浏览全部资源
扫码关注微信
200例CDHR3基因单核苷酸多态性与人鼻病毒所致婴幼儿下呼吸道感染的关系
Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases
- 第三军医大学学报 2019年41卷第4期 页码：371-375
- 作者机构：
1.400014 重庆，重庆医科大学附属儿童医院儿科研究所，儿童发育疾病研究教育部重点实验室，儿童发育重大疾病国家国际科技合作基地，儿童感染免疫重庆市重点实验室
2.401122 重庆，重庆医科大学附属儿童医院呼吸中心
- 作者简介：
[ 刘恩梅, E-mail：emliu186@126.com LIU Enmei, E-mail: emliu186@126.com
- 基金信息：
DOI：10.16016/j.1000-5404.201809195
中图分类号： R181.32;R394.1;R725.62收稿：2018-09-27，
修回：2018-11-14，
纸质出版：2019-02-28
- 稿件说明：
移动端阅览
李慧, 任洛, 张瑶, 等. 200例CDHR3基因单核苷酸多态性与人鼻病毒所致婴幼儿下呼吸道感染的关系[J]. 第三军医大学学报, 2019,41(4):371-375.
Hui LI, Luo REN, Yao ZHANG, et al. Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases[J]. Journal of Third Military Medical University, 2019, 41(4): 371-375.
李慧, 任洛, 张瑶, 等. 200例CDHR3基因单核苷酸多态性与人鼻病毒所致婴幼儿下呼吸道感染的关系[J]. 第三军医大学学报, 2019,41(4):371-375. DOI： 10.16016/j.1000-5404.201809195.
Hui LI, Luo REN, Yao ZHANG, et al. Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases[J]. Journal of Third Military Medical University, 2019, 41(4): 371-375. DOI： 10.16016/j.1000-5404.201809195.
摘要
目的
2
探讨CDHR3基因单核苷酸多态性与人鼻病毒(human rhinoviruses
HRV)所致儿童急性下呼吸道感染的关系。
方法
2
收集2012年1月至2014年11月因HRV所致急性下呼吸道感染的患儿鼻咽抽吸物，提取RNA逆转录后，采用PCR方法扩增CDHR3基因片段，测序后进行rs6967330位点基因分型。利用PCR的方法扩增HRV的VP4基因进行测序分型，实时荧光定量PCR方法进行HRV载量测定，并回顾性分析rs6967330位点与患儿临床特征的相关性。
结果
2
共纳入200例患儿，其中AA/AG基因型30例(15.0%)，GG基因型170例(85.0%)。HRV所致急性下呼吸道感染患儿中，AA/AG基因型与GG基因型相比更容易出现喘息症状(86.7%
vs
57.1%，
P
=0.002)，而且更易致重症肺炎(30.0%
vs
11.2%，
P
=0.018)，一级亲属也更多具有特应性疾病病史(16.7%
vs
5.88%，
P
=0.039)。Logistic回归分析发现rs6967330 A是HRV感染患儿喘息(
OR
: 4.585，95%
CI
: 1.446 ~ 14.541，
P
=0.010)及发展为重症病例(
OR
: 3.379，95%
CI
: 1.009 ~ 11.315，
P
=0.048)的危险因素。而HRV病毒亚型及载量在不同基因型间差异没有统计学意义。
结论
2
CDHR3基因单核苷酸多态性位点rs6967330可能与HRV感染后的重症及喘息相关。
Abstract
Objective
2
To explore the association of single nucleotide polymorphism (SNP) at rs6967330 of cadherin-associated family member 3 (CDHR3) gene with acute respiratory tract infection caused by human rhinoviruses (HRVs) in children.
Methods
2
From January
2012 to November
2014
we collected specimens of nasopharyngeal aspirates from 200 children with acute lower respiratory tract infection caused by HRVs. CDHR3 gene fragment was amplified by PCR from the samples and sequenced for rs6967330 genotyping. The VP4 gene of HRV was amplified with PCR for subsequent sequencing
and real-time fluorescent quantitative PCR was used to estimate the virus load. The correlation between rs6967330 genotypes and the clinical features of the children was analyzed.
Results
2
Of the 200 children with acute lower respiratory tract infection caused by HRVs
30 (15.0%) had AA/AG genotype and 170 (85.0%) had GG genotype of rs6967330. The children with AA/AG genotype were more likely to have wheezing symptoms than those with GG genotype (86.7%
vs
57.1%
P
=0.002)
and were also more susceptible to severe pneumonia (30.0%
vs
11.2%
P
=0.018); the first-degree relatives of the children with AA/AG genotype were also more likely to have a history of atopic diseases(16.7%
vs
5.88%
P
=0.039). Logistic regression analysis revealed that rs6967330 A was a risk factor for wheezing in children with HRV infection (
OR
: 4.585
95%
CI
: 1.446~14.541
P
=0.010) and also for progression into severe cases (
OR
: 3.379
95%
CI
: 1.009~11.315
P
=0.048). No significant differences in HRV subtypes or viral loads were found between the children with different genotypes of rs6967330.
Conclusion
2
The SNP of CDHR3 gene at rs6967330 may be associated with severe cases and wheezing after lower respiratory tract infection with HRV in children.
关键词
Keywords
references
PALMENBERG A C.Rhinovirus C, asthma, and cell surface expression of virus receptor CDHR3[J]. J Virol, 2017, 91(7): e00072-C00017. DOI: 10.1128/JVI.00072-17.
JACOBS S E, LAMSON D M, ST GEORGE K, et al . Human rhinoviruses[J ] . Clin Microbiol Rev, 2013, 26(1): 135-162. DOI: 10.1128/CMR.00077-12.
郑首燕.支气管哮喘急性发作患儿鼻病毒检出率及其分子流行病学研究[D].重庆: 重庆医科大学, 2016. [http://cdmd.cnki.com.cn/Article/CDMD-10631-1016783224.htm .
ZHENG S Y. Detection rate of rhinovirus in children with acute asthma attack and its molecular epidemiology[D]. Chongqing: Chongqing Medical University, 2016.
KANAZAW A J, MASUKO H, YATAGAI Y, et al . Genetic association of the functional CDHR3 genotype with early-onset adult asthma in Japanese populations[J ] . Allergol Int, 2017, 66(4): 563-567. DOI: 10.1016/j.alit.2017.02.012.
BOCHKOV Y A, WATTERS K, ASHRAF S, et al . Cadherin-related family member 3, a childhood asthma susceptibility gene product, mediates rhinovirus C binding and replication[J ] . Proc Natl Acad Sci USA, 2015, 112(17): 5485-5490. DOI: 10.1073/pnas.1421178112.
HEIM A, EBNET C, HARSTE G, et al . Rapid and quantitative detection of human adenovirus DNA by real-time PCR[J ] . J Med Virol, 2003, 70(2): 228-239. DOI:10.1002/jmv.10382.
ALLANDER T, JARTTI T, GUPTA S, et al . Human bocavirus and acute wheezing in children[J ] . Clin Infect Dis, 2007, 44(7): 904-910. DOI: 10.1086/512196.
COIRAS M T, AGUILAR J C, GARC A M L, et al . Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays[J ] . J Med Virol, 2004, 72(3): 484-495. DOI: 10.1002/jmv.20008.
COIRAS M T, P REZ-BRE A P, GARC A M L, et al . Simultaneous detection of influenza A, B, and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested-PCRassay[J ] . J Med Virol, 2003, 69(1): 132-144. DOI: 10.1002/jmv.10255.
肖秋艳.重庆地区人鼻病毒和肠道病毒D68在呼吸道感染住院患儿中的分子流行病学研究[D].重庆: 重庆医科大学, 2015. [http://cdmd.cnki.com.cn/Article/CDMD-10631-1016007274.htm .
XIAO Q Y. Epidemiological analysis of human rhinovirus and enterovirus in hospitalized children with respiratory infections in Chongqing[D]. Chongqing: Chongqing Medical University, 2015.
CHANG E H, WILLIS A L, MCCRARY H C, et al . Association between the CDHR3 rs6967330 risk allele and chronic rhinosinusitis[J ] . J Allergy Clin Immunol, 2017, 139(6): 1990-1992.e2. DOI: 10.1016/j.jaci.2016.10.027.
ZHAO B, YU X, WANG C, et al . High human bocavirus viral load is associated with disease severity in children under five years of age[J ] . PLoS ONE, 2013, 8(4): e62318. DOI: 10.1371/journal.pone.0062318.
B NNELYKKE K, SLEIMAN P, NIELSEN K, et al . A genome-wide association study identifies CDHR3 as a susceptibility locus for early childhood asthma with severe exacerbations[J ] . Nat Genet, 2014, 46(1): 51-55. DOI: 10.1038/ng.2830.
BAI J, SMOCK S L, JACKSON G R Jr, et al . Phenotypic responses of differentiated asthmatic human airway epithelial cultures to rhinovirus[J ] . PLoS ONE, 2015, 10(2): e0118286. DOI: 10.1371/journal.pone.0118286.
STENBERG HAMMAR K, NIESPODZIANA K, VAN HAGE M, et al . Reduced CDHR3 expression in children wheezing with rhinovirus[J ] . Pediatr Allergy Immunol, 2018, 29(2): 200-206. DOI: 10.1111/pai.12858.
HUSBY A, PASANEN A, WAAGE J, et al . CDHR3 gene variation and childhood bronchiolitis[J ] . J Allergy Clin Immunol, 2017, 140(5): 1469-1471.e7. DOI: 10.1016/j.jaci.2017.06.044.
LAUINGER I L, BIBLE J M, HALLIGAN E P, et al . Patient characteristics and severity of human rhinovirus infections in children[J ] . J Clin Virol, 2013, 58(1): 216-220. DOI: 10.1016/j.jcv.2013.06.042
LINDER J E, KRAFT D C, MOHAMED Y, et al . Human rhinovirus C: age, season, and lower respiratory illness over the past 3 decades[J ] . J Allergy Clin Immunol, 2013, 131(1): 69-77.e1-6. DOI: 10.1016/j.jaci.2012.09.033.
ESPOSITO S, DALENO C, SCALA A, et al . Impact of rhinovirus nasopharyngeal viral load and viremia on severity of respiratory infections in children[J ] . Eur J Clin Microbiol Infect Dis, 2014, 33(1): 41-48. DOI: 10.1007/s10096-013-1926-5.
0
浏览量
0
下载量
0
CSCD
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
