5404.201810033
中图分类号： R394.2;R730.23;R735.2收稿：2018-10-09，
修回：2018-12-10，
纸质出版：2019-04-15
- 稿件说明：
移动端阅览
卢丽琴, 付晓红, 宋慧东. GClnc1通过SIRT3/FOXO3/SOD2信号通路促进胃癌细胞SGC7901的增殖和迁移[J]. 第三军医大学学报, 2019,41(7):673-677.
Liqin LU, Xiaohong FU, Huidong SONG. GClnc1 promotes proliferation and migration of human gastric cancer cells
in vitro by activating SIRT3/FOXO3/SOD2 pathway[J]. Journal of Third Military Medical University, 2019, 41(7): 673-677.卢丽琴, 付晓红, 宋慧东. GClnc1通过SIRT3/FOXO3/SOD2信号通路促进胃癌细胞SGC7901的增殖和迁移[J]. 第三军医大学学报, 2019,41(7):673-677. DOI： 10.16016/j.1000-5404.201810033.
Liqin LU, Xiaohong FU, Huidong SONG. GClnc1 promotes proliferation and migration of human gastric cancer cells
in vitro by activating SIRT3/FOXO3/SOD2 pathway[J]. Journal of Third Military Medical University, 2019, 41(7): 673-677. DOI： 10.16016/j.1000-5404.201810033.
摘要
目的
2
探究lncRNA GClnc1是否通过SIRT3/FOXO3/SOD2调控胃癌细胞增殖和迁移。
方法
2
通过转染慢病毒的方法，在人胃癌细胞SGC7901中分别过表达和沉默GClnc1，采用Western blot和qRT-PCR检测SIRT3/FOXO3/SOD2 mRNA和蛋白水平的改变；在过表达GClnc1的同时分别沉默SIRT3/FOXO3/SOD2，采用CCK-8检测细胞增殖能力，划痕实验检测细胞迁移能力。
结果
2
过表达GClnc1可以上调SIRT3/FOXO3/SOD2的mRNA和蛋白水平(
P
<
0.05)，并增强SGC7901细胞的增殖[(1.00±0.14)
vs
(1.79±0.21)
P
<
0.05
]
和迁移能力(10.87±0.76
vs
16.53±2.25
P
<
0.05)，而沉默GClnc1则降低SIRT3/FOXO3/SOD2的mRNA和蛋白水平(
P
<
0.05)，并减少细胞增殖(1.00±0.14
vs
0.62±0.13
P
<
0.05)和迁移能力(10.87±0.76
vs
6.46±0.89
P
<
0.01)。沉默SIRT3/FOXO3/SOD2可以消除过表达GClnc1对细胞增殖和迁移能力的促进作用。
结论
2
GClnc1通过激活SIRT3/FOXO3/SOD2信号通路促进SGC7901细胞增殖和迁移。
Abstract
Objective
2
To investigate whether the long noncoding RNA (lncRNA) GClnc1 regulates the proliferation and migration of gastric cancer cells by modulating the expression of SIRT3/FOXO3/SOD2 pathway.
Methods
2
We transfected human gastric cancer SGC7901 cells with lentiviral vectors to induce overexpression or knockdown of GClnc1
and examined the changes in the expression of SIRT3/FOXO3/SOD2 at both mRNA and protein levels using qRT-PCR and Western blotting. We also observed the effects of SIRT3/FOXO3/SOD2 silencing on the proliferation and migration of GClnc1-overexpressing SGC7901 cells using CCK8 assay and scratch wound healing assay
respectively.
Results
2
Overexpression of GClnc1 significantly up-regulated the mRNA and protein levels of SIRT3/FOXO3/SOD2 (
P
<
0.05)
and obviously enhanced the proliferation and migration of SGC7901 cells (
P
<
0.05). GClnc1 knockdown significantly lowered the the mRNA and protein levels of SIRT3/FOXO3/SOD2 (
P
<
0.05) and inhibited the proliferation and migration of the cells (
P
<
0.05). Silencing SIRT3/FOXO3/SOD2 obviously eliminated the effect of GClnc1 overexpression on the cell proliferation and migration.
Conclusion
2
GClnc1 promotes proliferation and migration of SGC7901 cells by activating SIRT3/FOXO3/SOD2 pathway.
关键词
Keywords
references
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